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Protein

N-lysine methyltransferase SETD8-A

Gene

setd8a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei263 – 2631S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • dorsal/ventral pattern formation Source: ZFIN
  • histone lysine methylation Source: GOC
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of gene expression Source: ZFIN
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
N-lysine methyltransferase SETD8-A (EC:2.1.1.-)
Alternative name(s):
Histone-lysine N-methyltransferase SETD8-A (EC:2.1.1.43)
SET domain-containing protein 8-A
Gene namesi
Name:setd8a
Synonyms:set8a, setd8
ORF Names:zgc:153719
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-060825-37. setd8a.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity

  • Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344N-lysine methyltransferase SETD8-APRO_0000316999Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi591485. 1 interaction.
STRINGi7955.ENSDARP00000089529.

Structurei

3D structure databases

ProteinModelPortaliQ071E0.
SMRiQ071E0. Positions 184-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 329122SETPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2203S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni290 – 2912S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2940.
HOGENOMiHOG000020818.
InParanoidiQ071E0.
KOiK11428.
PhylomeDBiQ071E0.

Family and domain databases

InterProiIPR016858. Hist_H4-K20_MeTrfase.
IPR001214. SET_dom.
[Graphical view]
PANTHERiPTHR22884:SF11. PTHR22884:SF11. 1 hit.
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51571. SAM_MT43_PR_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q071E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPAVNEIKV QTWRRDQRGE CPRRSAQCRL FSCAHRECAP AARSLENRGP
60 70 80 90 100
ENRTARPEAT MSGDVLCNGH SAFHRLLKHS SSRTHEALVV KIIQENKNTP
110 120 130 140 150
LFCTAPEPRR DSGVNGEFLL NSAELQDEQT LPLHCIHSAA DITSSKHRKP
160 170 180 190 200
ARRKVKRSTK RAAESKSPAN RKVTDYFPIR RSSRKSKSEL KYEEKQHIDT
210 220 230 240 250
LISNGIEDGM MVRFIEGKGR GVFATQPFQK GQYVVEYHGD LLQITDAKQR
260 270 280 290 300
EALYAQDPST GCYMYYFQYL SKTYCVDATK ESDRLGRLIN HSKNGNCQTK
310 320 330 340
LHAIAGKPHL ILVASRDIQE GEELLYDYGD RSKSSIEAHP WLKH
Length:344
Mass (Da):39,155
Last modified:October 31, 2006 - v1
Checksum:i93FE1D36C3AEF976
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161D → E in AAI22397 (Ref. 2) Curated
Sequence conflicti89 – 891V → A in AAI22397 (Ref. 2) Curated
Sequence conflicti100 – 1001P → R in AAI22397 (Ref. 2) Curated
Sequence conflicti145 – 1451S → P in AAI22397 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ343297 mRNA. Translation: ABC68593.1.
BC122396 mRNA. Translation: AAI22397.1.
RefSeqiNP_001038814.2. NM_001045349.2.
UniGeneiDr.122337.
Dr.77877.

Genome annotation databases

GeneIDi751629.
KEGGidre:751629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ343297 mRNA. Translation: ABC68593.1.
BC122396 mRNA. Translation: AAI22397.1.
RefSeqiNP_001038814.2. NM_001045349.2.
UniGeneiDr.122337.
Dr.77877.

3D structure databases

ProteinModelPortaliQ071E0.
SMRiQ071E0. Positions 184-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi591485. 1 interaction.
STRINGi7955.ENSDARP00000089529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi751629.
KEGGidre:751629.

Organism-specific databases

CTDi751629.
ZFINiZDB-GENE-060825-37. setd8a.

Phylogenomic databases

eggNOGiCOG2940.
HOGENOMiHOG000020818.
InParanoidiQ071E0.
KOiK11428.
PhylomeDBiQ071E0.

Miscellaneous databases

NextBioi20917781.
PROiQ071E0.

Family and domain databases

InterProiIPR016858. Hist_H4-K20_MeTrfase.
IPR001214. SET_dom.
[Graphical view]
PANTHERiPTHR22884:SF11. PTHR22884:SF11. 1 hit.
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51571. SAM_MT43_PR_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney marrow.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.

Entry informationi

Entry nameiSET8A_DANRE
AccessioniPrimary (citable) accession number: Q071E0
Secondary accession number(s): Q0P3X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 31, 2006
Last modified: April 1, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.