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Protein

Cholesterol side-chain cleavage enzyme, mitochondrial

Gene

cyp11a1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Increased amounts during final oocyte maturation may be responsible for the rapid increase in the follicular production of maturation-inducing hormone 17-alpha,20-beta-DP.

Catalytic activityi

Cholesterol + 6 reduced adrenodoxin + 3 O2 + 6 H+ = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.By similarity

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi458 – 4581Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. cholesterol monooxygenase (side-chain-cleaving) activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. C21-steroid hormone metabolic process Source: UniProtKB-UniPathway
  2. cholesterol metabolic process Source: UniProtKB-KW
  3. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Steroidogenesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00229.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial (EC:1.14.15.6)
Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)
Gene namesi
Name:cyp11a1
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionSequence AnalysisAdd
BLAST
Chaini40 – 514475Cholesterol side-chain cleavage enzyme, mitochondrialPRO_0000003593Add
BLAST

Expressioni

Tissue specificityi

Not found in early vitellogenic follicles, barely detected in postvitellogenic follicles and abundant in post ovulatory follicles.

Structurei

3D structure databases

ProteinModelPortaliQ07217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG051098.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07217-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMVSWSVCRS SLALPACGLP SARHNSSMPV VRQALSPDNS STVQNFSEIP
60 70 80 90 100
GLWRNGLANL YSFWKLDGFR NIHRVMVHNF NTFGPIYREK IGYYDSVNII
110 120 130 140 150
KPEMPAILFK AEGHYPKRLT VEAWTSYRDY RNRKYGVLLK NGEDWRSNRV
160 170 180 190 200
ILNREVISPK VLGNFVPLLD EVGQDFVARV HKKIERSGQD KWTTDLSQEL
210 220 230 240 250
FKYALESVGS VLYGERLGLM LDYINPEAQH FIDCISLMFK TTSPMLYIPP
260 270 280 290 300
AMLRRVGAKI WRDHVEAWDG IFNQADRCIQ NIYRTMRQDT NTHGKYPGVL
310 320 330 340 350
ASLLMLDKLS IEDIKASVTE LMAGGVDTTS ITLLWTLYEL ARHPDLQEEL
360 370 380 390 400
RAEVAVARQS TQGDMLQMLK MIPLVKGALK ETLRLHPVAV SLQRYITEEI
410 420 430 440 450
VIQNYHIPCG TLVQLGLYAM GRDPDVFPRP EKYLPSRWLR TENQYFRSLG
460 470 480 490 500
FGFGPRQCLG RRIAETEMQL FLIHMLENFR VDKQRQVEVH STFELILLPE
510
KPILLTLKPL KSGQ
Length:514
Mass (Da):59,137
Last modified:October 1, 1994 - v1
Checksum:i0C6F49ECE8FF38FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57305 mRNA. Translation: AAB25804.1.
PIRiS32197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57305 mRNA. Translation: AAB25804.1.
PIRiS32197.

3D structure databases

ProteinModelPortaliQ07217.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG051098.

Enzyme and pathway databases

UniPathwayiUPA00229.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis."
    Takahashi M., Tanaka M., Sakai N., Adachi S., Miller W.L., Nagahama Y.
    FEBS Lett. 319:45-48(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiCP11A_ONCMY
AccessioniPrimary (citable) accession number: Q07217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.