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Reviewed, UniProtKB/Swiss-Prot Q07310 (NRX3A_RAT)

Last modified June 16, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Neurexin-3-alpha
Alternative name(s):
    Neurexin III-alpha
Gene names
Name: Nrxn3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1578 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling.

Subunit structure

The laminin G-like domain 2 binds to NXPH1. Isoforms alpha 4B bind to alpha-dystroglycan. The cytoplasmic C-terminal region binds to CASK.

Subcellular location

Cell membrane; Single-pass type I membrane protein Potential. Secreted. Note: Some isoforms seems to be secreted.

Tissue specificity

Brain.

Sequence similarities

Belongs to the neurexin family.

Contains 3 EGF-like domains.

Contains 6 laminin G-like domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
Secreted
   Coding sequence diversityAlternative splicing
   DomainEGF-like domain
Repeat
Signal
Transmembrane
   LigandCalcium
Metal-binding
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 19 isoforms produced by alternative splicing. [Align] [Select]

Note: There are five major alternatively spliced sites, each of which may be spliced in up to twelve different ways. Combinatorial splicing at each of these five sites may lead to the generation of at least 288 isoforms but for simplicity only individual splice events are explicitly described below. Isoforms Alpha 5A to isoform Alpha 5H lack the transmembrane domain. Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q07310-1)

Also known as: Alpha-5I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q07310-2)

Also known as: Alpha-1B;

The sequence of this isoform differs from the canonical sequence as follows:
     253-256: Missing.
Isoform 3 (identifier: Q07310-3)

Also known as: Alpha-1C;

The sequence of this isoform differs from the canonical sequence as follows:
     237-242: Missing.
Isoform 4 (identifier: Q07310-4)

Also known as: Alpha-1D;

The sequence of this isoform differs from the canonical sequence as follows:
     237-242: Missing.
     253-256: Missing.
Isoform 5 (identifier: Q07310-5)

Also known as: Alpha-1E;

The sequence of this isoform differs from the canonical sequence as follows:
     243-256: Missing.
Isoform 6 (identifier: Q07310-6)

Also known as: Alpha-1F;

The sequence of this isoform differs from the canonical sequence as follows:
     237-256: Missing.
Isoform 7 (identifier: Q07310-7)

Also known as: Alpha-3B;

The sequence of this isoform differs from the canonical sequence as follows:
     754-763: DCIRINCNSS → G
Isoform 8 (identifier: Q07310-8)

Also known as: Alpha-4B;

The sequence of this isoform differs from the canonical sequence as follows:
     1210-1239: Missing.
Isoform 9 (identifier: Q07310-9)

Also known as: Alpha-5A;

The sequence of this isoform differs from the canonical sequence as follows:
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → ARSSNAARIT...LKFMCCHPPP
Isoform 10 (identifier: Q07310-10)

Also known as: Alpha-5B;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → ARSSNAARIT...LKFMCCHPPP
Isoform 11 (identifier: Q07310-11)

Also known as: Alpha-5C;

The sequence of this isoform differs from the canonical sequence as follows:
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → VLERRIILNLKTNAHPKSLQSKTC
Isoform 12 (identifier: Q07310-12)

Also known as: Alpha-5D;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → VLERRIILNLKTNAHPKSLQSKTC
Isoform 13 (identifier: Q07310-13)

Also known as: Alpha-5E;

The sequence of this isoform differs from the canonical sequence as follows:
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → DILLKSF
Isoform 14 (identifier: Q07310-14)

Also known as: Alpha-5F;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → DILLKSF
Isoform 15 (identifier: Q07310-15)

Also known as: Alpha-5G;

The sequence of this isoform differs from the canonical sequence as follows:
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → ATTTTKKSNFQECGNSICPRAFLHNFLL
Isoform 16 (identifier: Q07310-16)

Also known as: Alpha-5H;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
     1372-1478: Missing.
     1479-1578: ANPTEPGIRR...QKNKDKEYYV → ATTTTKKSNFQECGNSICPRAFLHNFLL
Isoform 17 (identifier: Q07310-17)

