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Protein

Urease subunit alpha

Gene

ureC

Organism
Bacillus sp. (strain TB-90)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit gamma (ureA), Urease subunit alpha (ureC)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi138Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi219Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi219Nickel 2; via carbamate groupUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Metal bindingi248Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi274Nickel 2; via tele nitrogenUniRule annotation1
Active sitei322Proton donorUniRule annotation1
Metal bindingi362Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
OrganismiBacillus sp. (strain TB-90)
Taxonomic identifieri36824 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000675371 – 569Urease subunit alphaAdd BLAST569

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ07397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 569UreaseUniRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSMSRKQY ADMFGPTVGD AIRLADSELF IEIEKDYTTY GDEVKFGGGK
60 70 80 90 100
VIRDGMGQHP LATSDECVDL VLTNAIIVDY TGIYKADIGI KDGMIASIGK
110 120 130 140 150
AGNPLLMDGV DMVIGAATEV IAAEGMIVTA GGIDAHIHFI CPQQIETALA
160 170 180 190 200
SGVTTMIGGG TGPATGTNAT TCTPGPWNIH RMLQAAEEFP INLGFLGKGN
210 220 230 240 250
CSDEAPLKEQ IEAGAVGLKL HEDWGSTAAA IDTCLKVADR YDVQVAIHTD
260 270 280 290 300
TLNEGGFVED TLKAIDGRVI HTYHTEGAGG GHAPDIIKAA GFPNILPSST
310 320 330 340 350
NPTRPYTINT LEEHLDMLMV CHHLDANIPE DIAFADSRIR KETIAAEDVL
360 370 380 390 400
HDLGVFSMIS SDSQAMGRVG EVIIRTWQTA DKMKKQRGKL QEDNGVGDNF
410 420 430 440 450
RVKRYIAKYT INPAIAHGIA DYVGSVEVGK LADLVVWNPA FFGVKPELVL
460 470 480 490 500
KGGMIAYSTM GDPNASIPTP QPVLYRPMFA AKGDAKYQTS ITFVSKAAYE
510 520 530 540 550
KGIHEQLGLK KKVKPVHGIR KLTKKDLILN DKTPKIDVDP QTYEVKVDGQ
560
LVTCEPAEIV PMAQRYFLF
Length:569
Mass (Da):61,397
Last modified:October 1, 1994 - v1
Checksum:i38AA83133A31A49D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14439 Genomic DNA. Translation: BAA03325.1.
PIRiC36950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14439 Genomic DNA. Translation: BAA03325.1.
PIRiC36950.

3D structure databases

ProteinModelPortaliQ07397.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_BACSB
AccessioniPrimary (citable) accession number: Q07397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.