Q071E0 (SET8A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-lysine methyltransferase SETD8-A
EC=2.1.1.-

Alternative name(s):
Histone-lysine N-methyltransferase SETD8-A
EC=2.1.1.43
SET domain-containing protein 8-A

Gene names

Name:	setd8a
Synonyms:	set8a, setd8
ORF Names:	zgc:153719

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus By similarity. Chromosome By similarity. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Transcription Transcription regulation
Cellular component	Chromosome Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Repressor Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 21282610. Source: ZFIN mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of gene expression Inferred from mutant phenotype PubMed 21282610. Source: ZFIN regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histone-lysine N-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	N-lysine methyltransferase SETD8-A		PRO_0000316999
Regions
	Domain	207 – 333	127	SET
	Region	218 – 220	3	S-adenosyl-L-methionine binding By similarity
	Region	290 – 291	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	263	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	16	1	D → E in AAI22397. Ref.2
	Sequence conflict	89	1	V → A in AAI22397. Ref.2
	Sequence conflict	100	1	P → R in AAI22397. Ref.2
	Sequence conflict	145	1	S → P in AAI22397. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q071E0 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 93FE1D36C3AEF976	FASTA	344	39,155
	10 20 30 40 50 60 MFPAVNEIKV QTWRRDQRGE CPRRSAQCRL FSCAHRECAP AARSLENRGP ENRTARPEAT 70 80 90 100 110 120 MSGDVLCNGH SAFHRLLKHS SSRTHEALVV KIIQENKNTP LFCTAPEPRR DSGVNGEFLL 130 140 150 160 170 180 NSAELQDEQT LPLHCIHSAA DITSSKHRKP ARRKVKRSTK RAAESKSPAN RKVTDYFPIR 190 200 210 220 230 240 RSSRKSKSEL KYEEKQHIDT LISNGIEDGM MVRFIEGKGR GVFATQPFQK GQYVVEYHGD 250 260 270 280 290 300 LLQITDAKQR EALYAQDPST GCYMYYFQYL SKTYCVDATK ESDRLGRLIN HSKNGNCQTK 310 320 330 340 LHAIAGKPHL ILVASRDIQE GEELLYDYGD RSKSSIEAHP WLKH

References

[1]	"Hematopoietic gene expression profile in zebrafish kidney marrow." Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney marrow.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ343297 mRNA. Translation: ABC68593.1. BC122396 mRNA. Translation: AAI22397.1.
IPI	IPI00804486.
RefSeq	NP_001038814.2. NM_001045349.2.
UniGene	Dr.122337. Dr.77877.
3D structure databases
ProteinModelPortal	Q071E0.
SMR	Q071E0. Positions 184-344.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000089529.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	751629.
KEGG	dre:751629.
Organism-specific databases
CTD	751629.
ZFIN	ZDB-GENE-060825-37. setd8a.
Phylogenomic databases
eggNOG	COG2940.
HOGENOM	HOG000020818.
KO	K11428.
OrthoDB	EOG415GFP.
Family and domain databases
InterPro	IPR016858. Hist_H4-K20_MeTrfase. IPR001214. SET_dom. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
PIRSF	PIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
SMART	SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20917781.

Entry information

Entry name

SET8A_DANRE

Accession

Primary (citable) accession number: Q071E0
Secondary accession number(s): Q0P3X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program