Q07439 (HSP71_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock 70 kDa protein 1A/1B

Alternative name(s):
Heat shock 70 kDa protein 1/2
Short name=HSP70-1/HSP70-2
Short name=HSP70.1/HSP70.2

Gene names

Name:	Hspa1a
Synonyms:	Hsp70-1, Hspa1
AND
Name:	Hspa1b
Synonyms:	Hsp70-2, Hspa2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.
Subunit structure	Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IRAK1BP1, TSC2 and DNAAF2. Component of the CatSper complex By similarity. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed By similarity. Interacts with CHCHD3 By similarity. Interacts with TRIM5 (via B30.2/SPRY domain) By similarity.
Subcellular location	Cytoplasm By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs By similarity.
Tissue specificity	HSPA1B is testis-specific.
Induction	By heat shock.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from direct assay PubMed 11820796. Source: RGD mRNA catabolic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from direct assay PubMed 16397188. Source: RGD negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay PubMed 16397188. Source: RGD negative regulation of vasoconstriction Inferred from mutant phenotype PubMed 15381648. Source: RGD positive regulation of T cell mediated cytotoxicity Inferred from direct assay PubMed 10679071. Source: RGD response to mechanical stimulus Inferred from expression pattern PubMed 20809232. Source: RGD response to unfolded protein Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 16609151. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 16609151. Source: RGD cytosol Inferred from direct assay PubMed 16135962PubMed 16609151. Source: RGD nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB protein complex Inferred from direct assay PubMed 16135962. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Heat shock 70 kDa protein 1A/1B		PRO_0000078254
Amino acid modifications
	Modified residue	108	1	N6-acetyllysine By similarity
	Modified residue	246	1	N6-acetyllysine By similarity
	Modified residue	348	1	N6-acetyllysine By similarity
	Modified residue	631	1	Phosphoserine By similarity
	Modified residue	633	1	Phosphoserine By similarity
	Modified residue	636	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	71 – 72	2	KR → NG in CAA52328. Ref.3
	Sequence conflict	71 – 72	2	KR → NG in CAA53140. Ref.4
	Sequence conflict	110	1	E → K in CAA53140. Ref.4
	Sequence conflict	204	1	T → R in CAA53140. Ref.4
	Sequence conflict	227	1	H → D in AAA17441. Ref.1
	Sequence conflict	262 – 267	6	RLRTAC → PLADGV in CAA53140. Ref.4
	Sequence conflict	408	1	G → A in CAA52328. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q07439 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 2D745B1013F64E7B	FASTA	641	70,185
	10 20 30 40 50 60 MAKKTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA 70 80 90 100 110 120 LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVVNDGDK PKVQVNYKGE NRSFYPEEIS 130 140 150 160 170 180 SMVLTKMKEI AEAYLGHPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA 190 200 210 220 230 240 IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVSH 250 260 270 280 290 300 FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA 310 320 330 340 350 360 RFEELCSDLF RGTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN 370 380 390 400 410 420 KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI 430 440 450 460 470 480 PTKQTQTFTT YSDNQPGVLI QVYEGERAMT RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI 490 500 510 520 530 540 DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAERYKAEDE VQRERVAAKN 550 560 570 580 590 600 ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDSNTLAEKE EFVHKREELE 610 620 630 640 RVCNPIISGL YQGAGAPGAG GFGAQAPKGG SGSGPTIEEV D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of stress-inducible rat hsp70 in normal and injured rat brain." Longo F.M., Wang S., Narasimhan P., Zhang J.S., Chen J., Massa S.M., Sharp F.R. J. Neurosci. Res. 36:325-335(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Comparative analysis of the three major histocompatibility complex-linked heat shock protein 70 (Hsp70) genes of the rat." Walter L., Rauh F., Guenther E. Immunogenetics 40:325-330(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LEW.1W/GUN.
[3]	"Cloning, nucleotide sequence and expression of rat heat inducible hsp70 gene." Lisowska K., Krawczyk Z., Widlak W., Wolniczek P., Wisniewski J. Biochim. Biophys. Acta 1219:64-72(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"Isolation of a novel inducible rat heat shock protein (HSP70) gene and its expression during ischaemia/hypoxia and heat shock." Mestril R., Chi S.H., Sayen M.R., Dillmann W.H. Biochem. J. 298:561-569(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Spleen.
[5]	"The genomic sequence and comparative analysis of the rat major histocompatibility complex." Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H. Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16764 mRNA. Translation: AAA17441.1. X77208 Genomic DNA. Translation: CAA54423.1. X77207 Genomic DNA. Translation: CAA54422.1. X74271 Genomic DNA. Translation: CAA52328.1. X75357 Genomic DNA. Translation: CAA53140.1. BX883045 Genomic DNA. Translation: CAE83977.1. BX883045 Genomic DNA. Translation: CAE83978.1.
IPI	IPI00196751.
PIR	I54542.
RefSeq	NP_114177.2. NM_031971.2. NP_997669.1. NM_212504.1.
UniGene	Rn.1950. Rn.228581.
3D structure databases
ProteinModelPortal	Q07439.
SMR	Q07439. Positions 1-619.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-240978.
STRING	10116.ENSRNOP00000050605.
PTM databases
PhosphoSite	Q07439.
Proteomic databases
PaxDb	Q07439.
PRIDE	Q07439.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000049667; ENSRNOP00000050605; ENSRNOG00000045654. ENSRNOT00000061950; ENSRNOP00000067749; ENSRNOG00000050647.
GeneID	24472. 294254.
KEGG	rno:24472. rno:294254.
Organism-specific databases
CTD	3303. 3304.
RGD	1593284. Hspa1a. 2840. Hspa1b.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00550000074467.
HOGENOM	HOG000228135.
HOVERGEN	HBG051845.
InParanoid	Q07439.
KO	K03283.
OMA	PIIEVEF.
OrthoDB	EOG4W6NVK.
Gene expression databases
ArrayExpress	Q07439.
Genevestigator	Q07439.
GermOnline	ENSRNOG00000033526. Rattus norvegicus.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603417.

Entry information

Entry name

HSP71_RAT

Accession

Primary (citable) accession number: Q07439
Secondary accession number(s): P42853, Q63256

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program