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Protein

Nitrogen regulatory PII-like protein

Gene

nrgB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for full induction of the nrgAB operon under conditions of ammonium limitation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciBSUB:BSU36520-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory PII-like protein
Gene namesi
Name:nrgB
Ordered Locus Names:BSU36520
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells show no growth phenotype even at acidic pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Nitrogen regulatory PII-like proteinPRO_0000139814Add
BLAST

Proteomic databases

PaxDbiQ07428.

Expressioni

Inductioni

The nrgAB operon is activated by TnrA under nitrogen-limited conditions.1 Publication

Interactioni

Subunit structurei

Needs to interact with NrgA in order to localize correctly to the membrane.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019741.

Structurei

Secondary structure

1
116
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi17 – 2711Combined sources
Beta strandi33 – 4614Combined sources
Helixi48 – 503Combined sources
Beta strandi53 – 7220Combined sources
Helixi74 – 8512Combined sources
Beta strandi88 – 903Combined sources
Beta strandi94 – 996Combined sources
Beta strandi101 – 1055Combined sources
Turni106 – 1083Combined sources
Helixi112 – 1143Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R25X-ray2.52A5-116[»]
4RX6X-ray2.60A/B/D1-116[»]
ProteinModelPortaliQ07428.
SMRiQ07428. Positions 5-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiQ07428.
KOiK04751.
OMAiGGHKEVY.
PhylomeDBiQ07428.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQMFKVEI VTRPANFEKL KQELGKIGVT SLTFSNVHGC GLQKAHTELY
60 70 80 90 100
RGVKIESNVY ERLKIEIVVS KVPVDQVTET AKRVLKTGSP GDGKIFVYEI
110
SNTINIRTGE EGPEAL
Length:116
Mass (Da):12,822
Last modified:October 1, 1994 - v1
Checksum:i386FCB5C1133CC59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03216 Unassigned DNA. Translation: AAA17400.1.
Z82987 Genomic DNA. Translation: CAB05373.1.
AL009126 Genomic DNA. Translation: CAB15669.1.
PIRiB36865.
RefSeqiNP_391533.1. NC_000964.3.
WP_003221829.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15669; CAB15669; BSU36520.
GeneIDi11241258.
936937.
KEGGibsu:BSU36520.
PATRICi18979328. VBIBacSub10457_3826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03216 Unassigned DNA. Translation: AAA17400.1.
Z82987 Genomic DNA. Translation: CAB05373.1.
AL009126 Genomic DNA. Translation: CAB15669.1.
PIRiB36865.
RefSeqiNP_391533.1. NC_000964.3.
WP_003221829.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R25X-ray2.52A5-116[»]
4RX6X-ray2.60A/B/D1-116[»]
ProteinModelPortaliQ07428.
SMRiQ07428. Positions 5-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019741.

Proteomic databases

PaxDbiQ07428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15669; CAB15669; BSU36520.
GeneIDi11241258.
936937.
KEGGibsu:BSU36520.
PATRICi18979328. VBIBacSub10457_3826.

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiQ07428.
KOiK04751.
OMAiGGHKEVY.
PhylomeDBiQ07428.

Enzyme and pathway databases

BioCyciBSUB:BSU36520-MONOMER.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRGB_BACSU
AccessioniPrimary (citable) accession number: Q07428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.