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Protein

Drebrin

Gene

Dbn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS (By similarity). Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites.By similarity

GO - Molecular functioni

  • actin filament binding Source: RGD
  • cytoskeletal protein binding Source: RGD
  • profilin binding Source: UniProtKB

GO - Biological processi

  • actin filament organization Source: UniProtKB
  • aging Source: RGD
  • brain development Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • cellular response to X-ray Source: RGD
  • lung alveolus development Source: RGD
  • modulation of inhibitory postsynaptic potential Source: RGD
  • negative regulation of actin-dependent ATPase activity Source: RGD
  • negative regulation of actin filament bundle assembly Source: RGD
  • negative regulation of locomotion involved in locomotory behavior Source: RGD
  • negative regulation of muscle filament sliding Source: RGD
  • nervous system development Source: RGD
  • positive regulation of axon extension Source: RGD
  • positive regulation of cell-substrate adhesion Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of excitatory postsynaptic potential Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of substrate adhesion-dependent cell spreading Source: RGD
  • prepulse inhibition Source: RGD
  • regulation of actin filament polymerization Source: RGD
  • regulation of filopodium assembly Source: RGD
  • regulation of postsynaptic density protein 95 clustering Source: RGD
  • response to activity Source: RGD
  • response to drug Source: RGD
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Drebrin
Alternative name(s):
Developmentally-regulated brain protein
Gene namesi
Name:Dbn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi70885. Dbn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcell cortex By similarity
  • Cell junction By similarity
  • Cell projection By similarity
  • Cell projectiongrowth cone By similarity

  • Note: In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC). Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • asymmetric synapse Source: RGD
  • axonal growth cone Source: RGD
  • axonal spine Source: RGD
  • cell-cell junction Source: RGD
  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • dendritic filopodium Source: RGD
  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • excitatory synapse Source: RGD
  • filopodium Source: RGD
  • growth cone Source: UniProtKB
  • lamellipodium Source: RGD
  • neuronal cell body Source: RGD
  • neuron spine Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: RGD
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000800102 – 707DrebrinAdd BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei141PhosphoserineBy similarity1
Modified residuei142PhosphoserineCombined sources1
Modified residuei241PhosphoserineBy similarity1
Modified residuei342PhosphoserineCombined sources1
Modified residuei377PhosphothreonineBy similarity1
Modified residuei381PhosphothreonineBy similarity1
Modified residuei383PhosphoserineBy similarity1
Modified residuei385PhosphoserineCombined sources1
Modified residuei391PhosphoserineBy similarity1
Modified residuei392PhosphothreonineBy similarity1
Modified residuei467PhosphoserineBy similarity1
Modified residuei549PhosphothreonineBy similarity1
Modified residuei659PhosphoserineBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07266.
PRIDEiQ07266.

PTM databases

iPTMnetiQ07266.
PhosphoSitePlusiQ07266.

Expressioni

Tissue specificityi

Brain neurons.

Gene expression databases

BgeeiENSRNOG00000014170.
ExpressionAtlasiQ07266. baseline and differential.
GenevisibleiQ07266. RN.

Interactioni

Subunit structurei

Interacts with RUFY3. Binds F-actin. Interacts with CXCR4; this interaction is enhanced by antigenic stimulation.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CXCR4P610733EBI-8786792,EBI-489411From a different organism.
PtenO548573EBI-918187,EBI-8074312
Zmynd8A8C4G99EBI-918187,EBI-9004258

GO - Molecular functioni

  • actin filament binding Source: RGD
  • cytoskeletal protein binding Source: RGD
  • profilin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi249553. 1 interactor.
IntActiQ07266. 4 interactors.
STRINGi10116.ENSRNOP00000019569.

