Reviewed, UniProtKB/Swiss-Prot Q07436 (EXPA_DROME)

Names and origin

Protein names

Recommended name:
    Protein expanded

Gene names

Name:	ex
ORF Names:	CG4114

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the control of cell proliferation in imaginal disks. May bind to certain proteins of signal transduction pathways by interaction with their SH3 domains.
Subcellular location	Apical cell membrane. Note: Apical surface of disk cells.
Disruption phenotype	Hyperplasmia of the imaginal disk resulting in wing overgrowth. This overgrowth is limited to specific regions along the 2 wing axs. Defects also in eyes, head, thorax and limbs where duplication and bulging often occur. Ref.1
Sequence similarities	Contains 1 FERM domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	SH3-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	compound eye photoreceptor cell differentiation Inferred from mutant phenotype. Source: FlyBase decapentaplegic receptor signaling pathway Inferred from genetic interaction. Source: FlyBase endocytic recycling Inferred from genetic interaction. Source: FlyBase negative regulation of cell proliferation Inferred from mutant phenotype. Source: FlyBase negative regulation of imaginal disc growth Inferred from mutant phenotype. Source: FlyBase regulation of cell differentiation Inferred from genetic interaction. Source: FlyBase regulation of exit from mitosis Inferred from mutant phenotype. Source: FlyBase regulation of signal transduction Inferred from genetic interaction. Source: FlyBase
Cellular component	apical plasma membrane Inferred from direct assay. Source: FlyBase cytoskeleton Inferred from electronic annotation. Source: InterPro zonula adherens Traceable author statement. Source: FlyBase
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1427	1427	Protein expanded		PRO_0000219443
Regions
	Domain	26 – 399	374	FERM
	Motif	1008 – 1016	9	SH3-binding Potential
	Motif	1012 – 1020	9	SH3-binding Potential
	Motif	1149 – 1157	9	SH3-binding Potential
	Compositional bias	409 – 412	4	Poly-Glu
	Compositional bias	782 – 788	7	Poly-Pro
	Compositional bias	952 – 955	4	Poly-His
	Compositional bias	1002 – 1005	4	Poly-Pro
	Compositional bias	1011 – 1017	7	Poly-Pro
	Compositional bias	1081 – 1084	4	Poly-Pro
	Compositional bias	1149 – 1154	6	Poly-Pro
	Compositional bias	1158 – 1168	11	Poly-Ala
	Compositional bias	1170 – 1174	5	Poly-Ser
	Compositional bias	1199 – 1205	7	Poly-Pro
	Compositional bias	1416 – 1422	7	Poly-Gln
Amino acid modifications
	Modified residue	1181	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	160 – 161	2	EQ → DE in AAB39774. Ref.1
	Sequence conflict	194	1	S → L in AAB39774. Ref.1
	Sequence conflict	347	1	F → S in AAB39774. Ref.1
	Sequence conflict	563	1	I → T in AAB39774. Ref.1
	Sequence conflict	671	1	A → D in AAB39774. Ref.1
	Sequence conflict	693	1	Q → S in AAB39774. Ref.1
	Sequence conflict	1293 – 1294	2	SM → FN in AAB39774. Ref.1
	Sequence conflict	1422	1	Q → QQQ in AAB39774. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07436-1 [UniParc]. Last modified June 21, 2005. Version 3. Checksum: 3CC1AD0B4D900EB2	FASTA	1,427	153,644
