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Protein

Drebrin

Gene

Dbn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS (By similarity). Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites.By similarity

GO - Molecular functioni

  • actin filament binding Source: RGD
  • profilin binding Source: UniProtKB

GO - Biological processi

  • actin filament organization Source: UniProtKB
  • aging Source: RGD
  • brain development Source: RGD
  • cellular response to X-ray Source: RGD
  • lung alveolus development Source: RGD
  • modulation of inhibitory postsynaptic potential Source: RGD
  • negative regulation of actin-dependent ATPase activity Source: RGD
  • negative regulation of actin filament bundle assembly Source: RGD
  • negative regulation of locomotion involved in locomotory behavior Source: RGD
  • negative regulation of muscle filament sliding Source: RGD
  • nervous system development Source: RGD
  • positive regulation of axon extension Source: RGD
  • positive regulation of cell-substrate adhesion Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of excitatory postsynaptic potential Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of substrate adhesion-dependent cell spreading Source: RGD
  • prepulse inhibition Source: RGD
  • regulation of actin filament polymerization Source: RGD
  • regulation of filopodium assembly Source: RGD
  • regulation of postsynaptic density protein 95 clustering Source: RGD
  • response to activity Source: RGD
  • response to fluoxetine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Drebrin
Alternative name(s):
Developmentally-regulated brain protein
Gene namesi
Name:Dbn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi70885. Dbn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcell cortex By similarity
  • Cell junction By similarity
  • Cell projection By similarity
  • Cell projectiongrowth cone By similarity

  • Note: In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC). Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • asymmetric synapse Source: RGD
  • axonal growth cone Source: RGD
  • axonal spine Source: RGD
  • cell cortex Source: UniProtKB-SubCell
  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • dendritic filopodium Source: RGD
  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • excitatory synapse Source: RGD
  • filopodium Source: RGD
  • growth cone Source: UniProtKB
  • lamellipodium Source: RGD
  • neuronal cell body Source: RGD
  • neuron spine Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: RGD
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 707706DrebrinPRO_0000080010Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei141 – 1411PhosphoserineBy similarity
Modified residuei142 – 1421PhosphoserineCombined sources
Modified residuei241 – 2411PhosphoserineBy similarity
Modified residuei342 – 3421PhosphoserineCombined sources
Modified residuei377 – 3771PhosphothreonineBy similarity
Modified residuei381 – 3811PhosphothreonineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity
Modified residuei385 – 3851PhosphoserineCombined sources
Modified residuei391 – 3911PhosphoserineBy similarity
Modified residuei392 – 3921PhosphothreonineBy similarity
Modified residuei467 – 4671PhosphoserineBy similarity
Modified residuei549 – 5491PhosphothreonineBy similarity
Modified residuei659 – 6591PhosphoserineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07266.
PRIDEiQ07266.

PTM databases

iPTMnetiQ07266.
PhosphoSiteiQ07266.

Expressioni

Tissue specificityi

Brain neurons.

Gene expression databases

ExpressionAtlasiQ07266. baseline and differential.
GenevisibleiQ07266. RN.

Interactioni

Subunit structurei

Interacts with RUFY3. Binds F-actin. Interacts with CXCR4; this interaction is enhanced by antigenic stimulation.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CXCR4P610733EBI-8786792,EBI-489411From a different organism.
PtenO548573EBI-918187,EBI-8074312
Zmynd8A8C4G99EBI-918187,EBI-9004258

GO - Molecular functioni

  • actin filament binding Source: RGD
  • profilin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi249553. 1 interaction.
IntActiQ07266. 4 interactions.
STRINGi10116.ENSRNOP00000019569.

