Reviewed, UniProtKB/Swiss-Prot Q07204 (F16P1_BRANA)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, chloroplastic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

Name:

FBP

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast stroma.
Induction	Light activation through pH changes, Mg2+ levels and also by light-modulated reduction of essential disulfide groups via the ferredoxin-thioredoxin f system By similarity.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Chloroplast By similarity
	Chain	54 – 411	358	Fructose-1,6-bisphosphatase, chloroplastic		PRO_0000008815
Regions
	Region	186 – 189	4	Substrate binding By similarity
Sites
	Metal binding	133	1	Magnesium 1 By similarity
	Metal binding	162	1	Magnesium 1 By similarity
	Metal binding	162	1	Magnesium 2 By similarity
	Metal binding	183	1	Magnesium 2 By similarity
	Metal binding	183	1	Magnesium 3 By similarity
	Metal binding	185	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	186	1	Magnesium 3 By similarity
	Metal binding	359	1	Magnesium 3 By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	323	1	Substrate By similarity
	Binding site	341	1	Substrate By similarity
	Binding site	343	1	Substrate By similarity
	Binding site	353	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	227 ↔ 232		Redox-active (light-modulated) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07204-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 83AC46D39EA7CECF	FASTA	411	44,446
	10 20 30 40 50 60 MAATAGATPS SHLLLSSSRH VAASPQPRIL FPSLSGKRVA VGKNHHATGV RCMAVAADAT 70 80 90 100 110 120 AETKPAAKKK SGYELQTLTS WLLRQEMKGE IDTELTIVMS SIAMACKQIA SLVQRAGISN 130 140 150 160 170 180 LTGVQGAVNI QGEDQKKLDV VSNEVFSNCL RSSGRTGIIA SEEEDVPVAV EESYSGNYVV 190 200 210 220 230 240 VFDPLDGSSN IDAAVSTGSI FGIYSPNDEC LPDSDDTSAL GSEEERCIVN VCQPGNNLLA 250 260 270 280 290 300 AGYCMYSSSV IFVLTLGKGV FAFTLDPMYG EFVLTQENIE IPKAGKIYSF NEGNYQMWDE 310 320 330 340 350 360 NLKKYIDDLK DPGPSGKPYS ARYIGSLVGD FHRTLLYGGI YGYPRDAKSK NGKLRLLYEC 370 380 390 400 410 APMSFIVEQA GGKGSDGHHR VLDIQPTEIH QRVPLYIGSK EEVEKLEKYL A

References

[1]	"Sequence of a cDNA encoding chloroplast fructose-1,6-bisphosphatase from rapeseed." Rodriguez-Suarez R.J., Wolosiuk R.A. Plant Physiol. 103:1453-1454(1993) [PubMed: 8290637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L15303 mRNA. Translation: AAB88708.1.
PIR	T07987.
3D structure databases
HSSP	HSSP built from PDB template 1D9Q based on UniProtKB P46275.
SMR	Q07204. Positions 73-411.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.11. 393.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR017955. IMPase/FBPase. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. FBPHPHTASE. PR00377. INFBPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P1_BRANA

Accession

Primary (citable) accession number: Q07204

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)