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Protein

Eukaryotic translation initiation factor 5

Gene

Eif5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPSequence analysis8

GO - Molecular functioni

  • cadherin binding Source: RGD
  • eukaryotic initiation factor eIF2 binding Source: RGD
  • GTP binding Source: UniProtKB-KW
  • RNA binding Source: RGD
  • translation initiation factor activity Source: RGD

GO - Biological processi

  • activation of GTPase activity Source: RGD
  • formation of translation preinitiation complex Source: RGD
  • regulation of translational initiation Source: RGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-72702 Ribosomal scanning and start codon recognition
R-RNO-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:Eif5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi619861 Eif5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125181 – 429Eukaryotic translation initiation factor 5Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei225PhosphothreonineBy similarity1
Modified residuei227PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1 Publication1
Modified residuei388PhosphoserineCombined sources1 Publication1
Modified residuei408PhosphoserineBy similarity1
Cross-linki411Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki416Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei417PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07205
PRIDEiQ07205

PTM databases

iPTMnetiQ07205
PhosphoSitePlusiQ07205
SwissPalmiQ07205

Expressioni

Gene expression databases

BgeeiENSRNOG00000010218
GenevisibleiQ07205 RN

Interactioni

Subunit structurei

Interacts with FMR1; this interaction occurs in a RNA-dependent manner.By similarity

GO - Molecular functioni

  • cadherin binding Source: RGD
  • eukaryotic initiation factor eIF2 binding Source: RGD

Protein-protein interaction databases

BioGridi2485841 interactor.
STRINGi10116.ENSRNOP00000013695

Structurei

3D structure databases

ProteinModelPortaliQ07205
SMRiQ07205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini231 – 390W2PROSITE-ProRule annotationAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi194 – 200Asp/Glu-rich (highly acidic)7
Compositional biasi382 – 400Asp/Glu-rich (highly acidic)Add BLAST19
Compositional biasi421 – 427Asp-rich (acidic)7

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Phylogenomic databases

eggNOGiKOG2767 Eukaryota
COG1601 LUCA
GeneTreeiENSGT00390000016478
HOGENOMiHOG000214327
HOVERGENiHBG006132
InParanoidiQ07205
KOiK03262
OMAiDDKFYRY
OrthoDBiEOG091G0AKW
PhylomeDBiQ07205

Family and domain databases

Gene3Di1.25.40.1801 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR016021 MIF4-like_sf
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
IPR003307 W2_domain
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SM00515 eIF5C, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF48371 SSF48371, 1 hit
SSF75689 SSF75689, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit

Sequencei

Sequence statusi: Complete.

Q07205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP
60 70 80 90 100
TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP
110 120 130 140 150
ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD
160 170 180 190 200
IGTGKKEKEK KNRKGKDKEN GSVSTSETPP PPPPNEISPP HAVEEEEDDD
210 220 230 240 250
WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI LFDFVKKKKE
260 270 280 290 300
EGIIDSSDKD IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR
310 320 330 340 350
FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII
360 370 380 390 400
SWSEKASKKY VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE
410 420
VVYSKTASVP KVETVKSDNK DDDIDIDAI
Length:429
Mass (Da):48,954
Last modified:October 1, 1994 - v1
Checksum:iB1A62E30936908EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11651 mRNA Translation: AAA41112.1
BC062398 mRNA Translation: AAH62398.1
PIRiA47305
RefSeqiNP_001316808.1, NM_001329879.1
NP_064460.1, NM_020075.1
XP_006240671.1, XM_006240609.3
XP_017458786.1, XM_017603297.1
UniGeneiRn.40123

Genome annotation databases

EnsembliENSRNOT00000013695; ENSRNOP00000013695; ENSRNOG00000010218
GeneIDi108348073
56783
KEGGirno:108348073
rno:56783
UCSCiRGD:619861 rat

Similar proteinsi

Entry informationi

Entry nameiIF5_RAT
AccessioniPrimary (citable) accession number: Q07205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome