Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transforming growth factor beta-2

Gene

Tgfb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-2129379. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming growth factor beta-2
Short name:
TGF-beta-2
Cleaved into the following chain:
Gene namesi
Name:Tgfb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi70491. Tgfb2.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB
  • basement membrane Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • extracellular matrix Source: AgBase
  • extracellular region Source: AgBase
  • extracellular space Source: RGD
  • intracellular Source: GOC
  • neuronal cell body Source: UniProtKB
  • secretory granule Source: RGD
  • trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 330310Latency-associated peptidePRO_0000033790Add
BLAST
Chaini331 – 442112Transforming growth factor beta-2PRO_0000033791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence analysis
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
Disulfide bondi337 ↔ 346By similarity
Disulfide bondi345 ↔ 408By similarity
Disulfide bondi374 ↔ 439By similarity
Disulfide bondi378 ↔ 441By similarity
Disulfide bondi407 – 407InterchainBy similarity

Post-translational modificationi

The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ07257.

Expressioni

Tissue specificityi

Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine.1 Publication

Developmental stagei

High expression at E14. Sharp decline in expression between E16 and E18. Absent in adulthood.1 Publication

Inductioni

Both isoforms down-regulated during muscle development. Up-regulated after denervation.1 Publication

Gene expression databases

ExpressionAtlasiQ07257. baseline and differential.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with ASPN and the serine proteases, HTRA1 and HTRA3. Latency-associated peptide interacts with NREP; the interaction results in a decrease in TGFB2 autoinduction. Interacts with MFAP5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003313.

Structurei

3D structure databases

ProteinModelPortaliQ07257.
SMRiQ07257. Positions 331-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOVERGENiHBG074115.
InParanoidiQ07257.
KOiK13376.
PhylomeDBiQ07257.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR016319. TGF-beta.
IPR015615. TGF-beta-rel.
IPR003940. TGFb2.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 3 hits.
PTHR11848:SF141. PTHR11848:SF141. 3 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF001787. TGF-beta. 1 hit.
PRINTSiPR01423. TGFBETA.
PR01425. TGFBETA2.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform TGF-beta2B (identifier: Q07257-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHYCVLRTFL LLHLVPVALS LSTCSTLDMD QFMRKRIEAI RGQILSKLKL
60 70 80 90 100
TSPPEDYPEP DEVPPEVISI YNSTRDLLQE KASRRAAACE RERSDEEYYA
110 120 130 140 150
KEVYKIDMPS HFPSETVCPV VTTSSGSVGS FCSIQSQVLC GYLDAIPPTF
160 170 180 190 200
YRPYFRIVRF DVSTMEKNAS NLVKAEFRVF RLQNPKARVA EQRIELYQIL
210 220 230 240 250
KSKDLTSPTQ RYIDSKVVKT RAEGEWLSFD VTDAVHEWLH HKDRNLGFKI
260 270 280 290 300
SLHCPCCTFI PSNNYIIPNK SQELEARFAG IDGTSTYASG DQKTIKSTRK
310 320 330 340 350
KSSGKTPHLL LMLLPSYRLE SQQSSRRRKR ALDAAYCFRN VQDNCCLRPL
360 370 380 390 400
YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHTK VLSLYNTINP
410 420 430 440
EASASPCCVS QDLEPLTILY YIGNTPKIEQ LSNMIVKSCK CS
Length:442
Mass (Da):50,534
Last modified:May 15, 2002 - v2
Checksum:i69C81A19CE06C253
GO
Isoform TGF-beta2A (identifier: Q07257-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     116-143: Missing.
     144-144: D → N

Show »
Length:414
Mass (Da):47,728
Checksum:i485DFC04EC0CA291
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991Y → C in CAB42003 (PubMed:11014222).Curated
Sequence conflicti310 – 3101L → P in AAD24484 (Ref. 3) Curated
Sequence conflicti343 – 3431D → H in CAB42003 (PubMed:11014222).Curated
Sequence conflicti343 – 3431D → H in CAA50723 (PubMed:8509457).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei116 – 14328Missing in isoform TGF-beta2A. 3 PublicationsVSP_006418Add
BLAST
Alternative sequencei144 – 1441D → N in isoform TGF-beta2A. 3 PublicationsVSP_006419

