Q07343 (PDE4B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 4B
EC=3.1.4.17

Alternative name(s):
DPDE4
PDE32

Gene names

Name:	PDE4B
Synonyms:	DPDE4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. Ref.9 Ref.10
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.9 Ref.10
Enzyme regulation	Inhibited by rolipram.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Tissue specificity	Expressed in brain, heart, lung and skeletal muscle.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Sequence caution	The sequence AAA35643.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway cellular response to drug Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara neutrophil homeostasis Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cell periphery Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Traceable author statement Ref.3. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform PDE4B1 (identifier: Q07343-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PDE4B2 (identifier: Q07343-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: MKKSRSVMTV...QPPVSRVNPQ → MKEHGGTFSS...PNYMPVCLFA

Isoform PDE4B3 (identifier: Q07343-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: MKKSRSVMTV...SIAITTVSQE → MTAKDSSKEL...QRRRFTVAHT

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 736

736

cAMP-specific 3',5'-cyclic phosphodiesterase 4B

PRO_0000198809

Regions

Nucleotide binding

406 – 410

5

cAMP

Sites

Active site

406

1

Proton donor

Metal binding

410

1

Divalent metal cation 1

Metal binding

446

1

Divalent metal cation 1

Metal binding

447

1

Divalent metal cation 1

Metal binding

447

1

Divalent metal cation 2

Metal binding

564

1

Divalent metal cation 1

Binding site

447

1

cAMP

Binding site

564

1

cAMP

Binding site

615

1

cAMP

Site

567

1

Binds AMP, but not cAMP By similarity

Amino acid modifications

Modified residue

184

1

Phosphothreonine By similarity

Modified residue

272

1

Phosphoserine By similarity

Modified residue

274

1

Phosphotyrosine By similarity

Natural variations

Alternative sequence

1 – 211

211

MKKSR…RVNPQ → MKEHGGTFSSTGISGGSGDS AMDSLQPLQPNYMPVCLFA in isoform PDE4B2.

VSP_004572

Alternative sequence

1 – 93

93

MKKSR…TVSQE → MTAKDSSKELTASEPEVCIK TFKEQMHLELELPRLPGNRP TSPKISPRSSPRNSPCFFRK LLVNKSIRQRRRFTVAHT in isoform PDE4B3.

VSP_004571

Natural variant

703

1

S → C.
Corresponds to variant rs2227297 [ dbSNP | Ensembl ].

VAR_034373

Secondary structure

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736

Helix Strand Turn

Details...

