Reviewed,
UniProtKB/Swiss-Prot Q07192 (MP2K2_XENLA)
Last modified
June 16, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
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Names and origin
| Protein names | Recommended name: Dual specificity mitogen-activated protein kinase kinase 2 Short name=MAP kinase kinase 2 Short name=MAPKK 2 EC=2.7.12.2 Alternative name(s): MAPK-ERK kinase 2 | ||||
| Gene names |
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| Organism | Xenopus laevis (African clawed frog) | ||||
| Taxonomic identifier | 8355 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Protein attributes
| Sequence length | 446 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Tissue specificity | Expressed abundantly in the adult brain and muscle. |
| Developmental stage | It is initially expressed in the dorsal region of the embryo in a diffuse pattern at stage 17. Subsequently expression occurs in the early stages of development of the central nervous system, including the brain, spinal cord and eye. Later (stage 24) expression is found in the hindbrain, midbrain and forebrain and the somites. By stage 32, the expression is detected in the cranial neurons. Expression in the brain is increased, while expression in the spinal cord has decreased by stage 37. |
| Post-translational modification | MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases. |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q07192-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q07192-2) The sequence of this isoform differs from the canonical sequence as follows: 51-51: G → GLQINLCDNTQS | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | ‹1 – 446 | ›446 | Dual specificity mitogen-activated protein kinase kinase 2 | PRO_0000086377 | |||||
Regions | |||||||||
| Domain | 149 – 414 | 266 | Protein kinase | ||||||
| Nucleotide binding | 155 – 163 | 9 | ATP By similarity | ||||||
| Compositional bias | 22 – 40 | 19 | Ser-rich | ||||||
Sites | |||||||||
| Active site | 276 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 178 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 304 | 1 | Phosphoserine; by RAF By similarity | ||||||
| Modified residue | 308 | 1 | Phosphothreonine; by RAF By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 51 | 1 | G → GLQINLCDNTQS in isoform 2. | VSP_004876 | |||||
Experimental info | |||||||||
| Mutagenesis | 178 | 1 | K → R: Loss of activity. | ||||||
| Non-terminal residue | 1 | 1 | |||||||
Sequences
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References
| [1] | "Novel members of the mitogen-activated protein kinase activator family in Xenopus laevis." Yashar B.M., Kelley C., Yee K., Errede B., Zon L.I. Mol. Cell. Biol. 13:5738-5748(1993) [PubMed: 8395011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Embryo. |
Cross-references
Sequence databases | |
|---|---|
| Z22736 mRNA. Translation: CAA80430.1. | |
| PIR | A54694. S36039. |
| UniGene | Xl.13308 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1F3M based on UniProtKB Q13153. |
| ModBase | Search... |
Organism-specific databases | |
| Xenbase | XB-FEAT-482150. map2k4. |
Phylogenomic databases | |
| HOVERGEN | Q07192. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.2. 648. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MP2K2_XENLA | ||||||||
| Accession | Primary (citable) accession number: Q07192 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Xenopus annotation project | ||||||||