Also known as: Alpha-5J;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
Isoform 18 (identifier: Q07310-18)

Also known as: Alpha-5K;

The sequence of this isoform differs from the canonical sequence as follows:
     1372-1478: Missing.
Isoform 19 (identifier: Q07310-19)

Also known as: Alpha-5L;

The sequence of this isoform differs from the canonical sequence as follows:
     1369-1371: Missing.
     1372-1478: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727
Chain28 – 15781551Neurexin-3-alpha
PRO_0000019500

Regions

Topological domain28 – 15031476Extracellular Potential
Transmembrane1504 – 152421 Potential
Topological domain1525 – 157854Cytoplasmic Potential
Domain28 – 202175Laminin G-like 1
Domain198 – 23538EGF-like 1
Domain260 – 444185Laminin G-like 2
Domain451 – 643193Laminin G-like 3
Domain647 – 68438EGF-like 2
Domain689 – 861173Laminin G-like 4
Domain875 – 1050176Laminin G-like 5
Domain1053 – 109038EGF-like 3
Domain1094 – 1294201Laminin G-like 6
Compositional bias282 – 2854Poly-Ser
Compositional bias1323 – 13264Poly-Thr
Compositional bias1329 – 13324Poly-Thr
Compositional bias1407 – 14104Poly-Thr
Compositional bias1422 – 14254Poly-Ser
Compositional bias1511 – 15144Poly-Ala

Sites

Metal binding3081Calcium By similarity
Metal binding3251Calcium; via carbonyl oxygen By similarity
Metal binding3781Calcium; via carbonyl oxygen By similarity

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation1051N-linked (GlcNAc...) Potential
Glycosylation7611N-linked (GlcNAc...) Potential
Glycosylation11931N-linked (GlcNAc...) Potential
Glycosylation12911N-linked (GlcNAc...) Potential
Glycosylation13351N-linked (GlcNAc...) Potential
Glycosylation15001N-linked (GlcNAc...) Potential
Disulfide bond202 ↔ 213 By similarity
Disulfide bond207 ↔ 222 By similarity
Disulfide bond224 ↔ 234 By similarity
Disulfide bond408 ↔ 444 By similarity
Disulfide bond614 ↔ 643 By similarity
Disulfide bond651 ↔ 662 By similarity
Disulfide bond656 ↔ 671 By similarity
Disulfide bond673 ↔ 683 By similarity
Disulfide bond1022 ↔ 1050 By similarity
Disulfide bond1057 ↔ 1068 By similarity
Disulfide bond1062 ↔ 1077 By similarity
Disulfide bond1079 ↔ 1089 By similarity

Natural variations

Alternative sequence237 – 25620Missing in isoform 6.
VSP_003522
Alternative sequence237 – 2426Missing in isoform 3 and isoform 4.
VSP_003521
Alternative sequence243 – 25614Missing in isoform 5.
VSP_003523
Alternative sequence253 – 2564Missing in isoform 2 and isoform 4.
VSP_003524
Alternative sequence754 – 76310DCIRINCNSS → G in isoform 7.
VSP_003525
Alternative sequence1210 – 123930Missing in isoform 8.
VSP_003526
Alternative sequence1369 – 13713Missing in isoform 10, isoform 12, isoform 14, isoform 16, isoform 17 and isoform 19.
VSP_003527
Alternative sequence1372 – 1478107Missing in isoform 9, isoform 10, isoform 11, isoform 12, isoform 13, isoform 14, isoform 15, isoform 16, isoform 18 and isoform 19.
VSP_003528
Alternative sequence1479 – 1578100ANPTE…KEYYV → VLERRIILNLKTNAHPKSLQ SKTC in isoform 11 and isoform 12.
VSP_003530
Alternative sequence1479 – 1578100ANPTE…KEYYV → DILLKSF in isoform 13 and isoform 14.
VSP_003531
Alternative sequence1479 – 1578100ANPTE…KEYYV → ATTTTKKSNFQECGNSICPR AFLHNFLL in isoform 15 and isoform 16.
VSP_003532
Alternative sequence1479 – 1578100ANPTE…KEYYV → ARSSNAARITPCRPYMDMAT HLHIYPSHLHLLCSTLIDTP LPFPHPFFPMLPPSLALLKF MCCHPPP in isoform 9 and isoform 10.
VSP_003529

