Q07444 (NKG2E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NKG2-E type II integral membrane protein

Alternative name(s):
NK cell receptor E
NKG2-E-activating NK receptor

Gene names

Name:	KLRC3
Synonyms:	NKG2E

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Subunit structure	Can form disulfide-bonded heterodimer with CD94.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Natural killer cells.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular defense response Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sugar binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane signaling receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform NKG2-E (identifier: Q07444-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform NKG2-H (identifier: Q07444-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-240: RRGFIMLTRLVLNS → VSISFRIKALELAVHQIKFYICSNRNDIMIA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	NKG2-E type II integral membrane protein		PRO_0000046671
Regions
	Topological domain	1 – 70	70	Cytoplasmic Potential
	Transmembrane	71 – 93	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	94 – 240	147	Extracellular Potential
	Domain	116 – 230	115	C-type lectin
Amino acid modifications
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 128		By similarity
	Disulfide bond	207 ↔ 220		By similarity
Natural variations
	Alternative sequence	227 – 240	14	RRGFI…LVLNS → VSISFRIKALELAVHQIKFY ICSNRNDIMIA in isoform NKG2-H.		VSP_003067
	Natural variant	2	1	S → N. Ref.1 Ref.2 Ref.3 Ref.4 Corresponds to variant rs2682489 [ dbSNP | Ensembl ].		VAR_062954
	Natural variant	19	1	W → P in allele NKG2-E*01 and allele NKG2-E*03; requires 2 nucleotide substitutions. Ref.1 Ref.2 Ref.3		VAR_013296
	Natural variant	19	1	W → R in allele NKG2-E*02. Ref.4 Ref.5		VAR_063132
	Natural variant	106	1	A → T. Ref.1 Ref.3 Ref.4 Ref.5 Corresponds to variant rs28626640 [ dbSNP | Ensembl ].		VAR_063133
	Natural variant	113	1	H → P. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Corresponds to variant rs2682494 [ dbSNP | Ensembl ].		VAR_062955
	Natural variant	135	1	R → S. Corresponds to variant rs1138437 [ dbSNP | Ensembl ].		VAR_014660
	Natural variant	155	1	C → S. Ref.1 Ref.2 Ref.4 Ref.5 Corresponds to variant rs2682495 [ dbSNP | Ensembl ].		VAR_062956
Experimental info
	Sequence conflict	48	1	A → P in AAL65232. Ref.5
	Sequence conflict	134	1	E → G in AAL65232. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform NKG2-E [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 9F7056DD558A40C3	FASTA	240	27,100
	10 20 30 40 50 60 MSKQRGTFSE VSLAQDPKWQ QRKPKGNKSS ISGTEQEIFQ VELNLQNASL NHQGIDKIYD 70 80 90 100 110 120 CQGLLPPPEK LTAEVLGIIC IVLMATVLKT IVLIPFLEQN NSSPNARTQK ARHCGHCPEE 130 140 150 160 170 180 WITYSNSCYY IGKERRTWEE SLQACASKNS SSLLCIDNEE EMKFLASILP SSWIGVFRNS 190 200 210 220 230 240 SHHPWVTING LAFKHEIKDS DHAERNCAML HVRGLISDQC GSSRIIRRGF IMLTRLVLNS
	Isoform NKG2-H [UniParc]. Checksum: 1DFECB6179F43783	FASTA	257	29,018
	10 20 30 40 50 60 MSKQRGTFSE VSLAQDPKWQ QRKPKGNKSS ISGTEQEIFQ VELNLQNASL NHQGIDKIYD 70 80 90 100 110 120 CQGLLPPPEK LTAEVLGIIC IVLMATVLKT IVLIPFLEQN NSSPNARTQK ARHCGHCPEE 130 140 150 160 170 180 WITYSNSCYY IGKERRTWEE SLQACASKNS SSLLCIDNEE EMKFLASILP SSWIGVFRNS 190 200 210 220 230 240 SHHPWVTING LAFKHEIKDS DHAERNCAML HVRGLISDQC GSSRIIVSIS FRIKALELAV 250 HQIKFYICSN RNDIMIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Natural killer lectin-like receptors have divergent carboxy-termini, distinct from C-type lectins." Adamkiewicz T.V., McSherry C., Bach F.H., Houchins J.P. Immunogenetics 39:218-218(1994) [PubMed: 8276468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NKG2-E), VARIANTS ASN-2; PRO-19; THR-106; PRO-113 AND SER-155.
[2]	"The genomic organization of NKG2C, E, F, and D receptor genes in the human natural killer gene complex." Glienke J., Sobanov Y., Brostjan C., Steffens C., Nguyen C., Lehrach H., Hofer E., Francis F. Immunogenetics 48:163-173(1998) [PubMed: 9683661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM NKG2-E), VARIANTS ASN-2; PRO-19; PRO-113 AND SER-155.
[3]	"Triggering of effector functions on a CD8+ T cell clone upon the aggregation of an activatory CD94/kp39 heterodimer." Bellon T., Heredia A.B., Llano M., Minguela A., Rodriguez A., Lopez-Botet M., Aparicio P. J. Immunol. 162:3996-4002(1999) [PubMed: 10201920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NKG2-H), VARIANTS ASN-2; PRO-19; THR-106 AND PRO-113.
[4]	"Conservation and variation in human and common chimpanzee CD94 and NKG2 genes." Shum B.P., Flodin L.R., Muir D.G., Rajalingam R., Khakoo S.I., Cleland S., Guethlein L.A., Uhrberg M., Parham P. J. Immunol. 168:240-252(2002) [PubMed: 11751968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NKG2-E), VARIANTS ASN-2; ARG-19; THR-106; PRO-113 AND SER-155.
[5]	"Identification of the NKG2E gene in large granular lymphocytic leukemia (LGL)." Kothapalli R., Kusmartseva I., Loughran T.P. Jr. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ARG-19; THR-106; PRO-113 AND SER-155.
[6]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

