Reviewed, UniProtKB/Swiss-Prot Q07276 (KLK1_MACFA)

Names and origin

Protein names

Recommended name:
    Kallikrein-1
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    Kidney/pancreas/salivary gland kallikrein

Gene names

Name:

KLK1

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027925
	Chain	25 – 257	233	Kallikrein-1		PRO_0000027926
Regions
	Domain	25 – 254	230	Peptidase S1
Sites
	Active site	62	1	Charge relay system By similarity
	Active site	116	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	90	1	O-linked (GalNAc...) By similarity
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	O-linked (GalNAc...) By similarity
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	O-linked (GalNAc...) By similarity
	Disulfide bond	31 ↔ 169		By similarity
	Disulfide bond	47 ↔ 63		By similarity
	Disulfide bond	148 ↔ 215		By similarity
	Disulfide bond	180 ↔ 194		By similarity
	Disulfide bond	205 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07276-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 32774D4C069316A7	FASTA	257	28,237
	10 20 30 40 50 60 MWFLVLCLAL SLGGTGRAPP IQSRIVGGWE CSQPWQAALY HFSTFQCGGI LVHPQWVLTA 70 80 90 100 110 120 AHCISDNYQL WLGRHNLFDD EDTAQFVHVS ESFPHPGFNM SLLKNHTRQA DDYSHDLMLL 130 140 150 160 170 180 RLTQPAEITD AVQVVELPTQ EPEVGSTCLA SGWGSIEPEN FSFPDDLQCV DLEILPNDEC 190 200 210 220 230 240 AKAHTQKVTE FMLCAGHLEG GKDTCVGDSG GPLTCDGVLQ GVTSWGYIPC GSPNKPAVFV 250 KVLSYVKWIE DTIAENS

References

[1]

"Molecular cloning and sequence analysis of the monkey and human tissue kallikrein genes." Lin F.K., Lin C.H., Chou C., Chen K., Lu H.S., Bacheller B., Herrera C., Jones T., Chao J., Chao L. Biochim. Biophys. Acta 1173:325-328(1993) [PubMed: 7916636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10039 mRNA. Translation: AAA36853.1.
PIR	S33772.
3D structure databases
HSSP	HSSP built from PDB template 1FDP based on UniProtKB P00746.
SMR	Q07276. Positions 25-257.
ModBase	Search...
Protein family/group databases
MEROPS	S01.160.
Phylogenomic databases
HOVERGEN	Q07276.
Enzyme and pathway databases
BRENDA	3.4.21.35. 3438.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK1_MACFA

Accession

Primary (citable) accession number: Q07276

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	March 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)