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Q07346

- DCE_PETHY

UniProt

Q07346 - DCE_PETHY

Protein

Glutamate decarboxylase

Gene

GAD

Organism
Petunia hybrida (Petunia)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.

    GO - Molecular functioni

    1. glutamate decarboxylase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glutamate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Ligandi

    Calmodulin-binding, Pyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase (EC:4.1.1.15)
    Short name:
    GAD
    Gene namesi
    Name:GAD
    OrganismiPetunia hybrida (Petunia)
    Taxonomic identifieri4102 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Glutamate decarboxylasePRO_0000146976Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei277 – 2771N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiQ07346.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    500
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi474 – 49219

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NWDNMR-B/C470-495[»]
    ProteinModelPortaliQ07346.
    SMRiQ07346. Positions 469-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ07346.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni469 – 50032Calmodulin-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q07346-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLSKTVSQS DVSIHSTFAS RYVRTSLPRF KMPDNSIPKE AAYQIINDEL    50
    MLDGNPRLNL ASFVTTWMEP ECDKLMMDSI NKNYVDMDEY PVTTELQNRC 100
    VNMIAHLFNA PLEDGETAVG VGTVGSSEAI MLAGLAFKRK WQNKMKAQGK 150
    PCDKPNIVTG ANVQVCWEKF ARYFEVELKE VKLSEGYYVM DPEKAVEMVD 200
    ENTICVAAIL GSTLNGEFED VKRLNDLLVE KNKETGWDTP IHVDAASGGF 250
    IAPFIYPELE WDFRLPLVKS INVSGHKYGL VYAGIGWVVW RNKDDLPDEL 300
    IFHINYLGAD QPTFTLNFSK GSSQVIAQYY QLIRLGYEGY KNVMENCQEN 350
    ASVLREGLEK TGRFNIISKE IGVPLVAFSL KDNRQHNEFE ISETLRRFGW 400
    IVPAYTMPPN AQHITVLRVV IREDFSRTLA ERLVRDIEKV LHELDTLPAR 450
    VNAKLAVAEE QAAANGSEVH KKTDSEVQLE MITAWKKFVE EKKKKTNRVC 500
    Length:500
    Mass (Da):56,726
    Last modified:November 1, 1995 - v1
    Checksum:i72A043CB885AE10D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16797 mRNA. Translation: AAA33709.1.
    L16977 mRNA. Translation: AAA33710.1.
    PIRiA48767.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16797 mRNA. Translation: AAA33709.1 .
    L16977 mRNA. Translation: AAA33710.1 .
    PIRi A48767.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NWD NMR - B/C 470-495 [» ]
    ProteinModelPortali Q07346.
    SMRi Q07346. Positions 469-495.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q07346.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q07346.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    InterProi IPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01788. Glu-decarb-GAD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A plant glutamate decarboxylase containing a calmodulin binding domain. Cloning, sequence, and functional analysis."
      Baum G., Chen Y., Arazi T., Takatsuji H., Fromm H.
      J. Biol. Chem. 268:19610-19617(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Petal.
    2. "Structural basis for simultaneous binding of two carboxy-terminal peptides of plant glutamate decarboxylase to calmodulin."
      Yap K.L., Yuan T., Mal T.K., Vogel H.J., Ikura M.
      J. Mol. Biol. 328:193-204(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 470-495 IN COMPLEX WITH CALMODULIN, DIMERIZATION.

    Entry informationi

    Entry nameiDCE_PETHY
    AccessioniPrimary (citable) accession number: Q07346
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3