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Q07272

- VP7_ROTH7

UniProt

Q07272 - VP7_ROTH7

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Protein
Outer capsid glycoprotein VP7
Gene
N/A
Organism
Rotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri578827 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001456: Genome

Subcellular locationi

Virion By similarity. Host endoplasmic reticulum lumen Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

GO - Cellular componenti

  1. T=13 icosahedral viral capsid Source: UniProtKB-KW
  2. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050 Reviewed prediction
Add
BLAST
Chaini51 – 326276Outer capsid glycoprotein VP7 Reviewed prediction
PRO_0000369101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid By similarity.
N-glycosylated By similarity.
Intramolecular disulfide bonds By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: Q07272-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYGIEYTTFL IYLISIILFN YILKSITRMM DYIIYKFLLI ITITSIFDSA    50
QNYGINLPIT GSMDVSYVNA TKDEPFLTST LCLYYPTEAR TEINDNEWTS 100
TLSQLFLTKG WPTGSVYFKE YDDIPTFSVD PQLYCDYNIV LMRYNSSLKL 150
DMSELANLIL NEWLCNPMDI TLYYYQQTDE ANKWIAMGQS CTIKVCPLNT 200
QTLGIGCQTT NTGTFEEVAT AEKLVITDVV DGVNHKLDVT TASRTIRNCK 250
KLGPRENVAV IQIGGADILD ITSDPTTTPQ TERMMRINWK KWWQVFYTIV 300
DYVNQIVQTM SKRSRSLDSA AFYYRV 326
Length:326
Mass (Da):37,506
Last modified:November 1, 1996 - v1
Checksum:i6E9ACD4EEAFA526C
GO
Isoform 2 (identifier: Q07272-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Show »
Length:297
Mass (Da):33,990
Checksum:iA2B12E1223C8C556
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2.
VSP_038599Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481D → V in ABV53240. 1 Publication
Sequence conflicti243 – 2442SR → TC in ABV53240. 1 Publication
Sequence conflicti309 – 3091T → A in ABV53240. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63156 Genomic RNA. Translation: CAA44857.1.
EF672567 Genomic RNA. Translation: ABV53240.1.
PIRiS48128.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63156 Genomic RNA. Translation: CAA44857.1 .
EF672567 Genomic RNA. Translation: ABV53240.1 .
PIRi S48128.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001963. VP7.
[Graphical view ]
Pfami PF00434. VP7. 1 hit.
[Graphical view ]
ProDomi PD000191. VP7. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "A serotype 10 human rotavirus."
    Beards G., Xu L.I., Ballard A., Desselberger U., McCrae M.A.
    J. Clin. Microbiol. 30:1432-1435(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP7_ROTH7
AccessioniPrimary (citable) accession number: Q07272
Secondary accession number(s): B3SRR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi