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Q07412

- TPIS_PLAFA

UniProt

Q07412 - TPIS_PLAFA

Protein

Triosephosphate isomerase

Gene

TPI

Organism
Plasmodium falciparum
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glyceraldehyde 3-phosphate = glycerone phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Substrate
    Binding sitei12 – 121Substrate
    Active sitei95 – 951Electrophile
    Active sitei165 – 1651Proton acceptor

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. triose-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. glycolytic process Source: UniProtKB-UniPathway
    3. pentose-phosphate shunt Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis, Pentose shunt

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00189.
    UPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triosephosphate isomerase (EC:5.3.1.1)
    Short name:
    TIM
    Alternative name(s):
    Triose-phosphate isomerase
    Gene namesi
    Name:TPI
    OrganismiPlasmodium falciparum
    Taxonomic identifieri5833 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Triosephosphate isomerasePRO_0000090138Add
    BLAST

    Proteomic databases

    PRIDEiQ07412.

    Interactioni

    Subunit structurei

    Homodimer.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-7086711,EBI-7086711

    Protein-protein interaction databases

    MINTiMINT-7137584.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 106
    Helixi17 – 2812
    Turni34 – 363
    Beta strandi37 – 426
    Helixi45 – 473
    Helixi48 – 547
    Beta strandi59 – 646
    Beta strandi71 – 733
    Helixi80 – 856
    Beta strandi90 – 934
    Helixi96 – 1016
    Helixi106 – 11813
    Beta strandi122 – 1276
    Helixi131 – 1355
    Helixi139 – 14810
    Helixi151 – 1533
    Beta strandi159 – 1646
    Helixi167 – 1693
    Beta strandi170 – 1734
    Helixi178 – 19518
    Helixi198 – 2036
    Beta strandi204 – 2085
    Turni214 – 2163
    Helixi217 – 2215
    Beta strandi228 – 2314
    Helixi233 – 2364
    Helixi240 – 2467

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LYXX-ray1.90A1-248[»]
    1LZOX-ray2.80A/B/C/D1-248[»]
    1M7OX-ray2.40A/B1-248[»]
    1M7PX-ray2.40A/B1-248[»]
    1O5XX-ray1.10A/B1-248[»]
    1VGAX-ray1.80A/B/C/D1-248[»]
    1WOAX-ray2.80A/B/C/D1-248[»]
    1WOBX-ray2.80A/B/C/D1-248[»]
    1YDVX-ray2.20A/B1-248[»]
    2FI6model-A1-248[»]
    2VFDX-ray1.40A/B1-248[»]
    2VFEX-ray2.20A/B1-248[»]
    2VFFX-ray1.70A/B1-248[»]
    2VFGX-ray1.95A/B/C/D1-248[»]
    2VFHX-ray2.00A/B1-248[»]
    2VFIX-ray2.25A/B1-248[»]
    3PSVX-ray2.00A/B1-248[»]
    3PSWX-ray1.99A/B1-248[»]
    3PVFX-ray1.73A1-248[»]
    3PWAX-ray2.04A/B1-248[»]
    3PY2X-ray1.93A/B1-248[»]
    DisProtiDP00614.
    ProteinModelPortaliQ07412.
    SMRiQ07412. Positions 3-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ07412.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the triosephosphate isomerase family.Curated