	10 20 30 40 50 60 MRAFCTVSAP LEVCASSAEQ LSPGSRFLAL RLLGQQQPKT LYFLVDAKSR VREVYTQTCL 70 80 90 100 110 120 HFATQGMLDT ELFGLAVLID GEYMFADPES KLSKYGPKSW RSSHTHGLDA NGRPLLELHF 130 140 150 160 170 180 RVQFYIESPF MLKDETSRHN YYLQLRHNIL QRDLPREQAE QALVFLAGLA LQADLGDAPP 190 200 210 220 230 240 GTSNSKDDSG EETSASPSNG GRGLSATTTL PKISKRANER MLRLSTYVAS TSKRETIPLP 250 260 270 280 290 300 PSLPPNGADY YRIEDYLPSG LHTPWARSAM RACHREHLGM ATAEAELLYI QQACSLHETI 310 320 330 340 350 360 NAHTYRMRLA KSEQGSGSAW FVVYAKGIKI LGGESTNSSS NPETTTFLWP NITKLSFERK 370 380 390 400 410 420 KFEIRSGESR ITLYAASDEK NKLLLTLCKD THQWSMKLAA RLKEVSKREE EEAAESQRLH 430 440 450 460 470 480 ASYACSRSLL LPYKSKNEQR ISVISSTSSN TTSGIVSDRV HSEDELEIMI NTPPAPLAAP 490 500 510 520 530 540 STESLALAHL LDRPSVSRQT SSVGQMSLKD LEEQLAALSV RPQDASSNGA TIVTNSSVQR 550 560 570 580 590 600 NSMGTTANDS STATDSPSSQ HNIGSQCSST CSTVVVTSPV NGAGASSSGA PIPVHSTSSS 610 620 630 640 650 660 LELGFSHTAQ NSALSETSPD DFLSTSAREE TESVSGASGV YTLAHGAPPT ETSGVYTMHS 670 680 690 700 710 720 SELTGQSSEI AESEKSSHYG MFQPQKLEET HVQHSDSVDG KKKEDFRPRS DSNVSTGSSF 730 740 750 760 770 780 RGDGSDPTDN KHSLLSAEEL TNLIVGRGTY PSRKTVSSSL HSDCDYVTLP LGDQGEEEVD 790 800 810 820 830 840 QPPAPPPPYS ARHEKTGLCG PPIAKPIPKP IAVVAPKPDS PPCSPPVPPA PIPAPPPAIR 850 860 870 880 890 900 RRDPPPYSIS SKPRPTSLIS VSSSAHPAPS AAGSMSSLKS EEVTARFITT RPQISILKAH 910 920 930 940 950 960 TSLIPDGAKP SYAAPHHCSS VASSNGSVCS HQLSQQSLHN SNYAGGSQAS LHHHHVPSHH 970 980 990 1000 1010 1020 RHSGSAAIGI VPYGLHKSTA SLHHQQSCVL LPVIKPRQFL APPPPSLPRQ PPPPPPPNHP 1030 1040 1050 1060 1070 1080 HLASHLYERE MARKQLELYQ QQLYSDVDYV IYPIQDPAVS QQEYLDAKQG SLLAAMAQAA 1090 1100 1110 1120 1130 1140 PPPPHHPYLA MQVSPAIYRS TPYLPLTLST HSRYASTQNL SDTYVQLPGP GYSPLYSPSM 1150 1160 1170 1180 1190 1200 ASLCSSYEPP PPPPLHPAAL AAAAAAGAGS SSSSMFARSR SDDNILNSLD LLPKGKRLPP 1210 1220 1230 1240 1250 1260 PPPPPYVNRR LKKPPMPAPS EKPPPIPSKP IPSRMSPIPP RKPPTLNPHH ANSPLTKTSS 1270 1280 1290 1300 1310 1320 GAQWAGERPR PDLGLGLGLN RGNNSILAQL QASMVAQSHA QAQAQALDIA LLREKSKHLD 1330 1340 1350 1360 1370 1380 LPLISALCND RSLLKQTKVV INPKTGQEMP TSSAQPSGAT TNGVANSSAG AGTLSKARKG 1390 1400 1410 1420 STVSHRHPQD KLPPLPVQQL AEANNYVIDP AVMMKQQQQQ QQHNKTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expanded: a gene involved in the control of cell proliferation in imaginal discs." Boedigheimer M., Laughon A. Development 118:1291-1301(1993) [PubMed: 8269855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE. Tissue: Imaginal disk.
[2]	Boedigheimer M. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1181, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L14768 mRNA. Translation: AAB39774.1. AE014134 Genomic DNA. Translation: AAF51495.1. AY069068 mRNA. Translation: AAL39213.1.
PIR	T13720.
RefSeq	NP_476840.2.
UniGene	Dm.247
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07436. 2 interactions.
Genome annotation databases
Ensembl	FBgn0004583. Drosophila melanogaster. [Contig view]
GeneID	33218.
KEGG	dme:Dmel_CG4114.
NMPDR	fig|7227.3.peg.98.
Organism-specific databases
FlyBase	FBgn0004583. ex.
Phylogenomic databases
HOGENOM	Q07436.
OMA	Q07436. QRISVIS.
Gene expression databases
ArrayExpress	Q07436.
GermOnline	CG4114. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR014352. FERM/acyl-CoA_bd_prot_3-hlx. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR018979. FERM_N. IPR018980. FERM_PH-like_C. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:1.20.80.10. ACBP. 1 hit. G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF09380. FERM_C. 1 hit. PF00373. FERM_M. 1 hit. PF09379. FERM_N. 1 hit. [Graphical view]
SMART	SM00295. B41. 1 hit. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	782491.

Entry information

Entry name

EXPA_DROME

Accession

Primary (citable) accession number: Q07436
Secondary accession number(s): Q9VPQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project