Helix

248 – 264

17

Helix

266 – 278

13

Helix

324 – 327

4

Beta strand

328 – 330

3

Helix

332 – 342

11

Turn

343 – 346

4

Helix

352 – 358

7

Turn

359 – 361

3

Helix

363 – 374

12

Helix

377 – 380

4

Helix

385 – 397

13

Beta strand

403 – 407

5

Helix

408 – 422

15

Helix

425 – 427

3

Turn

428 – 430

3

Helix

433 – 445

13

Turn

446 – 449

4

Helix

455 – 460

6

Helix

464 – 468

5

Turn

469 – 471

3

Helix

474 – 485

12

Helix

486 – 488

3

Helix

490 – 492

3

Turn

494 – 497

4

Helix

500 – 515

16

Helix

519 – 521

3

Helix

522 – 534

13

Beta strand

540 – 542

3

Helix

549 – 564

16

Helix

567 – 569

3

Helix

572 – 595

24

Helix

602 – 604

3

Turn

606 – 608

3

Helix

611 – 621

11

Helix

623 – 634

12

Turn

635 – 638

4

Helix

639 – 654

16

Beta strand

656 – 658

3

Beta strand

659 – 662

4

Helix

664 – 666

3

Helix

671 – 677

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform PDE4B1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 208FCE9CD40EF5EB	FASTA	736	83,343
	10 20 30 40 50 60 MKKSRSVMTV MADDNVKDYF ECSLSKSYSS SSNTLGIDLW RGRRCCSGNL QLPPLSQRQS 70 80 90 100 110 120 ERARTPEGDG ISRPTTLPLT TLPSIAITTV SQECFDVENG PSPGRSPLDP QASSSAGLVL 130 140 150 160 170 180 HATFPGHSQR RESFLYRSDS DYDLSPKAMS RNSSLPSEQH GDDLIVTPFA QVLASLRSVR 190 200 210 220 230 240 NNFTILTNLH GTSNKRSPAA SQPPVSRVNP QEESYQKLAM ETLEELDWCL DQLETIQTYR 250 260 270 280 290 300 SVSEMASNKF KRMLNRELTH LSEMSRSGNQ VSEYISNTFL DKQNDVEIPS PTQKDREKKK 310 320 330 340 350 360 KQQLMTQISG VKKLMHSSSL NNTSISRFGV NTENEDHLAK ELEDLNKWGL NIFNVAGYSH 370 380 390 400 410 420 NRPLTCIMYA IFQERDLLKT FRISSDTFIT YMMTLEDHYH SDVAYHNSLH AADVAQSTHV 430 440 450 460 470 480 LLSTPALDAV FTDLEILAAI FAAAIHDVDH PGVSNQFLIN TNSELALMYN DESVLENHHL 490 500 510 520 530 540 AVGFKLLQEE HCDIFMNLTK KQRQTLRKMV IDMVLATDMS KHMSLLADLK TMVETKKVTS 550 560 570 580 590 600 SGVLLLDNYT DRIQVLRNMV HCADLSNPTK SLELYRQWTD RIMEEFFQQG DKERERGMEI 610 620 630 640 650 660 SPMCDKHTAS VEKSQVGFID YIVHPLWETW ADLVQPDAQD ILDTLEDNRN WYQSMIPQSP 670 680 690 700 710 720 SPPLDEQNRD CQGLMEKFQF ELTLDEEDSE GPEKEGEGHS YFSSTKTLCV IDPENRDSLG 730 ETDIDIATED KSPVDT
	Isoform PDE4B2 [UniParc]. Checksum: A4F9FA0C5DB43380	FASTA	564	64,352
	10 20 30 40 50 60 MKEHGGTFSS TGISGGSGDS AMDSLQPLQP NYMPVCLFAE ESYQKLAMET LEELDWCLDQ 70 80 90 100 110 120 LETIQTYRSV SEMASNKFKR MLNRELTHLS EMSRSGNQVS EYISNTFLDK QNDVEIPSPT 130 140 150 160 170 180 QKDREKKKKQ QLMTQISGVK KLMHSSSLNN TSISRFGVNT ENEDHLAKEL EDLNKWGLNI 190 200 210 220 230 240 FNVAGYSHNR PLTCIMYAIF QERDLLKTFR ISSDTFITYM MTLEDHYHSD VAYHNSLHAA 250 260 270 280 290 300 DVAQSTHVLL STPALDAVFT DLEILAAIFA AAIHDVDHPG VSNQFLINTN SELALMYNDE 310 320 330 340 350 360 SVLENHHLAV GFKLLQEEHC DIFMNLTKKQ RQTLRKMVID MVLATDMSKH MSLLADLKTM 370 380 390 400 410 420 VETKKVTSSG VLLLDNYTDR IQVLRNMVHC ADLSNPTKSL ELYRQWTDRI MEEFFQQGDK 430 440 450 460 470 480 ERERGMEISP MCDKHTASVE KSQVGFIDYI VHPLWETWAD LVQPDAQDIL DTLEDNRNWY 490 500 510 520 530 540 QSMIPQSPSP PLDEQNRDCQ GLMEKFQFEL TLDEEDSEGP EKEGEGHSYF SSTKTLCVID 550 560 PENRDSLGET DIDIATEDKS PVDT
	Isoform PDE4B3 [UniParc]. Checksum: 49618FFEAFF33D30	FASTA	721	82,096
