Q07326 (PIGF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol-glycan biosynthesis class F protein
Short name=PIG-F

Alternative name(s):
GPI11 homolog

Gene names

Name:

PIGF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Forms a complex with PIGG and PIGO. PIGF is required to stabilize PIGG and PIGO.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PIGF family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C-terminal protein lipidation Traceable author statement. Source: Reactome preassembly of GPI anchor in ER membrane Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ethanolaminephosphotransferase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q07326-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q07326-2)

The sequence of this isoform differs from the canonical sequence as follows:
     183-219: VWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN → MTVERKRSTYRSLHVPCRGLGTVK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Phosphatidylinositol-glycan biosynthesis class F protein		PRO_0000191759
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential
Natural variations
	Alternative sequence	183 – 219	37	VWPIS…TYKNN → MTVERKRSTYRSLHVPCRGL GTVK in isoform 2.		VSP_004361

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 313F97A55B781EBF	FASTA	219	24,890
	10 20 30 40 50 60 MKDNDIKRLL YTHLLCIFSI ILSVFIPSLF LENFSILETH LTWLCICSGF VTAVNLVLYL 70 80 90 100 110 120 VVKPNTSSKR SSLSHKVTGF LKCCIYFLMS CFSFHVIFVL YGAPLIELAL ETFLFAVILS 130 140 150 160 170 180 TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI WENSLQITTI SSFVGAWLGA LPIPLDWERP 190 200 210 WQVWPISCTL GATFGYVAGL VISPLWIYWN RKQLTYKNN
	Isoform 2 [UniParc]. Checksum: 2448DBF73784B1F4	FASTA	206	23,391
	10 20 30 40 50 60 MKDNDIKRLL YTHLLCIFSI ILSVFIPSLF LENFSILETH LTWLCICSGF VTAVNLVLYL 70 80 90 100 110 120 VVKPNTSSKR SSLSHKVTGF LKCCIYFLMS CFSFHVIFVL YGAPLIELAL ETFLFAVILS 130 140 150 160 170 180 TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI WENSLQITTI SSFVGAWLGA LPIPLDWERP 190 200 WQMTVERKRS TYRSLHVPCR GLGTVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human gene, PIG-F, a component of glycosylphosphatidylinositol anchor biosynthesis, by a novel expression cloning strategy." Inoue N., Kinoshita T., Orii T., Takeda J. J. Biol. Chem. 268:6882-6885(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[3]	"GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol." Shishioh N., Hong Y., Ohishi K., Ashida H., Maeda Y., Kinoshita T. J. Biol. Chem. 280:9728-9734(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIGG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13435 mRNA. Translation: BAA02697.1. BC021725 mRNA. Translation: AAH21725.1. BC029408 mRNA. Translation: AAH29408.1.
IPI	IPI00016536. IPI00220276.
PIR	A46097.
RefSeq	NP_002634.1. NM_002643.3. NP_775097.1. NM_173074.2.
UniGene	Hs.468415.
3D structure databases
ProteinModelPortal	Q07326.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000281382.
Polymorphism databases
DMDM	730326.
Proteomic databases
PRIDE	Q07326.
Protocols and materials databases
DNASU	5281.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000281382; ENSP00000281382; ENSG00000151665. ENST00000306465; ENSP00000302663; ENSG00000151665.
GeneID	5281.
KEGG	hsa:5281.
UCSC	uc002rvc.3. human. uc002rvd.3. human.
Organism-specific databases
CTD	5281.
GeneCards	GC02M046808.
HGNC	HGNC:8962. PIGF.
HPA	HPA051389.
MIM	600153. gene.
neXtProt	NX_Q07326.
PharmGKB	PA33293.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148715.
HOGENOM	HOG000082526.
HOVERGEN	HBG031820.
InParanoid	Q07326.
KO	K05287.
OMA	NGAMSIW.
OrthoDB	EOG4K0QPJ.
PhylomeDB	Q07326.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
UniPathway	UPA00196.
Gene expression databases
ArrayExpress	Q07326.
Bgee	Q07326.
CleanEx	HS_PIGF.
Genevestigator	Q07326.
GermOnline	ENSG00000151665. Homo sapiens.
Family and domain databases
InterPro	IPR009580. GPI_biosynthesis_protein_Pig-F. [Graphical view]
PANTHER	PTHR15095. PTHR15095. 1 hit.
Pfam	PF06699. PIG-F. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5281.
NextBio	20408.
SOURCE	Search...

Entry information

Entry name

PIGF_HUMAN

Accession

Primary (citable) accession number: Q07326
Secondary accession number(s): Q8WW20

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.