Structurei

3D structure databases

ProteinModelPortaliQ07266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 134132ADF-HPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ADF-H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IP00. Eukaryota.
ENOG410YAV7. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000015304.
HOVERGENiHBG000823.
InParanoidiQ07266.
OMAiCSHLDSH.
OrthoDBiEOG7X3QR9.
PhylomeDBiQ07266.
TreeFamiTF318935.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028438. Drebrin.
[Graphical view]
PANTHERiPTHR10829:SF1. PTHR10829:SF1. 2 hits.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q07266-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG
60 70 80 90 100
LQELSGHFEN QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS
110 120 130 140 150
HVAKVAEFFQ GVDVIVNASS VEDIDAGAIG QRLSNGLARL SSPVLHRLRL
160 170 180 190 200
REDENAEPVG TTYQKTDAAV EMKRINREQF WEQAKKEEEL RKEEERKKAL
210 220 230 240 250
DARLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ SLEAEEAKRR
260 270 280 290 300
LKDQSIFGDQ RDEEEESQMK KSESEVEEAA AIIAQRPDNP REFFRQQERV
310 320 330 340 350
ASASGGSCDA PSPFNHRPGR PYCPFIKASD SGPSSSSSSS SSPPRTPFPY
360 370 380 390 400
ITCHRTPNLS SSLPCSHLDS HRRMAPTPIP TRSPSDSSTA STPITEQIER
410 420 430 440 450
ALDEVTSSQP PPPPPPPPPA QEAQESAPRL DGEEVCKEAK VAAAPQVWAG
460 470 480 490 500
CAEEPPRAQE PPLLQSSPTE DLMCTESPEQ AVLAASPEPD ASVTSVADAH
510 520 530 540 550
AADTIETTTA TTATTIADNV TPAAASLIDL WPGNGEEAST PQAEPRVPTP
560 570 580 590 600
PSGAEASLAE VPLLNEAAQE PLPPVGEGCA NLLNFDELPE PPATFCDPEE
610 620 630 640 650
EAEGEPLAAS QVLTMPSALE EVDQVLEQEL EPEPHLLTNG ETTQKEGTQQ
660 670 680 690 700
ASEGYFSQSQ EEEFAQSEEP CAKAPPPVFY NKPPEIDITC WDADPVPEEE

EGFEGGD
Length:707
Mass (Da):77,472
Last modified:January 23, 2007 - v3
Checksum:iB5279BF6EB7B80AA
GO
Isoform E1 (identifier: Q07266-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-365: Missing.

Show »
Length:661
Mass (Da):72,602
Checksum:i0DE7B54E44D2ADFB
GO
Isoform E2 (identifier: Q07266-3)

Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti387 – 3871S → P in BAA28746 (Ref. 2) Curated
Sequence conflicti588 – 5881L → R in BAA28746 (Ref. 2) Curated
Sequence conflicti618 – 6181A → V in BAA28746 (Ref. 2) Curated
Sequence conflicti650 – 6501Missing in AAI39848 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei320 – 36546Missing in isoform E1. 2 PublicationsVSP_004201Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59267 mRNA. Translation: CAA41957.1.
AB015042 mRNA. Translation: BAA28746.1.
BC139847 mRNA. Translation: AAI39848.1.
PIRiS60588.
RefSeqiNP_112286.1. NM_031024.1. [Q07266-1]
XP_006253719.1. XM_006253657.2.
UniGeneiRn.11247.

Genome annotation databases

EnsembliENSRNOT00000019393; ENSRNOP00000019393; ENSRNOG00000014170. [Q07266-2]
GeneIDi81653.
KEGGirno:81653.
UCSCiRGD:70885. rat. [Q07266-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59267 mRNA. Translation: CAA41957.1.
AB015042 mRNA. Translation: BAA28746.1.
BC139847 mRNA. Translation: AAI39848.1.
PIRiS60588.
RefSeqiNP_112286.1. NM_031024.1. [Q07266-1]
XP_006253719.1. XM_006253657.2.
UniGeneiRn.11247.

3D structure databases

ProteinModelPortaliQ07266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249553. 1 interaction.
IntActiQ07266. 4 interactions.
STRINGi10116.ENSRNOP00000019569.

PTM databases

iPTMnetiQ07266.
PhosphoSiteiQ07266.

Proteomic databases

PaxDbiQ07266.
PRIDEiQ07266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019393; ENSRNOP00000019393; ENSRNOG00000014170. [Q07266-2]
GeneIDi81653.
KEGGirno:81653.
UCSCiRGD:70885. rat. [Q07266-1]

Organism-specific databases

CTDi1627.
RGDi70885. Dbn1.

Phylogenomic databases

eggNOGiENOG410IP00. Eukaryota.
ENOG410YAV7. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000015304.
HOVERGENiHBG000823.
InParanoidiQ07266.
OMAiCSHLDSH.
OrthoDBiEOG7X3QR9.
PhylomeDBiQ07266.
TreeFamiTF318935.

Miscellaneous databases

NextBioi615196.
PROiQ07266.

Gene expression databases

ExpressionAtlasiQ07266. baseline and differential.
GenevisibleiQ07266. RN.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028438. Drebrin.
[Graphical view]
PANTHERiPTHR10829:SF1. PTHR10829:SF1. 2 hits.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells."
    Shirao T., Obata N., Obata K.
    NeuroReport 3:109-112(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Wistar.
    Tissue: Brain and Hippocampus.
  2. "Study of processes formation in drebrin cDNA transfected fibroblast cells."
    Ren Y., Kawai-Hirai R., Xue Y., Shirao T.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM E1).
    Tissue: Embryonic brain.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; SER-342 AND SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDREB_RAT
AccessioniPrimary (citable) accession number: Q07266
Secondary accession number(s): A5D6R1, O70205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.