	10 20 30 40 50 60 MTAKDSSKEL TASEPEVCIK TFKEQMHLEL ELPRLPGNRP TSPKISPRSS PRNSPCFFRK 70 80 90 100 110 120 LLVNKSIRQR RRFTVAHTCF DVENGPSPGR SPLDPQASSS AGLVLHATFP GHSQRRESFL 130 140 150 160 170 180 YRSDSDYDLS PKAMSRNSSL PSEQHGDDLI VTPFAQVLAS LRSVRNNFTI LTNLHGTSNK 190 200 210 220 230 240 RSPAASQPPV SRVNPQEESY QKLAMETLEE LDWCLDQLET IQTYRSVSEM ASNKFKRMLN 250 260 270 280 290 300 RELTHLSEMS RSGNQVSEYI SNTFLDKQND VEIPSPTQKD REKKKKQQLM TQISGVKKLM 310 320 330 340 350 360 HSSSLNNTSI SRFGVNTENE DHLAKELEDL NKWGLNIFNV AGYSHNRPLT CIMYAIFQER 370 380 390 400 410 420 DLLKTFRISS DTFITYMMTL EDHYHSDVAY HNSLHAADVA QSTHVLLSTP ALDAVFTDLE 430 440 450 460 470 480 ILAAIFAAAI HDVDHPGVSN QFLINTNSEL ALMYNDESVL ENHHLAVGFK LLQEEHCDIF 490 500 510 520 530 540 MNLTKKQRQT LRKMVIDMVL ATDMSKHMSL LADLKTMVET KKVTSSGVLL LDNYTDRIQV 550 560 570 580 590 600 LRNMVHCADL SNPTKSLELY RQWTDRIMEE FFQQGDKERE RGMEISPMCD KHTASVEKSQ 610 620 630 640 650 660 VGFIDYIVHP LWETWADLVQ PDAQDILDTL EDNRNWYQSM IPQSPSPPLD EQNRDCQGLM 670 680 690 700 710 720 EKFQFELTLD EEDSEGPEKE GEGHSYFSST KTLCVIDPEN RDSLGETDID IATEDKSPVD T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cDNA of a human lymphocyte cyclic-AMP phosphodiesterase (PDE IV) reveals a multigene family." Obernolte R., Bhakta S., Alvarez R., Bach C., Mulkins M., Jarnagin K., Shelton E.R. Gene 129:239-247(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4B2).
[2]	"A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs." Bolger G., Michaeli T., Martins T., St John T., Steiner B., Rodgers L., Riggs M., Wigler M., Ferguson K. Mol. Cell. Biol. 13:6558-6571(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PDE4B1 AND PDE4B2). Tissue: Brain.
[3]	"Molecular cloning and transient expression in COS7 cells of a novel human PDE4B cAMP-specific phosphodiesterase, HSPDE4B3." Huston E., Lumb S., Russell A., Catterall C., Ross A.H., Steele M.R., Bolger G.B., Perry M.J., Owens R.J., Houslay M.D. Biochem. J. 328:549-558(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4B3).
[4]	"A low-Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain. Cloning and expression of cDNA, biochemical characterization of recombinant protein, and tissue distribution of mRNA." McLaughlin M.M., Cieslinski L.B., Burman M., Torphy T.J., Livi G.P. J. Biol. Chem. 268:6470-6476(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4B2). Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS PDE4B1 AND PDE4B3). Tissue: Hippocampus.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PDE4B1). Tissue: Brain.
[9]	"Atomic structure of PDE4: insights into phosphodiesterase mechanism and specificity." Xu R.X., Hassell A.M., Vanderwall D., Lambert M.H., Holmes W.D., Luther M.A., Rocque W.J., Milburn M.V., Zhao Y., Ke H., Nolte R.T. Science 288:1822-1825(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 324-700 IN COMPLEX WITH METAL IONS, FUNCTION, COFACTOR.
[10]	"Crystal structures of the catalytic domain of phosphodiesterase 4B complexed with AMP, 8-Br-AMP, and rolipram." Xu R.X., Rocque W.J., Lambert M.H., Vanderwall D.E., Luther M.A., Nolte R.T. J. Mol. Biol. 337:355-365(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 324-700 IN COMPLEX WITH AMP; METAL IONS AND THE INHIBITOR ROLIPRAM, FUNCTION, COFACTOR.