    Phylogenomic databases

    eggNOGiCOG0149.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00147_B. TIM_B.
    InterProiIPR013785. Aldolase_TIM.
    IPR022896. TrioseP_Isoase_bac/euk.
    IPR000652. Triosephosphate_isomerase.
    IPR020861. Triosephosphate_isomerase_AS.
    [Graphical view]
    PANTHERiPTHR21139. PTHR21139. 1 hit.
    PfamiPF00121. TIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF51351. SSF51351. 1 hit.
    TIGRFAMsiTIGR00419. tim. 1 hit.
    PROSITEiPS00171. TIM_1. 1 hit.
    PS51440. TIM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07412-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARKYFVAAN WKCNGTLESI KSLTNSFNNL DFDPSKLDVV VFPVSVHYDH    50
    TRKLLQSKFS TGIQNVSKFG NGSYTGEVSA EIAKDLNIEY VIIGHFERRK 100
    YFHETDEDVR EKLQASLKNN LKAVVCFGES LEQREQNKTI EVITKQVKAF 150
    VDLIDNFDNV ILAYEPLWAI GTGKTATPEQ AQLVHKEIRK IVKDTCGEKQ 200
    ANQIRILYGG SVNTENCSSL IQQEDIDGFL VGNASLKESF VDIIKSAM 248
    Length:248
    Mass (Da):27,935
    Last modified:October 1, 1994 - v1
    Checksum:iEFC8A4373EA9BED1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01654 mRNA. Translation: AAA18799.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01654 mRNA. Translation: AAA18799.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LYX X-ray 1.90 A 1-248 [» ]
    1LZO X-ray 2.80 A/B/C/D 1-248 [» ]
    1M7O X-ray 2.40 A/B 1-248 [» ]
    1M7P X-ray 2.40 A/B 1-248 [» ]
    1O5X X-ray 1.10 A/B 1-248 [» ]
    1VGA X-ray 1.80 A/B/C/D 1-248 [» ]
    1WOA X-ray 2.80 A/B/C/D 1-248 [» ]
    1WOB X-ray 2.80 A/B/C/D 1-248 [» ]
    1YDV X-ray 2.20 A/B 1-248 [» ]
    2FI6 model - A 1-248 [» ]
    2VFD X-ray 1.40 A/B 1-248 [» ]
    2VFE X-ray 2.20 A/B 1-248 [» ]
    2VFF X-ray 1.70 A/B 1-248 [» ]
    2VFG X-ray 1.95 A/B/C/D 1-248 [» ]
    2VFH X-ray 2.00 A/B 1-248 [» ]
    2VFI X-ray 2.25 A/B 1-248 [» ]
    3PSV X-ray 2.00 A/B 1-248 [» ]
    3PSW X-ray 1.99 A/B 1-248 [» ]
    3PVF X-ray 1.73 A 1-248 [» ]
    3PWA X-ray 2.04 A/B 1-248 [» ]
    3PY2 X-ray 1.93 A/B 1-248 [» ]
    DisProti DP00614.
    ProteinModelPortali Q07412.
    SMRi Q07412. Positions 3-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-7137584.

    Proteomic databases

    PRIDEi Q07412.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0149.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00189 .
    UPA00138 .

    Miscellaneous databases

    EvolutionaryTracei Q07412.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00147_B. TIM_B.
    InterProi IPR013785. Aldolase_TIM.
    IPR022896. TrioseP_Isoase_bac/euk.
    IPR000652. Triosephosphate_isomerase.
    IPR020861. Triosephosphate_isomerase_AS.
    [Graphical view ]
    PANTHERi PTHR21139. PTHR21139. 1 hit.
    Pfami PF00121. TIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51351. SSF51351. 1 hit.
    TIGRFAMsi TIGR00419. tim. 1 hit.
    PROSITEi PS00171. TIM_1. 1 hit.
    PS51440. TIM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the triosephosphate isomerase gene of Plasmodium falciparum and expression in Escherichia coli."
      Ranie J., Kumar V.P., Balaram H.
      Mol. Biochem. Parasitol. 61:159-169(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Palo Alto.
    2. "Triosephosphate isomerase from Plasmodium falciparum: the crystal structure provides insights into antimalarial drug design."
      Velanker S.S., Ray S.S., Gokhale R.S., Suma S., Balaram H., Balaram P., Murthy M.R.N.
      Structure 5:751-761(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), HOMODIMERIZATION.
    3. "Structures of Plasmodium falciparum triosephosphate isomerase complexed to substrate analogues: observation of the catalytic loop in the open conformation in the ligand-bound state."
      Parthasarathy S., Balaram H., Balaram P., Murthy M.R.N.
      Acta Crystallogr. D 58:1992-2000(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS, HOMODIMERIZATION.
    4. "Structure of the Plasmodium falciparum triosephosphate isomerase-phosphoglycolate complex in two crystal forms: characterization of catalytic loop open and closed conformations in the ligand-bound state."
      Parthasarathy S., Ravindra G., Balaram H., Balaram P., Murthy M.R.N.
      Biochemistry 41:13178-13188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION.
    5. "Structure of Plasmodium falciparum triose-phosphate isomerase-2-phosphoglycerate complex at 1.1-A resolution."
      Parthasarathy S., Eaazhisai K., Balaram H., Balaram P., Murthy M.R.N.
      J. Biol. Chem. 278:52461-52470(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG.
    6. "Structures of unliganded and inhibitor complexes of W168F, a Loop6 hinge mutant of Plasmodium falciparum triosephosphate isomerase: observation of an intermediate position of loop6."
      Eaazhisai K., Balaram H., Balaram P., Murthy M.R.N.
      J. Mol. Biol. 343:671-684(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG.

    Entry informationi

    Entry nameiTPIS_PLAFA
    AccessioniPrimary (citable) accession number: Q07412
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3