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Q071E0

- SET8A_DANRE

UniProt

Q071E0 - SET8A_DANRE

Protein

N-lysine methyltransferase SETD8-A

Gene

setd8a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei263 – 2631S-adenosyl-L-methioninePROSITE-ProRule annotation

    GO - Molecular functioni

    1. histone-lysine N-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. dorsal/ventral pattern formation Source: ZFIN
    2. histone lysine methylation Source: GOC
    3. mitotic nuclear division Source: UniProtKB-KW
    4. positive regulation of gene expression Source: ZFIN
    5. regulation of transcription, DNA-templated Source: UniProtKB-KW
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Repressor, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-lysine methyltransferase SETD8-A (EC:2.1.1.-)
    Alternative name(s):
    Histone-lysine N-methyltransferase SETD8-A (EC:2.1.1.43)
    SET domain-containing protein 8-A
    Gene namesi
    Name:setd8a
    Synonyms:set8a, setd8
    ORF Names:zgc:153719
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-060825-37. setd8a.

    Subcellular locationi

    Nucleus By similarity. Chromosome By similarity
    Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms By similarity.By similarity

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344N-lysine methyltransferase SETD8-APRO_0000316999Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi591485. 1 interaction.
    STRINGi7955.ENSDARP00000089529.

    Structurei

    3D structure databases

    ProteinModelPortaliQ071E0.
    SMRiQ071E0. Positions 184-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini208 – 329122SETPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2203S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni290 – 2912S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.PROSITE-ProRule annotation
    Contains 1 SET domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2940.
    HOGENOMiHOG000020818.
    KOiK11428.
    PhylomeDBiQ071E0.

    Family and domain databases

    InterProiIPR016858. Hist_H4-K20_MeTrfase.
    IPR001214. SET_dom.
    [Graphical view]
    PfamiPF00856. SET. 1 hit.
    [Graphical view]
    PIRSFiPIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
    SMARTiSM00317. SET. 1 hit.
    [Graphical view]
    PROSITEiPS51571. SAM_MT43_PR_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q071E0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPAVNEIKV QTWRRDQRGE CPRRSAQCRL FSCAHRECAP AARSLENRGP    50
    ENRTARPEAT MSGDVLCNGH SAFHRLLKHS SSRTHEALVV KIIQENKNTP 100
    LFCTAPEPRR DSGVNGEFLL NSAELQDEQT LPLHCIHSAA DITSSKHRKP 150
    ARRKVKRSTK RAAESKSPAN RKVTDYFPIR RSSRKSKSEL KYEEKQHIDT 200
    LISNGIEDGM MVRFIEGKGR GVFATQPFQK GQYVVEYHGD LLQITDAKQR 250
    EALYAQDPST GCYMYYFQYL SKTYCVDATK ESDRLGRLIN HSKNGNCQTK 300
    LHAIAGKPHL ILVASRDIQE GEELLYDYGD RSKSSIEAHP WLKH 344
    Length:344
    Mass (Da):39,155
    Last modified:October 31, 2006 - v1
    Checksum:i93FE1D36C3AEF976
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161D → E in AAI22397. 1 PublicationCurated
    Sequence conflicti89 – 891V → A in AAI22397. 1 PublicationCurated
    Sequence conflicti100 – 1001P → R in AAI22397. 1 PublicationCurated
    Sequence conflicti145 – 1451S → P in AAI22397. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ343297 mRNA. Translation: ABC68593.1.
    BC122396 mRNA. Translation: AAI22397.1.
    RefSeqiNP_001038814.2. NM_001045349.2.
    UniGeneiDr.122337.
    Dr.77877.

    Genome annotation databases

    GeneIDi751629.
    KEGGidre:751629.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ343297 mRNA. Translation: ABC68593.1 .
    BC122396 mRNA. Translation: AAI22397.1 .
    RefSeqi NP_001038814.2. NM_001045349.2.
    UniGenei Dr.122337.
    Dr.77877.

    3D structure databases

    ProteinModelPortali Q071E0.
    SMRi Q071E0. Positions 184-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 591485. 1 interaction.
    STRINGi 7955.ENSDARP00000089529.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 751629.
    KEGGi dre:751629.

    Organism-specific databases

    CTDi 751629.
    ZFINi ZDB-GENE-060825-37. setd8a.

    Phylogenomic databases

    eggNOGi COG2940.
    HOGENOMi HOG000020818.
    KOi K11428.
    PhylomeDBi Q071E0.

    Miscellaneous databases

    NextBioi 20917781.
    PROi Q071E0.

    Family and domain databases

    InterProi IPR016858. Hist_H4-K20_MeTrfase.
    IPR001214. SET_dom.
    [Graphical view ]
    Pfami PF00856. SET. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
    SMARTi SM00317. SET. 1 hit.
    [Graphical view ]
    PROSITEi PS51571. SAM_MT43_PR_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney marrow.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.

    Entry informationi

    Entry nameiSET8A_DANRE
    AccessioniPrimary (citable) accession number: Q071E0
    Secondary accession number(s): Q0P3X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3