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Protein

Bromodomain-containing factor 2

Gene

BDF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the expression of a broad class of genes including snRNAs. Required for sporulation and DNA-damage repair. Prevents the spreading of SIR silencing at telomeres and protects histone H4, but not H3, from deacetylation (By similarity).By similarity1 Publication

GO - Molecular functioni

  • core promoter binding Source: SGD
  • histone binding Source: SGD
  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29482-MONOMER.
ReactomeiR-SCE-3371568. Attenuation phase.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing factor 2
Gene namesi
Name:BDF2
Ordered Locus Names:YDL070W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL070W.
SGDiS000002228. BDF2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 638638Bromodomain-containing factor 2PRO_0000239629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei264 – 2641PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by the casein kinase CK2 complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07442.

PTM databases

iPTMnetiQ07442.

Interactioni

Subunit structurei

Interacts with the TFIID subunit TAF7 and with histone H4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358175EBI-37620,EBI-3493

GO - Molecular functioni

  • histone binding Source: SGD
  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi31990. 80 interactions.
DIPiDIP-1337N.
IntActiQ07442. 10 interactions.
MINTiMINT-410894.

Structurei

3D structure databases

ProteinModelPortaliQ07442.
SMRiQ07442. Positions 119-240, 319-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 22273Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini337 – 40973Bromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini506 – 59085NETPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili468 – 53770Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiQ07442.
KOiK11684.
OMAiATIARIY.
OrthoDBiEOG7Z69S1.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRTNMDTRH AHSALLAAPQ SATANSRSSN SSSESSSNKN NINVGVGDDS
60 70 80 90 100
GNVSAVSIDD GPHFRDIFHY GHEENYKLAS SGITNLNSSS HAHQTLSPIS
110 120 130 140 150
ISNASTPESF PEHPLGLERE TEPALEAEME AEELPPHQSK YLLSSIKATK
160 170 180 190 200
RLKDARPFLK PVDPIALNIP HYFNYVQTPM DLSLIETKLQ GNVYHSVEQV
210 220 230 240 250
TSDFKTMVDN CLNFNGPESS ISSMAKRIQK YFEKKLSAMP PRVLPASALK
260 270 280 290 300
KTSRNRKKNE DMDSPLVIRR SVSTTNDNIG ESGNREGVSG GRPKRTIHPP
310 320 330 340 350
KSKDLFDIYE NSKPKSKTLQ KKFRTCLKIL KVLMSKKNSD INFPFLQPVD
360 370 380 390 400
PIALNLPNYF DVVKNPMDLG TISNNLMNWK YKTIDQFVDD LNLVFYNCFQ
410 420 430 440 450
FNPEGNEVHS MGKKLKELFN FHWLENQDIL NEIETDSDLE EDNYSSSYSS
460 470 480 490 500
DDEYDDEDIN ENDITNPAIQ YLEQKLKKME VELQQLKRQE LSKLSKERKR
510 520 530 540 550
KHLGKTLLRR KAMKHSVDDL KKSITDKINE LSDLEMNGMI RIIKNSLPAD
560 570 580 590 600
EILTSNEDEI EIDLDILDEA TIARIYERYF EKKNNNNSKR KLSGNYSTAP
610 620 630
TNKKKKTLKF LEKDEIINNN NYSDSEEDSS DSSDSDSD
Length:638
Mass (Da):72,513
Last modified:November 1, 1996 - v1
Checksum:iA85CC497190345AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74119 Genomic DNA. Translation: CAA98636.1.
BK006938 Genomic DNA. Translation: DAA11788.1.
PIRiS67605.
RefSeqiNP_010213.1. NM_001180129.1.

Genome annotation databases

EnsemblFungiiYDL070W; YDL070W; YDL070W.
GeneIDi851488.
KEGGisce:YDL070W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74119 Genomic DNA. Translation: CAA98636.1.
BK006938 Genomic DNA. Translation: DAA11788.1.
PIRiS67605.
RefSeqiNP_010213.1. NM_001180129.1.

3D structure databases

ProteinModelPortaliQ07442.
SMRiQ07442. Positions 119-240, 319-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31990. 80 interactions.
DIPiDIP-1337N.
IntActiQ07442. 10 interactions.
MINTiMINT-410894.

PTM databases

iPTMnetiQ07442.

Proteomic databases

MaxQBiQ07442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL070W; YDL070W; YDL070W.
GeneIDi851488.
KEGGisce:YDL070W.

Organism-specific databases

EuPathDBiFungiDB:YDL070W.
SGDiS000002228. BDF2.

Phylogenomic databases

GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiQ07442.
KOiK11684.
OMAiATIARIY.
OrthoDBiEOG7Z69S1.

Enzyme and pathway databases

BioCyciYEAST:G3O-29482-MONOMER.
ReactomeiR-SCE-3371568. Attenuation phase.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

NextBioi968816.
PROiQ07442.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: DOMAIN BROMODOMAIN.
  4. "Bromodomain factor 1 corresponds to a missing piece of yeast TFIID."
    Matangkasombut O., Buratowski R.M., Swilling N.W., Buratowski S.
    Genes Dev. 14:951-962(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAF7.
  5. "Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation."
    Matangkasombut O., Buratowski S.
    Mol. Cell 11:353-363(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONE H4.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2."
    Sawa C., Nedea E., Krogan N., Wada T., Handa H., Greenblatt J., Buratowski S.
    Mol. Cell. Biol. 24:4734-4742(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY THE CK2 PROTEIN KINASE COMPLEX.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBDF2_YEAST
AccessioniPrimary (citable) accession number: Q07442
Secondary accession number(s): D6VRS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.