Structurei

3D structure databases

ProteinModelPortaliQ07266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 134ADF-HPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Contains 1 ADF-H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IP00. Eukaryota.
ENOG410YAV7. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000015304.
HOVERGENiHBG000823.
InParanoidiQ07266.
OrthoDBiEOG091G05KP.
PhylomeDBiQ07266.
TreeFamiTF318935.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028438. Drebrin.
[Graphical view]
PANTHERiPTHR10829:SF1. PTHR10829:SF1. 2 hits.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q07266-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG
60 70 80 90 100
LQELSGHFEN QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS
110 120 130 140 150
HVAKVAEFFQ GVDVIVNASS VEDIDAGAIG QRLSNGLARL SSPVLHRLRL
160 170 180 190 200
REDENAEPVG TTYQKTDAAV EMKRINREQF WEQAKKEEEL RKEEERKKAL
210 220 230 240 250
DARLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ SLEAEEAKRR
260 270 280 290 300
LKDQSIFGDQ RDEEEESQMK KSESEVEEAA AIIAQRPDNP REFFRQQERV
310 320 330 340 350
ASASGGSCDA PSPFNHRPGR PYCPFIKASD SGPSSSSSSS SSPPRTPFPY
360 370 380 390 400
ITCHRTPNLS SSLPCSHLDS HRRMAPTPIP TRSPSDSSTA STPITEQIER
410 420 430 440 450
ALDEVTSSQP PPPPPPPPPA QEAQESAPRL DGEEVCKEAK VAAAPQVWAG
460 470 480 490 500
CAEEPPRAQE PPLLQSSPTE DLMCTESPEQ AVLAASPEPD ASVTSVADAH
510 520 530 540 550
AADTIETTTA TTATTIADNV TPAAASLIDL WPGNGEEAST PQAEPRVPTP
560 570 580 590 600
PSGAEASLAE VPLLNEAAQE PLPPVGEGCA NLLNFDELPE PPATFCDPEE
610 620 630 640 650
EAEGEPLAAS QVLTMPSALE EVDQVLEQEL EPEPHLLTNG ETTQKEGTQQ
660 670 680 690 700
ASEGYFSQSQ EEEFAQSEEP CAKAPPPVFY NKPPEIDITC WDADPVPEEE

EGFEGGD
Length:707
Mass (Da):77,472
Last modified:January 23, 2007 - v3
Checksum:iB5279BF6EB7B80AA
GO
Isoform E1 (identifier: Q07266-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-365: Missing.

Show »
Length:661
Mass (Da):72,602
Checksum:i0DE7B54E44D2ADFB
GO
Isoform E2 (identifier: Q07266-3)
Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti387S → P in BAA28746 (Ref. 2) Curated1
Sequence conflicti588L → R in BAA28746 (Ref. 2) Curated1
Sequence conflicti618A → V in BAA28746 (Ref. 2) Curated1
Sequence conflicti650Missing in AAI39848 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004201320 – 365Missing in isoform E1. 2 PublicationsAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59267 mRNA. Translation: CAA41957.1.
AB015042 mRNA. Translation: BAA28746.1.
BC139847 mRNA. Translation: AAI39848.1.
PIRiS60588.
RefSeqiNP_112286.1. NM_031024.1. [Q07266-1]
XP_006253719.1. XM_006253657.3.
UniGeneiRn.11247.

Genome annotation databases

EnsembliENSRNOT00000019393; ENSRNOP00000019393; ENSRNOG00000014170. [Q07266-2]
GeneIDi81653.
KEGGirno:81653.
UCSCiRGD:70885. rat. [Q07266-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59267 mRNA. Translation: CAA41957.1.
AB015042 mRNA. Translation: BAA28746.1.
BC139847 mRNA. Translation: AAI39848.1.
PIRiS60588.
RefSeqiNP_112286.1. NM_031024.1. [Q07266-1]
XP_006253719.1. XM_006253657.3.
UniGeneiRn.11247.

3D structure databases

ProteinModelPortaliQ07266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249553. 1 interactor.
IntActiQ07266. 4 interactors.
STRINGi10116.ENSRNOP00000019569.

PTM databases

iPTMnetiQ07266.
PhosphoSitePlusiQ07266.

Proteomic databases

PaxDbiQ07266.
PRIDEiQ07266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019393; ENSRNOP00000019393; ENSRNOG00000014170. [Q07266-2]
GeneIDi81653.
KEGGirno:81653.
UCSCiRGD:70885. rat. [Q07266-1]

Organism-specific databases

CTDi1627.
RGDi70885. Dbn1.

Phylogenomic databases

eggNOGiENOG410IP00. Eukaryota.
ENOG410YAV7. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000015304.
HOVERGENiHBG000823.
InParanoidiQ07266.
OrthoDBiEOG091G05KP.
PhylomeDBiQ07266.
TreeFamiTF318935.

Miscellaneous databases

PROiQ07266.

Gene expression databases

BgeeiENSRNOG00000014170.
ExpressionAtlasiQ07266. baseline and differential.
GenevisibleiQ07266. RN.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028438. Drebrin.
[Graphical view]
PANTHERiPTHR10829:SF1. PTHR10829:SF1. 2 hits.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDREB_RAT
AccessioniPrimary (citable) accession number: Q07266
Secondary accession number(s): A5D6R1, O70205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.