NKG-2E

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14542 mRNA. Translation: AAA16833.1. AJ001685 Genomic DNA. Translation: CAA04923.1. AF078550 mRNA. Translation: AAD46108.1. AF350016 mRNA. Translation: AAK83803.1. AF350017 mRNA. Translation: AAK83804.1. AF461157 mRNA. Translation: AAL65232.1. AC022075 Genomic DNA. No translation available. AC068775 Genomic DNA. No translation available.
IPI	IPI00017478. IPI00219135.
PIR	I54524.
RefSeq	NP_002252.2. NM_002261.2. NP_031359.2. NM_007333.2. XP_003403690.1. XM_003403642.1.
UniGene	Hs.654362.
3D structure databases
ProteinModelPortal	Q07444.
SMR	Q07444. Positions 72-99, 111-225.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07444. 1 interaction.
STRING	Q07444.
Polymorphism databases
DMDM	296439288.
Proteomic databases
PRIDE	Q07444.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381904; ENSP00000371329; ENSG00000205810.
GeneID	3822. 3823.
KEGG	hsa:3822. hsa:3823.
UCSC	uc001qyf.1. human. uc001qyi.1. human.
Organism-specific databases
CTD	3822. 3823.
GeneCards	GC12M010564.
H-InvDB	HIX0201919.
HGNC	HGNC:6376. KLRC3.
MIM	602892. gene.
neXtProt	NX_Q07444.
PharmGKB	PA30165.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04679.
HOVERGEN	HBG077044.
Gene expression databases
ArrayExpress	Q07444.
Bgee	Q07444.
CleanEx	HS_KLRC3.
Genevestigator	Q07444.
GermOnline	ENSG00000205810. Homo sapiens.
Family and domain databases
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
KO	K06541.
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	15033.
SOURCE	Search...

Entry information

Entry name

NKG2E_HUMAN

Accession

Primary (citable) accession number: Q07444
Secondary accession number(s): Q8WXA4, Q96RL0, Q9UP04

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 104 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.