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha-5I) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 936CF8529143D0C7

FASTA1,578173,994
        10         20         30         40         50         60 
MSFTLHSVFF TLKVSSFLGS LVGLCLGLEF MGLPNQWARY LRWDASTRSD LSFQFKTNVS 

        70         80         90        100        110        120 
TGLLLYLDDG GVCDFLCLSL VDGRVQLRFS MDCAETTVLS NKQVNDSSWH FLMVSRDRVR 

       130        140        150        160        170        180 
TGLVIDGEGQ SGELRAQRPY MDVVSDLFLG GGPADIRPSA LTLDGVQNMP GFKGLMLDLK 

       190        200        210        220        230        240 
YGNSEPRLLG SQSVQLEAEG PCGERPCENG GICFLLDGHP TCDCSTTGYG GTLCSEDVSQ 

       250        260        270        280        290        300 
GPGLSHLMMS EQGRSKAREE NVATFRGSEY LSYDLSQNPI QSSSSEITLS FKTWQRNGLI 

       310        320        330        340        350        360 
LHTGKSADYV NLALKDGAVS LVINLGSGAF EAIVEPVNGK FNDNAWHDVK VTRNLRQVTI 

       370        380        390        400        410        420 
SVDGILTTTG YTQEDYTMLG SDDSSYVGPS PSTADLPGSP VSNNFMGCLK EVVYKNNDIR 

       430        440        450        460        470        480 
LELSRLARIG ATKMKIYGEV VFKCENVATL DPINFETPEA YISLPKWNTK RMGSISFDFR 

       490        500        510        520        530        540 
TTEPNGLILL THGKPQERKD VRSQKNTKVD FFAVELLDGN LYLLLDMGSG TIKVKATQKK 

       550        560        570        580        590        600 
ANDGEWYHVD IQRDGRSGTI SVNSRRTPFT ASGQSEILDL EGDMYLGGLP ENRAGLILPT 

       610        620        630        640        650        660 
ELWTAMLNYG YVGCIRDLFI DGRSKNIRQL AEMQNAAGVK SSCSRMSAKQ CDSYPCKNNA 

       670        680        690        700        710        720 
VCKDGWNRFI CDCTGTGYWG RTCEREASIL SYDGSMYMKV IMPMVMHTEA EDVSFRFMSQ 

       730        740        750        760        770        780 
RAYGLLVATT SRDSADTLRL ELDGGRVKLM VNLDCIRINC NSSKGPETLY AGQKLNDNEW 

       790        800        810        820        830        840 
HTVRVVRRGK SLKLTVDDDV AEGTMVGDHT RLEFHNIETG IMTEKRYISV VPSSFIGHLQ 

       850        860        870        880        890        900 
SLMFNGLLYI DLCKNGDIDY CELKARFGLR NIIADPVTFK TKSSYLTLAT LQAYTSMHLF 

       910        920        930        940        950        960 
FQFKTTSADG FILFNSGDGN NFIAVELVKG YIHYVFDLGN GPNVIKGNSD RPLNDNQWHN 

       970        980        990       1000       1010       1020 
VVITRDNSNT HSLKVDTKVV TQVINGAKNL DLKGDLYMAG LAQGMYSNLP KLVASRDGFQ 

      1030       1040       1050       1060       1070       1080 
GCLASVDLNG RLPDLINDAL HRSGQIDRGC EGPSTTCQED SCANQGVCMQ QWEGFTCDCS 

