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Protein

Nitrogen regulatory PII-like protein

Gene

nrgB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for full induction of the nrgAB operon under conditions of ammonium limitation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciBSUB:BSU36520-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory PII-like protein
Gene namesi
Name:nrgB
Ordered Locus Names:BSU36520
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells show no growth phenotype even at acidic pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Nitrogen regulatory PII-like proteinPRO_0000139814Add
BLAST

Proteomic databases

PaxDbiQ07428.

Expressioni

Inductioni

The nrgAB operon is activated by TnrA under nitrogen-limited conditions.1 Publication

Interactioni

Subunit structurei

Needs to interact with NrgA in order to localize correctly to the membrane.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019741.

Structurei

Secondary structure

1
116
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi17 – 2711Combined sources
Beta strandi33 – 4614Combined sources
Helixi48 – 503Combined sources
Beta strandi53 – 7220Combined sources
Helixi74 – 8512Combined sources
Beta strandi88 – 903Combined sources
Beta strandi94 – 996Combined sources
Beta strandi101 – 1055Combined sources
Turni106 – 1083Combined sources
Helixi112 – 1143Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R25X-ray2.52A5-116[»]
4RX6X-ray2.60A/B/D1-116[»]
ProteinModelPortaliQ07428.
SMRiQ07428. Positions 5-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiQ07428.
KOiK04751.
OMAiEMVEPAI.
OrthoDBiEOG69GZGV.
PhylomeDBiQ07428.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQMFKVEI VTRPANFEKL KQELGKIGVT SLTFSNVHGC GLQKAHTELY
60 70 80 90 100
RGVKIESNVY ERLKIEIVVS KVPVDQVTET AKRVLKTGSP GDGKIFVYEI
110
SNTINIRTGE EGPEAL
Length:116
Mass (Da):12,822
Last modified:October 1, 1994 - v1
Checksum:i386FCB5C1133CC59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03216 Unassigned DNA. Translation: AAA17400.1.
Z82987 Genomic DNA. Translation: CAB05373.1.
AL009126 Genomic DNA. Translation: CAB15669.1.
PIRiB36865.
RefSeqiNP_391533.1. NC_000964.3.
WP_003221829.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15669; CAB15669; BSU36520.
GeneIDi11241258.
936937.
KEGGibsu:BSU36520.
PATRICi18979328. VBIBacSub10457_3826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03216 Unassigned DNA. Translation: AAA17400.1.
Z82987 Genomic DNA. Translation: CAB05373.1.
AL009126 Genomic DNA. Translation: CAB15669.1.
PIRiB36865.
RefSeqiNP_391533.1. NC_000964.3.
WP_003221829.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R25X-ray2.52A5-116[»]
4RX6X-ray2.60A/B/D1-116[»]
ProteinModelPortaliQ07428.
SMRiQ07428. Positions 5-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019741.

Proteomic databases

PaxDbiQ07428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15669; CAB15669; BSU36520.
GeneIDi11241258.
936937.
KEGGibsu:BSU36520.
PATRICi18979328. VBIBacSub10457_3826.

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiQ07428.
KOiK04751.
OMAiEMVEPAI.
OrthoDBiEOG69GZGV.
PhylomeDBiQ07428.

Enzyme and pathway databases

BioCyciBSUB:BSU36520-MONOMER.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein."
    Wray L.V. Jr., Atkinson M.R., Fisher S.H.
    J. Bacteriol. 176:108-114(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB."
    Detsch C., Stuelke J.
    Microbiology 149:3289-3297(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
    Strain: 168.
  5. "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box."
    Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.
    Mol. Microbiol. 49:157-165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY TNRA.
  6. "Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor."
    Wray L.V. Jr., Zalieckas J.M., Fisher S.H.
    J. Mol. Biol. 300:29-40(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION OF THE NRGAB OPERON BY TNRA.
    Strain: 168.

Entry informationi

Entry nameiNRGB_BACSU
AccessioniPrimary (citable) accession number: Q07428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.