Reviewed, UniProtKB/Swiss-Prot Q07353 (FL3H_PETHY)

Names and origin

Protein names

Recommended name:
    Naringenin,2-oxoglutarate 3-dioxygenase
    EC=1.14.11.9
Alternative name(s):
    Flavanone-3-hydroxylase
      Short name=F3H
    FHT

Gene names

Name:

AN3

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	369 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 369	›369	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067289
Regions
	Domain	193 – 297	105	Fe2OG dioxygenase
Sites
	Metal binding	78	1	Iron Potential
	Metal binding	220	1	Iron Potential
	Metal binding	222	1	Iron Potential
	Metal binding	278	1	Iron Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q07353-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C34034E23B4D5A3F	FASTA	369	41,466
	10 20 30 40 50 60 IPRVTPSTLT ALAEEKTLQT SFIRDEDERP KVAYNQFSNE IPIISLEGID DETGKRAEIC 70 80 90 100 110 120 DKIVKACEDW GVFQVVDHGV DAEVISQMTT FAKEFFALPP EEKLRFDMSG GKKGGFIVSS 130 140 150 160 170 180 HLQGEVVQDW REIVTYFSYP TRARDYSRWP DKPEGWIAVT QKYSEKLMEL ACKLLDVLSE 190 200 210 220 230 240 AMGLEKEALT KACVDMDQKV VVNFYPKCPE PDLTLGLKRH TDPGTITLLL QDQVGGLQAT 250 260 270 280 290 300 KDNGKTWITV QPVEGAFVVN LGDHGHFLSN GRFKNADHQA VVNSNSSRLS IATFQNPAPE 310 320 330 340 350 360 AIVYPLKIRE GEKSIMDEPI TFAEMYRRKM SKDLELARLK KQAKEQQLQA EVAAEKAKLE SKPIEEILA

References

[1]

"Molecular cloning, sequence analysis, and in vitro expression of flavanone 3 beta-hydroxylase from Petunia hybrida." Britsch L., Ruhnau-Brich B., Forkmann G. J. Biol. Chem. 267:5380-5387(1992) [PubMed: 1544919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Blue Titan. Tissue: Flower bud.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60512 mRNA. Translation: CAA43027.1.
PIR	A42110. S16780.
3D structure databases
SMR	Q07353. Positions 6-332.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11838.
BRENDA	1.14.11.9. 2263.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_PETHY

Accession

Primary (citable) accession number: Q07353

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)