Reviewed, UniProtKB/Swiss-Prot Q07250 (KCCS_MALDO)

Names and origin

Protein names	Recommended name:     Calcium/calmodulin-dependent serine/threonine-protein kinase     EC=2.7.11.17
Organism	Malus domestica (Apple) (Pyrus malus)
Taxonomic identifier	3750 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in signal transduction processes.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Calcium/calmodulin-dependent serine/threonine-protein kinase		PRO_0000086110
Regions
	Domain	12 – 308	297	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity
	Region	318 – 328	11	Calmodulin-binding By similarity
	Compositional bias	34 – 38	5	Poly-Ser
	Compositional bias	73 – 76	4	Poly-Ala
Sites
	Active site	173	1	Proton acceptor By similarity
	Binding site	46	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07250-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8DF34A4418A9A363	FASTA	415	46,492
	10 20 30 40 50 60 MIQETRRLAD EYEISEILGR GGFSVVRKGI SRKSSSSSDK TDVAIKTLKR PFAPSNPPPL 70 80 90 100 110 120 PPHARRNDQN SFAAAAFQTR KQVSISNVLL TNEILVMRKI VENVSPHPNV IDLYDVYEDE 130 140 150 160 170 180 NGVHLVLELC SGGELFDRIV KQERYSEVGA AAVVRQIAQG LAALHRSNIV HRDLKPENCL 190 200 210 220 230 240 FLDNTVDSPL KIMDFGLSSV EEFTDPVVGL FGSIDYVSPE ALSQGQVTSK SDMWALGVIL 250 260 270 280 290 300 YILLSGYPPF IAQSNRQKQQ MIMAGEFSFY EKTWKGFLCQ PKQLISSLLK VDPDKRPSAQ 310 320 330 340 350 360 ELLDHPWVVG LSAREDQMDA EIVSRLQSFN ARRKLRAAAI ASVWTSSIFL RTKKLKSLLG 370 380 390 400 410 SYDLKPDEIK NLSSHFKKIC VKGDNATLSE FRLSCKRLIQ LRLVSMSLRA EPARL

References

[1]	"A calcium/calmodulin-binding serine/threonine protein kinase homologous to the mammalian type II calcium/calmodulin-dependent protein kinase is expressed in plant cells." Watillon B., Kettmann R., Boxus P., Burny A. Plant Physiol. 101:1381-1384(1993) [PubMed: 8310066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wijcik.
[2]	"Structure of a calmodulin-binding protein kinase gene from apple." Watillon B., Kettmann R., Boxus P., Burny A. Plant Physiol. 108:847-848(1995) [PubMed: 7610182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and characterization of an apple (Malus domestica [L.] Borkh) cDNA encoding a calmodulin-binding protein domain similar to the calmodulin-binding region of mammalian CaM kinase II." Watillon B., Kettmann R., Boxus P., Burny A. Plant Sci. 81:227-235(1992) Cited for: NUCLEOTIDE SEQUENCE OF 266-391. Strain: cv. Wijcik.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17313 mRNA. Translation: CAA78961.1. Z38126 Genomic DNA. Translation: CAA86286.1.
PIR	JQ2251.
3D structure databases
SMR	Q07250. Positions 3-338.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.17. 66979.
Family and domain databases
InterPro	IPR020642. Ca-dep_prot_kinase. IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982:SF13. Ca-dep_prot_kinase. 1 hit. PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCCS_MALDO

Accession

Primary (citable) accession number: Q07250

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)