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Q07422

- DRTS_TOXGO

UniProt

Q07422 - DRTS_TOXGO

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene
N/A
Organism
Toxoplasma gondii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81Substrate; via carbonyl oxygenBy similarity
    Binding sitei10 – 101NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei31 – 311SubstrateBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei172 – 1721SubstrateBy similarity
    Binding sitei344 – 3441dUMPBy similarity
    Active sitei489 – 4891By similarity
    Binding sitei490 – 4901dUMPBy similarity
    Binding sitei521 – 5211dUMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 227NADPBy similarity
    Nucleotide bindingi81 – 833NADPBy similarity
    Nucleotide bindingi102 – 1054NADPBy similarity
    Nucleotide bindingi152 – 1598NADPBy similarity
    Nucleotide bindingi509 – 5135dUMPBy similarity
    Nucleotide bindingi551 – 5533dUMPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. glycine biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
    Short name:
    DHFR-TS
    Including the following 2 domains:
    Dihydrofolate reductase (EC:1.5.1.3)
    Thymidylate synthase (EC:2.1.1.45)
    OrganismiToxoplasma gondii
    Taxonomic identifieri5811 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 610610Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186352Add
    BLAST

    Interactioni

    Structurei

    Secondary structure

    1
    610
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 117
    Beta strandi16 – 194
    Beta strandi24 – 263
    Helixi29 – 4012
    Beta strandi74 – 807
    Helixi81 – 866
    Helixi89 – 913
    Beta strandi97 – 1026
    Helixi108 – 1147
    Beta strandi125 – 1295
    Helixi130 – 14112
    Turni142 – 1443
    Beta strandi145 – 1506
    Helixi154 – 16310
    Beta strandi166 – 1749
    Beta strandi181 – 1833
    Helixi190 – 1923
    Beta strandi227 – 2326
    Beta strandi236 – 2383
    Beta strandi241 – 25010
    Turni288 – 2903
    Helixi291 – 2977
    Helixi325 – 33713
    Beta strandi339 – 3413
    Beta strandi349 – 36012
    Beta strandi362 – 3643
    Beta strandi369 – 3713
    Helixi375 – 38612
    Helixi393 – 3964
    Turni397 – 3993
    Helixi404 – 4063
    Helixi409 – 4146
    Helixi429 – 4357
    Helixi454 – 46411
    Beta strandi472 – 4743
    Turni478 – 4803
    Helixi481 – 4833
    Beta strandi484 – 4863
    Beta strandi489 – 4979
    Beta strandi501 – 51313
    Turni514 – 5163
    Helixi517 – 53519
    Beta strandi539 – 55315
    Helixi554 – 5563
    Helixi557 – 5637
    Beta strandi573 – 5775
    Helixi579 – 5813
    Helixi585 – 5873
    Beta strandi592 – 5976

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4ECKX-ray3.52A/B1-610[»]
    4EILX-ray2.20A/B/C/D/E/F/G/H1-610[»]
    ProteinModelPortaliQ07422.
    SMRiQ07422. Positions 322-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 250247DHFRAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni322 – 610289Thymidylate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
    In the C-terminal section; belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    OMAiLTCMIAQ.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    3.40.430.10. 2 hits.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000389. DHFR-TS. 1 hit.
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF53597. SSF53597. 2 hits.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKPVCLVVA MTPKRGIGIN NGLPWPHLTT DFKHFSRVTK TTPEEASRLN    50
    GWLPRKFAKT GDSGLPSPSV GKRFNAVVMG RKTWESMPRK FRPLVDRLNI 100
    VVSSSLKEED IAAEKPQAEG QQRVRVCASL PAALSLLEEE YKDSVDQIFV 150
    VGGAGLYEAA LSLGVASHLY ITRVAREFPC DVFFPAFPGD DILSNKSTAA 200
    QAAAPAESVF VPFCPELGRE KDNEATYRPI FISKTFSDNG VPYDFVVLEK 250
    RRKTDDAATA EPSNAMSSLT STRETTPVHG LQAPSSAAAI APVLAWMDEE 300
    DRKKREQKEL IRAVPHVHFR GHEEFQYLDL IADIINNGRT MDDRTGVGVI 350
    SKFGCTMRYS LDQAFPLLTT KRVFWKGVLE ELLWFIRGDT NANHLSEKGV 400
    KIWDKNVTRE FLDSRNLPHR EVGDIGPGYG FQWRHFGAAY KDMHTDYTGQ 450
    GVDQLKNVIQ MLRTNPTDRR MLMTAWNPAA LDEMALPPCH LLCQFYVNDQ 500
    KELSCIMYQR SCDVGLGVPF NIASYSLLTL MVAHVCNLKP KEFIHFMGNT 550
    HVYTNHVEAL KEQLRREPRP FPIVNILNKE RIKEIDDFTA EDFEVVGYVP 600
    HGRIQMEMAV 610
    Length:610
    Mass (Da):68,752
    Last modified:February 1, 1995 - v1
    Checksum:iCFAF564416D04A29
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08489 Genomic DNA. Translation: AAB00163.1.
    PIRiA46005.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08489 Genomic DNA. Translation: AAB00163.1 .
    PIRi A46005.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4ECK X-ray 3.52 A/B 1-610 [» ]
    4EIL X-ray 2.20 A/B/C/D/E/F/G/H 1-610 [» ]
    ProteinModelPortali Q07422.
    SMRi Q07422. Positions 322-610.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q07422.
    ChEMBLi CHEMBL2425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OMAi LTCMIAQ.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    3.40.430.10. 2 hits.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000389. DHFR-TS. 1 hit.
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF53597. SSF53597. 2 hits.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the dihydrofolate reductase-thymidylate synthase gene from Toxoplasma gondii."
      Roos D.S.
      J. Biol. Chem. 268:6269-6280(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RH.

    Entry informationi

    Entry nameiDRTS_TOXGO
    AccessioniPrimary (citable) accession number: Q07422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3