[11]	"A glutamine switch mechanism for nucleotide selectivity by phosphodiesterases." Zhang K.Y.J., Card G.L., Suzuki Y., Artis D.R., Fong D., Gillette S., Hsieh D., Neiman J., West B.L., Zhang C., Milburn M.V., Kim S.-H., Schlessinger J., Bollag G. Mol. Cell 15:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 324-700 IN COMPLEX WITH AMP AND METAL IONS.
[12]	"Structural basis for the activity of drugs that inhibit phosphodiesterases." Card G.L., England B.P., Suzuki Y., Fong D., Powell B., Lee B., Luu C., Tabrizizad M., Gillette S., Ibrahim P.N., Artis D.R., Bollag G., Milburn M.V., Kim S.-H., Schlessinger J., Zhang K.Y.J. Structure 12:2233-2247(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 324-700 IN COMPLEX WITH METAL IONS AND INHIBITORS.
[13]	"A family of phosphodiesterase inhibitors discovered by cocrystallography and scaffold-based drug design." Card G.L., Blasdel L., England B.P., Zhang C., Suzuki Y., Gillette S., Fong D., Ibrahim P.N., Artis D.R., Bollag G., Milburn M.V., Kim S.-H., Schlessinger J., Zhang K.Y.J. Nat. Biotechnol. 23:201-207(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 324-700 IN COMPLEX WITH METAL IONS AND INHIBITORS.
[14]	"Structures of the four subfamilies of phosphodiesterase-4 provide insight into the selectivity of their inhibitors." Wang H., Peng M.-S., Chen Y., Geng J., Robinson H., Houslay M.D., Cai J., Ke H. Biochem. J. 408:193-201(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 324-659 IN COMPLEX WITH METAL IONS AND THE INHIBITOR NVP.
[15]	"Pyrazolopyridines as a novel structural class of potent and selective PDE4 inhibitors." Hamblin J.N., Angell T.D.R., Ballantine S.P., Cook C.M., Cooper A.W.J., Dawson J., Delves C.J., Jones P.S., Lindvall M., Lucas F.S., Mitchell C.J., Neu M.Y., Ranshaw L.E., Solanke Y.E., Somers D.O., Wiseman J.O. Bioorg. Med. Chem. Lett. 18:4237-4241(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 324-675 IN COMPLEX WITH METAL IONS AND INHIBITOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12686 mRNA. Translation: AAA35643.1. Different initiation.
L20966 mRNA. Translation: AAA03589.1.
L20971 mRNA. Translation: AAA03593.1.
U85048 mRNA. Translation: AAB96381.1.
M97515 mRNA. Translation: AAA36426.1.
AK289969 mRNA. Translation: BAF82658.1.
AK290006 mRNA. Translation: BAF82695.1.
AL592285 , AL109926, AL357273, AL359701 Genomic DNA. Translation: CAH70626.1.
AL592285 , AL109926, AL357273, AL359701 Genomic DNA. Translation: CAH70628.1.
AL357273 , AL109926, AL359701, AL592285 Genomic DNA. Translation: CAH73002.1.
AL357273 , AL109926, AL359701, AL592285 Genomic DNA. Translation: CAH73004.1.
AL109926 , AL357273, AL359701, AL592285 Genomic DNA. Translation: CAI21749.1.
AL109926, AL359701 Genomic DNA. Translation: CAI21750.1.
AL109926 , AL357273, AL359701, AL592285 Genomic DNA. Translation: CAI21751.1.
AL359701 , AL109926, AL357273, AL592285 Genomic DNA. Translation: CAI23099.1.
AL359701, AL109926 Genomic DNA. Translation: CAI23100.1.
AL359701 , AL109926, AL357273, AL592285 Genomic DNA. Translation: CAI23101.1.
AL513493 Genomic DNA. No translation available.
AL590783 Genomic DNA. No translation available.
AL591487 Genomic DNA. No translation available.
CH471059 Genomic DNA. Translation: EAX06524.1.
BC101480 mRNA. Translation: AAI01481.1.
BC105040 mRNA. Translation: AAI05041.1.

