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Protein

Pre-mRNA-splicing factor PRP11

Gene

PRP11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri66 – 9631Matrin-typeAdd
BLAST

GO - Molecular functioni

  • RNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor PRP11
Gene namesi
Name:PRP11
Synonyms:PRP10, RNA11
Ordered Locus Names:YDL043C
ORF Names:D2711
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL043C.
SGDiS000002201. PRP11.

Subcellular locationi

GO - Cellular componenti

  • U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Pre-mRNA-splicing factor PRP11PRO_0000174317Add
BLAST

Proteomic databases

MaxQBiQ07350.
PeptideAtlasiQ07350.

PTM databases

iPTMnetiQ07350.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CUS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP21P325246EBI-688,EBI-603

Protein-protein interaction databases

BioGridi32016. 100 interactions.
DIPiDIP-1001N.
IntActiQ07350. 14 interactions.
MINTiMINT-383970.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi153 – 1586Combined sources
Beta strandi162 – 1643Combined sources
Beta strandi179 – 1846Combined sources
Beta strandi197 – 2026Combined sources
Beta strandi204 – 2063Combined sources
Helixi235 – 2395Combined sources
Beta strandi241 – 2444Combined sources
Turni245 – 2484Combined sources
Beta strandi249 – 2524Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DGWX-ray3.11C149-266[»]
ProteinModelPortaliQ07350.
SMRiQ07350. Positions 149-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 154Poly-Gly

Sequence similaritiesi

Belongs to the SF3A2 family.Curated
Contains 1 matrin-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri66 – 9631Matrin-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00720000108823.
HOGENOMiHOG000191688.
InParanoidiQ07350.
KOiK12826.
OMAiPCETIAI.
OrthoDBiEOG77HDS9.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD
60 70 80 90 100
QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKKH GLNVLRRGIS
110 120 130 140 150
IEKSSLGREG QTTHDFRQQQ KIIEAKQSLK NNGTIPVCKI ATVKNPKNGS
160 170 180 190 200
VGLAIQVNYS SEVKENSVDS DDKAKVPPLI RIVSGLELSD TKQKGKKFLV
210 220 230 240 250
IAYEPFENIA IELPPNEILF SENNDMDNNN DGVDELNKKC TFWDAISKLY
260
YVQFFFKQAE QEQADV
Length:266
Mass (Da):29,921
Last modified:November 1, 1997 - v1
Checksum:iD8BA3BB27F36F06F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71781 Genomic DNA. Translation: CAA96446.1. Sequence problems.
Z74091 Genomic DNA. Translation: CAA98602.1.
BK006938 Genomic DNA. Translation: DAA11813.1.
PIRiS67576.
RefSeqiNP_010241.1. NM_001180102.1.

Genome annotation databases

EnsemblFungiiYDL043C; YDL043C; YDL043C.
GeneIDi851518.
KEGGisce:YDL043C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71781 Genomic DNA. Translation: CAA96446.1. Sequence problems.
Z74091 Genomic DNA. Translation: CAA98602.1.
BK006938 Genomic DNA. Translation: DAA11813.1.
PIRiS67576.
RefSeqiNP_010241.1. NM_001180102.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DGWX-ray3.11C149-266[»]
ProteinModelPortaliQ07350.
SMRiQ07350. Positions 149-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32016. 100 interactions.
DIPiDIP-1001N.
IntActiQ07350. 14 interactions.
MINTiMINT-383970.

PTM databases

iPTMnetiQ07350.

Proteomic databases

MaxQBiQ07350.
PeptideAtlasiQ07350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL043C; YDL043C; YDL043C.
GeneIDi851518.
KEGGisce:YDL043C.

Organism-specific databases

EuPathDBiFungiDB:YDL043C.
SGDiS000002201. PRP11.

Phylogenomic databases

GeneTreeiENSGT00720000108823.
HOGENOMiHOG000191688.
InParanoidiQ07350.
KOiK12826.
OMAiPCETIAI.
OrthoDBiEOG77HDS9.

Enzyme and pathway databases

BioCyciYEAST:G3O-29463-MONOMER.

Miscellaneous databases

NextBioi968893.
PROiQ07350.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs."
    Saren A.-M., Laamanen P., Lejarcegui J.B., Paulin L.
    Yeast 13:65-71(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif."
    Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr.
    Mol. Cell. Biol. 18:5000-5009(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CUS2.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP11_YEAST
AccessioniPrimary (citable) accession number: Q07350
Secondary accession number(s): D6VRV3, Q05434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3460 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.