      1090       1100       1110       1120       1130       1140 
MTSYSGNQCN DPGATYIFGK SGGLILYTWP ANDRPSTRSD RLAVGFSTTV KDGVLVRIDS 

      1150       1160       1170       1180       1190       1200 
APGLGDFLQL HIEQGKIGVV FNIGTVDISI KEERTPVNDG KYHVVRFTRN GANATLQVDN 

      1210       1220       1230       1240       1250       1260 
WPVNEHYPTG NTDNERRQMV KQKIPFKYNR PVEEWLQEKG RQLTIFNTQA QIAIGGKDKG 

      1270       1280       1290       1300       1310       1320 
RLFQGQLSGL YYDGLKVLNM AAENNPNIKI NGSVRLVGEV PSVSGTTHTT SMPPEMSTTV 

      1330       1340       1350       1360       1370       1380 
METTTTMATT TTRKNRSTAS IQPTSDDLVS SAECSSDDED FVECEPSTGR SDKSLSTSIF 

      1390       1400       1410       1420       1430       1440 
EGGYKAHAPK WESKDFRPNK VSETSRTTTT SLSPELIRFT ASSSSGMVPK LPAGKMNNRD 

      1450       1460       1470       1480       1490       1500 
LKPQPDIVLL PLPTAYELDS TKLKSPLITC PMFRNVPTAN PTEPGIRRVP GASEVIRESN 

      1510       1520       1530       1540       1550       1560 
STTGMVVGIV AAAALCILIL LYAMYKYRNR DEGSYQVDET RNYISNSAQS NGTLMKEKQA 

      1570 
SSKSGHKKQK NKDKEYYV 

« Hide

Isoform 2 (Alpha-1B).

Checksum: A565F06AC79EBC8C
Show »

FASTA1,574173,565
Isoform 3 (Alpha-1C).

Checksum: 35F97FD2C2C71884
Show »

FASTA1,572173,410
Isoform 4 (Alpha-1D).

Checksum: 86E13829189881D9
Show »

FASTA1,568172,982
Isoform 5 (Alpha-1E).

Checksum: D34F1E83B6B42035
Show »

FASTA1,564172,451
Isoform 6 (Alpha-1F).

Checksum: A7AE5CCE2C75CE76
Show »

FASTA1,558171,867
Isoform 7 (Alpha-3B).

Checksum: B993492919356CC5
Show »

FASTA1,569172,945
Isoform 8 (Alpha-4B).

Checksum: 44A42D2AA6053D62
Show »

FASTA1,548170,250
Isoform 9 (Alpha-5A).

Checksum: 5DA4C69346C7FFF6
Show »

FASTA1,438158,689
Isoform 10 (Alpha-5B).

Checksum: 4DA00E05678DB01F
Show »

FASTA1,435158,388
Isoform 11 (Alpha-5C).

Checksum: F51D7F30DADE9E49
Show »

FASTA1,395153,889
Isoform 12 (Alpha-5D).

Checksum: F8D7669F3A237005
Show »

FASTA1,392153,589
Isoform 13 (Alpha-5E).

Checksum: FB88CCC4F7199BCB
Show »

FASTA1,378151,961
Isoform 14 (Alpha-5F).

Checksum: DA058AF65978461C
Show »

FASTA1,375151,660
Isoform 15 (Alpha-5G).

Checksum: EAEE734DFC9B091A
Show »

FASTA1,399154,268
Isoform 16 (Alpha-5H).

Checksum: 687D090AB15110B5
Show »

FASTA1,396153,968
Isoform 17 (Alpha-5J).

Checksum: BBAF52E01BDA3562
Show »

FASTA1,575173,693
Isoform 18 (Alpha-5K).

Checksum: CB2D693F873094A1
Show »

FASTA1,471162,216
Isoform 19 (Alpha-5L).