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153012 mRNA. Translation: AAD34159.1.
AF153013 mRNA. Translation: AAD34160.1.
AJ132718 mRNA. Translation: CAB42003.1.
AF135598 mRNA. Translation: AAD24484.1.
X71904 mRNA. Translation: CAA50723.1.
M96643 mRNA. Translation: AAA88514.1. Sequence problems.
PIRiA40699.
RefSeqiNP_112393.1. NM_031131.1. [Q07257-1]
XP_006250510.1. XM_006250448.2. [Q07257-2]
UniGeneiRn.24539.

Genome annotation databases

EnsembliENSRNOT00000087023; ENSRNOP00000074059; ENSRNOG00000002418. [Q07257-2]
GeneIDi81809.
KEGGirno:81809.
UCSCiRGD:70491. rat. [Q07257-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153012 mRNA. Translation: AAD34159.1.
AF153013 mRNA. Translation: AAD34160.1.
AJ132718 mRNA. Translation: CAB42003.1.
AF135598 mRNA. Translation: AAD24484.1.
X71904 mRNA. Translation: CAA50723.1.
M96643 mRNA. Translation: AAA88514.1. Sequence problems.
PIRiA40699.
RefSeqiNP_112393.1. NM_031131.1. [Q07257-1]
XP_006250510.1. XM_006250448.2. [Q07257-2]
UniGeneiRn.24539.

3D structure databases

ProteinModelPortaliQ07257.
SMRiQ07257. Positions 331-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003313.

Proteomic databases

PaxDbiQ07257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000087023; ENSRNOP00000074059; ENSRNOG00000002418. [Q07257-2]
GeneIDi81809.
KEGGirno:81809.
UCSCiRGD:70491. rat. [Q07257-1]

Organism-specific databases

CTDi7042.
RGDi70491. Tgfb2.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOVERGENiHBG074115.
InParanoidiQ07257.
KOiK13376.
PhylomeDBiQ07257.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-2129379. Molecules associated with elastic fibres.

Miscellaneous databases

PROiQ07257.

Gene expression databases

ExpressionAtlasiQ07257. baseline and differential.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR016319. TGF-beta.
IPR015615. TGF-beta-rel.
IPR003940. TGFb2.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 3 hits.
PTHR11848:SF141. PTHR11848:SF141. 3 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF001787. TGF-beta. 1 hit.
PRINTSiPR01423. TGFBETA.
PR01425. TGFBETA2.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The expression and structure of TGF-beta2 transcripts in rat muscles."
    Koishi K., Dalzell K.G., McLennan I.S.
    Biochim. Biophys. Acta 1492:311-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS TGF-BETA2A AND TGF-BETA2B), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: Wistar.
    Tissue: Muscle.
  2. "Transforming growth factor-beta2 mediates mesenchymal-epithelial interactions of testicular somatic cells."
    Konrad L., Albrecht M., Renneberg H., Aumuller G.
    Endocrinology 141:3679-3686(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TGF-BETA2A).
    Strain: Wistar.
  3. "Rat transforming growth factor-beta2, complete coding sequence."
    Plisov S.Y., Ivanov S.V., Plisova T.M., Lerman M., Perantoni A.O.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TGF-BETA2A).
  4. "A role for TGF-beta in oligodendrocyte differentiation."
    McKinnon R.D., Piras G., Ida J., Dubois-Dalq M.
    J. Cell Biol. 121:1397-1407(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 278-348.
  5. "Cell-cell signaling between adult rat ventricular myocytes and cardiac microvascular endothelial cells in heterotypic primary culture."
    Nishida M., Springhorn J.P., Kelly R.A., Smith T.W.
    J. Clin. Invest. 91:1934-1941(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-441.
    Strain: Sprague-Dawley.
    Tissue: Heart.

Entry informationi

Entry nameiTGFB2_RAT
AccessioniPrimary (citable) accession number: Q07257
Secondary accession number(s): Q63574
, Q9QW26, Q9R281, Q9R298, Q9R2B8, Q9WUQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 15, 2002
Last modified: June 8, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.