Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q071E0 (SET8A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-lysine methyltransferase SETD8-A

EC=2.1.1.-
Alternative name(s):
Histone-lysine N-methyltransferase SETD8-A
EC=2.1.1.43
SET domain-containing protein 8-A
Gene names
Name:setd8a
Synonyms:set8a, setd8
ORF Names:zgc:153719
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus By similarity. Chromosome By similarity. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.

Contains 1 SET domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344N-lysine methyltransferase SETD8-A
PRO_0000316999

Regions

Domain208 – 329122SET
Region218 – 2203S-adenosyl-L-methionine binding By similarity
Region290 – 2912S-adenosyl-L-methionine binding By similarity

Sites

Binding site2631S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict161D → E in AAI22397. Ref.2
Sequence conflict891V → A in AAI22397. Ref.2
Sequence conflict1001P → R in AAI22397. Ref.2
Sequence conflict1451S → P in AAI22397. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q071E0 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 93FE1D36C3AEF976

FASTA34439,155
        10         20         30         40         50         60 
MFPAVNEIKV QTWRRDQRGE CPRRSAQCRL FSCAHRECAP AARSLENRGP ENRTARPEAT 

        70         80         90        100        110        120 
MSGDVLCNGH SAFHRLLKHS SSRTHEALVV KIIQENKNTP LFCTAPEPRR DSGVNGEFLL 

       130        140        150        160        170        180 
NSAELQDEQT LPLHCIHSAA DITSSKHRKP ARRKVKRSTK RAAESKSPAN RKVTDYFPIR 

       190        200        210        220        230        240 
RSSRKSKSEL KYEEKQHIDT LISNGIEDGM MVRFIEGKGR GVFATQPFQK GQYVVEYHGD 

       250        260        270        280        290        300 
LLQITDAKQR EALYAQDPST GCYMYYFQYL SKTYCVDATK ESDRLGRLIN HSKNGNCQTK 

       310        320        330        340 
LHAIAGKPHL ILVASRDIQE GEELLYDYGD RSKSSIEAHP WLKH 

« Hide

References

[1]"Hematopoietic gene expression profile in zebrafish kidney marrow."
Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z.
Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney marrow.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ343297 mRNA. Translation: ABC68593.1.
BC122396 mRNA. Translation: AAI22397.1.
RefSeqNP_001038814.2. NM_001045349.2.
UniGeneDr.122337.
Dr.77877.

3D structure databases

ProteinModelPortalQ071E0.
SMRQ071E0. Positions 184-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid591485. 1 interaction.
STRING7955.ENSDARP00000089529.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID751629.
KEGGdre:751629.

Organism-specific databases

CTD751629.
ZFINZDB-GENE-060825-37. setd8a.

Phylogenomic databases

eggNOGCOG2940.
HOGENOMHOG000020818.
KOK11428.
PhylomeDBQ071E0.

Family and domain databases

InterProIPR016858. Hist_H4-K20_MeTrfase.
IPR001214. SET_dom.
[Graphical view]
PfamPF00856. SET. 1 hit.
[Graphical view]
PIRSFPIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
SMARTSM00317. SET. 1 hit.
[Graphical view]
PROSITEPS51571. SAM_MT43_PR_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20917781.
PROQ071E0.

Entry information

Entry nameSET8A_DANRE
AccessionPrimary (citable) accession number: Q071E0
Secondary accession number(s): Q0P3X5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 31, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families