IPI

IPI00016604.
IPI00183599.
IPI00220621.
IPI00746760.

PIR

I61354.

RefSeq

NP_001032416.1. NM_001037339.1.
NP_001032417.1. NM_001037340.1.
NP_001032418.1. NM_001037341.1.
NP_002591.2. NM_002600.3.

UniGene

Hs.198072.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F0J	X-ray	1.77	A/B	324-700	[»]
1JP1	model	-	A	324-700	[»]
1JP2	model	-	A	324-700	[»]
1RO6	X-ray	2.00	A/B	324-700	[»]
1RO9	X-ray	2.13	A/B	324-700	[»]
1ROR	X-ray	2.00	A/B	324-700	[»]
1TB5	X-ray	2.15	A/B	324-700	[»]
1XLX	X-ray	2.19	A/B	324-700	[»]
1XLZ	X-ray	2.06	A/B	324-700	[»]
1XM4	X-ray	2.31	A/B	324-700	[»]
1XM6	X-ray	1.92	A/B	324-700	[»]
1XMU	X-ray	2.30	A/B	324-700	[»]
1XMY	X-ray	2.40	A/B	324-700	[»]
1XN0	X-ray	2.31	A/B	324-700	[»]
1XOS	X-ray	2.28	A	324-700	[»]
1XOT	X-ray	2.34	A/B	324-700	[»]
1Y2H	X-ray	2.40	A/B	324-700	[»]
1Y2J	X-ray	2.55	A/B	324-700	[»]
2CHM	X-ray	1.60	A	450-475	[»]
2QYL	X-ray	1.95	A	324-659	[»]
3D3P	X-ray	1.75	A	324-675	[»]
3FRG	X-ray	1.70	A	324-675	[»]
3G45	X-ray	2.63	A/B	241-659	[»]
3GWT	X-ray	1.75	A	324-675	[»]
3HC8	X-ray	1.79	A	451-474	[»]
3HDZ	X-ray	1.80	A	451-474	[»]
3HMV	X-ray	2.23	A/B	324-700	[»]
3KKT	X-ray	2.48	A/B	324-700	[»]
3LY2	X-ray	2.60	A/B/C/D/E/F/G/H	324-659	[»]
3O0J	X-ray	1.95	A	334-656	[»]
3O56	X-ray	2.42	A	324-675	[»]
3O57	X-ray	2.00	A	324-675	[»]

ProteinModelPortal

Q07343.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q07343. 3 interactions.

STRING

9606.ENSP00000332116.

PTM databases

PhosphoSite

Q07343.

Polymorphism databases

DMDM

729163.

Proteomic databases

PaxDb

Q07343.

PRIDE

Q07343.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000329654; ENSP00000332116; ENSG00000184588.
ENST00000341517; ENSP00000342637; ENSG00000184588.
ENST00000371045; ENSP00000360084; ENSG00000184588.
ENST00000371049; ENSP00000360088; ENSG00000184588.
ENST00000423207; ENSP00000392947; ENSG00000184588.

GeneID

5142.

KEGG

hsa:5142.

UCSC

uc001dcn.3. human.

Organism-specific databases

CTD

5142.

GeneCards

GC01P066339.

HGNC

HGNC:8781. PDE4B.

HPA

HPA003005.

MIM

600127. gene.

neXtProt

NX_Q07343.

PharmGKB

PA33129.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG122287.

HOGENOM

HOG000236297.

HOVERGEN

HBG108239.

InParanoid

Q07343.

KO

K01120.

OMA

GEGHNYF.

OrthoDB

EOG4ZGPBT.

PhylomeDB

Q07343.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

UniPathway

UPA00762; UER00747.

Gene expression databases

ArrayExpress

Q07343.

Bgee

Q07343.

CleanEx

HS_PDE4B.

Genevestigator

Q07343.

GermOnline

ENSG00000184588. Homo sapiens.

Family and domain databases

Gene3D

1.10.1300.10. 1 hit.

InterPro

IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]

Pfam

PF00233. PDEase_I. 1 hit.
[Graphical view]

PRINTS

PR00387. PDIESTERASE1.

SMART

SM00471. HDc. 1 hit.
[Graphical view]

PROSITE

PS00126. PDEASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q07343.

ChEMBL

CHEMBL275.

ChiTaRS

PDE4B. human.

DrugBank

DB00131. Adenosine monophosphate.
DB01427. Amrinone.
DB00201. Caffeine.
DB01166. Cilostazol.
DB00651. Dyphylline.
DB00824. Enprofylline.
DB01113. Papaverine.
DB00806. Pentoxifylline.
DB00277. Theophylline.

EvolutionaryTrace

Q07343.

GenomeRNAi

5142.

NextBio

19828.

SOURCE

Search...

Entry information

Entry name

PDE4B_HUMAN

Accession

Primary (citable) accession number: Q07343
Secondary accession number(s): O15443 , Q13945, Q5TEK4, Q5TEK5, Q5TEK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.