Reviewed, UniProtKB/Swiss-Prot Q07444 (NKG2E_HUMAN)

Names and origin

Protein names

Recommended name:
    NKG2-E type II integral membrane protein
Alternative name(s):
    NKG2-E-activating NK receptor
    NK cell receptor E

Gene names

Name:	KLRC3
Synonyms:	NKG2E

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Subunit structure	Can form disulfide-bonded heterodimer with CD94.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Natural killer cells.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cellular defense response Ref.2 Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sugar binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform NKG2-E (identifier: Q07444-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform NKG2-H (identifier: Q07444-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-240: RRGFIMLTRLVLNS → VSISFRIKALELAVHQIKFYICSNRNDIMIA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	NKG2-E type II integral membrane protein		PRO_0000046671
Regions
	Topological domain	1 – 70	70	Cytoplasmic Potential
	Transmembrane	71 – 93	23	Signal-anchor for type II membrane protein Potential
	Topological domain	94 – 240	147	Extracellular Potential
	Domain	116 – 230	115	C-type lectin
Amino acid modifications
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 128		By similarity
	Disulfide bond	207 ↔ 220		By similarity
Natural variations
	Alternative sequence	227 – 240	14	RRGFI…LVLNS → VSISFRIKALELAVHQIKFY ICSNRNDIMIA in isoform NKG2-H.		VSP_003067
	Natural variant	19	1	P → R in allele NKG2-E*02. Ref.4		VAR_013296
	Natural variant	135	1	R → S: dbSNP rs1138437.		VAR_014660

Sequences

	Sequence		Length	Mass (Da)
	Isoform NKG2-E [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 20691FB21274D8A6	FASTA	240	27,012
	10 20 30 40 50 60 MNKQRGTFSE VSLAQDPKPQ QRKPKGNKSS ISGTEQEIFQ VELNLQNASL NHQGIDKIYD 70 80 90 100 110 120 CQGLLPPPEK LTAEVLGIIC IVLMATVLKT IVLIPFLEQN NSSPNTRTQK ARPCGHCPEE 130 140 150 160 170 180 WITYSNSCYY IGKERRTWEE SLQACASKNS SSLLSIDNEE EMKFLASILP SSWIGVFRNS 190 200 210 220 230 240 SHHPWVTING LAFKHEIKDS DHAERNCAML HVRGLISDQC GSSRIIRRGF IMLTRLVLNS
	Isoform NKG2-H. Checksum: C2E8E70621B06EB9	FASTA	257	28,930
	10 20 30 40 50 60 MNKQRGTFSE VSLAQDPKPQ QRKPKGNKSS ISGTEQEIFQ VELNLQNASL NHQGIDKIYD 70 80 90 100 110 120 CQGLLPPPEK LTAEVLGIIC IVLMATVLKT IVLIPFLEQN NSSPNTRTQK ARPCGHCPEE 130 140 150 160 170 180 WITYSNSCYY IGKERRTWEE SLQACASKNS SSLLSIDNEE EMKFLASILP SSWIGVFRNS 190 200 210 220 230 240 SHHPWVTING LAFKHEIKDS DHAERNCAML HVRGLISDQC GSSRIIVSIS FRIKALELAV 250 HQIKFYICSN RNDIMIA

References

[1]	"Natural killer lectin-like receptors have divergent carboxy-termini, distinct from C-type lectins." Adamkiewicz T.V., McSherry C., Bach F.H., Houchins J.P. Immunogenetics 39:218-218(1994) [PubMed: 8276468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NKG2-E).
[2]	"The genomic organization of NKG2C, E, F, and D receptor genes in the human natural killer gene complex." Glienke J., Sobanov Y., Brostjan C., Steffens C., Nguyen C., Lehrach H., Hofer E., Francis F. Immunogenetics 48:163-173(1998) [PubMed: 9683661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM NKG2-E).
[3]	"Triggering of effector functions on a CD8+ T cell clone upon the aggregation of an activatory CD94/kp39 heterodimer." Bellon T., Heredia A.B., Llano M., Minguela A., Rodriguez A., Lopez-Botet M., Aparicio P. J. Immunol. 162:3996-4002(1999) [PubMed: 10201920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NKG2-H).
[4]	"Conservation and variation in human and common chimpanzee CD94 and NKG2 genes." Shum B.P., Flodin L.R., Muir D.G., Rajalingam R., Khakoo S.I., Cleland S., Guethlein L.A., Uhrberg M., Parham P. J. Immunol. 168:240-252(2002) [PubMed: 11751968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM NKG2-E), VARIANT ARG-19.

Web resources

Functional Glycomics Gateway - Glycan Binding

NKG-2E

Cross-references

Sequence databases
EMBLGenBankDDBJ	L14542 mRNA. Translation: AAA16833.1. AJ001685 Genomic DNA. Translation: CAA04923.1. AF078550 mRNA. Translation: AAD46108.1. AF350016 mRNA. Translation: AAK83803.1. AF350017 mRNA. Translation: AAK83804.1.
IPI	IPI00017478. IPI00219135.
PIR	I54524.
RefSeq	NP_002252.2. NP_031359.2.
UniGene	Hs.654362
3D structure databases
HSSP	HSSP built from PDB template 1B6E based on UniProtKB Q13241.
ModBase	Search...
Proteomic databases
PRIDE	Q07444.
Genome annotation databases
Ensembl	ENSG00000205810. Homo sapiens. [Contig view]
GeneID	3823.
KEGG	hsa:3823.
Organism-specific databases
GeneCards	GC12M010456.
H-InvDB	HIX0036694.
HGNC	HGNC:6376. KLRC3.
MIM	602892. gene.
PharmGKB	PA30165.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q07444.
Gene expression databases
ArrayExpress	Q07444.
Bgee	Q07444.
CleanEx	HS_KLRC3.
GermOnline	ENSG00000205810. Homo sapiens.
Family and domain databases
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	15033.
SOURCE	Search...

Entry information

Entry name

NKG2E_HUMAN

Accession

Primary (citable) accession number: Q07444
Secondary accession number(s): Q96RL0, Q9UP04

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 5, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)