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Q07272 (VP7_ROTH7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid glycoprotein VP7
OrganismRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A) [Complete proteome]
Taxonomic identifier578827 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.

Subunit structure

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.

Subcellular location

Virion By similarity. Host endoplasmic reticulum lumen Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Post-translational modification

The N-terminus is blocked possibly by pyroglutamic acid By similarity.

N-glycosylated By similarity.

Intramolecular disulfide bonds By similarity.

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Sequence similarities

Belongs to the rotavirus VP7 family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q07272-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q07272-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5050 Potential
Chain51 – 326276Outer capsid glycoprotein VP7 Potential
PRO_0000369101

Amino acid modifications

Glycosylation691N-linked (GlcNAc...); by host Potential
Glycosylation1451N-linked (GlcNAc...); by host Potential

Natural variations

Alternative sequence1 – 2929Missing in isoform 2.
VSP_038599

Experimental info

Sequence conflict481D → V in ABV53240. Ref.2
Sequence conflict243 – 2442SR → TC in ABV53240. Ref.2
Sequence conflict3091T → A in ABV53240. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6E9ACD4EEAFA526C

FASTA32637,506
        10         20         30         40         50         60 
MYGIEYTTFL IYLISIILFN YILKSITRMM DYIIYKFLLI ITITSIFDSA QNYGINLPIT 

        70         80         90        100        110        120 
GSMDVSYVNA TKDEPFLTST LCLYYPTEAR TEINDNEWTS TLSQLFLTKG WPTGSVYFKE 

       130        140        150        160        170        180 
YDDIPTFSVD PQLYCDYNIV LMRYNSSLKL DMSELANLIL NEWLCNPMDI TLYYYQQTDE 

       190        200        210        220        230        240 
ANKWIAMGQS CTIKVCPLNT QTLGIGCQTT NTGTFEEVAT AEKLVITDVV DGVNHKLDVT 

       250        260        270        280        290        300 
TASRTIRNCK KLGPRENVAV IQIGGADILD ITSDPTTTPQ TERMMRINWK KWWQVFYTIV 

       310        320 
DYVNQIVQTM SKRSRSLDSA AFYYRV 

« Hide

Isoform 2 [UniParc].

Checksum: A2B12E1223C8C556
Show »

FASTA29733,990

References

[1]"A serotype 10 human rotavirus."
Beards G., Xu L.I., Ballard A., Desselberger U., McCrae M.A.
J. Clin. Microbiol. 30:1432-1435(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63156 Genomic RNA. Translation: CAA44857.1.
EF672567 Genomic RNA. Translation: ABV53240.1.
PIRS48128.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001963. VP7.
[Graphical view]
PfamPF00434. VP7. 1 hit.
[Graphical view]
ProDomPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP7_ROTH7
AccessionPrimary (citable) accession number: Q07272
Secondary accession number(s): B3SRR5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families