Q07251 (LDH_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase
EC=1.1.1.27

Gene names

Name:	ldh
Ordered Locus Names:	H16_A0666

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the LDH2/MDH2 oxidoreductase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	L-lactate dehydrogenase		PRO_0000083826

Sequences

	Sequence		Length	Mass (Da)
	Q07251 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7A8FB0E7859CBC04	FASTA	349	36,756
	10 20 30 40 50 60 MKISLTSARQ LARDILAAQQ VPADIADDVA EHLVESDRCG YISHGLSILP NYRTALDGHS 70 80 90 100 110 120 VNPQGRAKCV LDQGTLMVFD GDGGFGQHVG KSVMQAAIER VRQHGHCIVT LRRSHHLGRM 130 140 150 160 170 180 GHYGEMAAAA GFVLLSFTNV INRAPVVAPF GGRVARLTTN PLCFAGPMPN GRPPLVVDIA 190 200 210 220 230 240 TSAIAINKAR VLAEKGEPAP EGSIIGADGN PTTDASTMFG EHPGALLPFG GHKGYALGVV 250 260 270 280 290 300 AELLAGVLSG GGTIQPDNPR GGVATNNLFA VLLNPALDLG LDWQSAEVEA FVRYLHDTPP 310 320 330 340 APGVDRVQYP GEYEAANRAQ ASDTLNINPA IWRNLERLAQ SLNVAVPTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Alcaligenes eutrophus ldh structural gene encodes a novel type of lactate dehydrogenase." Jendrossek D., Kratzin H.D., Steinbuechel A. FEMS Microbiol. Lett. 112:229-235(1993) [PubMed: 8405966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22737 Genomic DNA. Translation: CAA80432.1. AM260479 Genomic DNA. Translation: CAJ91814.1.
PIR	I39535.
RefSeq	YP_725182.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q07251.
ModBase	Search...
Protein-protein interaction databases
STRING	Q07251.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4250282.
GenomeReviews	Gene locus H16_A0666 in contig AM260479_GR.
KEGG	reh:H16_A0666.
PATRIC	35230731. VBIRalEut6770_1034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2055.
HOGENOM	HBG553037.
OMA	NVINRAP.
PhylomeDB	Q07251.
ProtClustDB	CLSK908363.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A0666-MONOMER.
Family and domain databases
InterPro	IPR003767. Malate/L-lactate_DH. [Graphical view]
KO	K00016.
PANTHER	PTHR11091. MDH_LDH. 1 hit.
Pfam	PF02615. Ldh_2. 1 hit. [Graphical view]
SUPFAM	SSF89733. MDH_LDH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LDH_CUPNH

Accession

Primary (citable) accession number: Q07251
Secondary accession number(s): Q0KDV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program