Checksum: 5E3B919BD0323E7F
Show »

FASTA1,468161,916

References

[1]"Neurexin III alpha: extensive alternative splicing generates membrane-bound and soluble forms."
Ushkaryov Y.A., Suedhof T.C.
Proc. Natl. Acad. Sci. U.S.A. 90:6410-6414(1993) [PubMed: 8341647] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cartography of neurexins: more than 1000 isoforms generated by alternative splicing and expressed in distinct subsets of neurons."
Ullrich B., Ushkaryov Y.A., Suedhof T.C.
Neuron 14:497-507(1995) [PubMed: 7695896] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Brain.
[3]"Neurexophilin binding to alpha-neurexins. A single LNS domain functions as an independently folding ligand-binding unit."
Missler M., Hammer R.E., Suedhof T.C.
J. Biol. Chem. 273:34716-34723(1998) [PubMed: 9856994] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, INTERACTION WITH NXPH1.
[4]"CASK: a novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins."
Hata Y., Butz S., Suedhof T.C.
J. Neurosci. 16:2488-2494(1996) [PubMed: 8786425] [Abstract]
Cited for: INTERACTION WITH CASK.
[5]"A stoichiometric complex of neurexins and dystroglycan in brain."
Sugita S., Saito F., Tang J., Satz J., Campbell K., Suedhof T.C.
J. Cell Biol. 154:435-445(2001) [PubMed: 11470830] [Abstract]
Cited for: INTERACTION WITH ALPHA-DYSTROGLYCAN.
+Additional computationally mapped references.

Cross-references

Sequence databases

L14851 mRNA. Translation: AAA02853.1.
L14851 mRNA. Translation: AAA02854.1.
L14851 mRNA. Translation: AAA02855.1.
L14851 mRNA. Translation: AAA02856.1.
L14851 mRNA. Translation: AAA02857.1.
L14851 mRNA. Translation: AAA02858.1.
IPIIPI00476688.
IPI00829422.
IPI00829428.
IPI00829441.
IPI00829447.
IPI00829448.
IPI00829450.
IPI00829453.
IPI00829456.
IPI00829461.
IPI00829465.
IPI00829472.
IPI00829491.
IPI00829492.
IPI00829495.
IPI00829499.
IPI00829500.
IPI00829503.
IPI00829509.
PIRI48216.
RefSeqNP_446269.2.
UniGeneRn.10926

3D structure databases

HSSPHSSP built from PDB template 1C4R based on UniProtKB Q63373.
SMRQ07310. Positions 258-438, 1093-1301.
ModBaseSearch...

Proteomic databases

PRIDEQ07310.

Genome annotation databases

EnsemblENSRNOG00000024722. Rattus norvegicus. [Contig view]
GeneID116508.
KEGGrno:116508.

Organism-specific databases

RGD620212. Nrxn3.

Phylogenomic databases

HOVERGENQ07310.

Gene expression databases

ArrayExpressQ07310.
GermOnlineENSRNOG00000024722. Rattus norvegicus.

Family and domain databases

InterProIPR013320. ConA-like_subgrp.
IPR006209. EGF.
IPR006210. EGF-like.
IPR013032. EGF-like_reg_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_CS.
IPR000742. EGF_3.
IPR001791. Laminin_G.
IPR012680. Laminin_G_2.
IPR003585. Neurexin-like.
IPR015447. NRX.
[Graphical view]
Gene3DG3DSA:2.60.120.200. ConA_like_subgrp. 6 hits.
PANTHERPTHR10127:SF88. NRX. 1 hit.
PfamPF00008. EGF. 3 hits.
PF02210. Laminin_G_2. 6 hits.
[Graphical view]
SMARTSM00294. 4.1m. 1 hit.
SM00181. EGF. 3 hits.
SM00282. LamG. 6 hits.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 3 hits.
PS50025. LAM_G_DOMAIN. 6 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio619125.

Entry information

Entry nameNRX3A_RAT
AccessionPrimary (citable) accession number: Q07310
Secondary accession number(s): Q07280 expand/collapse secondary AC list , Q07311, Q07312, Q07313, Q07314
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents