Reviewed,
UniProtKB/Swiss-Prot Q07174 (M3K8_MOUSE)
Last modified
January 19, 2010.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase kinase kinase 8 EC=2.7.11.25 Alternative name(s): COT proto-oncogene serine/threonine-protein kinase Cancer Osaka thyroid oncogene Short name=C-COT | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 467 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Subunit structure | Forms a ternary complex with NFKB1 and TNIP2 By similarity. |
| Subcellular location | |
| Tissue specificity | High in adult submandibular gland, thymus, spleen and newborn digestive tract. |
| Post-translational modification | Autophosphorylated By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Disease | Proto-oncogene |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | cell cycle Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| RIPK1 | Q13546 | 1 | EBI-309771,EBI-358507 | From a different organism. |
| Ripk1 | Q60855 | 1 | EBI-309771,EBI-529119 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 467 | 467 | Mitogen-activated protein kinase kinase kinase 8 | PRO_0000086256 | |||||
Regions | |||||||||
| Domain | 138 – 388 | 251 | Protein kinase | ||||||
| Nucleotide binding | 144 – 152 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 253 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 167 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 141 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | "The murine cot proto-oncogene: genome structure and tissue-specific expression." Ohara R., Miyoshi J., Aoki M., Toyoshima K. Jpn. J. Cancer Res. 84:518-525(1993) [PubMed: 8320169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D13759 mRNA. Translation: BAA02905.1. |
| IPI | IPI00308992. |
| PIR | I49609. |
| RefSeq | NP_031772.1. |
| UniGene | Mm.3275 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q07174. 5 interactions. |
| STRING | Q07174. |
PTM databases | |
| PhosphoSite | Q07174. |
Proteomic databases | |
| PRIDE | Q07174. |
Genome annotation databases | |
| Ensembl | ENSMUST00000025078; ENSMUSP00000025078; ENSMUSG00000024235; Mus musculus. [Genome view] |
| GeneID | 26410. |
| KEGG | mmu:26410. |
| UCSC | uc008dye.1. mouse. |
Organism-specific databases | |
| CTD | 26410. |
| MGI | MGI:1346878. Map3k8. |
Phylogenomic databases | |
| eggNOG | roNOG09026. |
| HOGENOM | HBG717754. |
| HOVERGEN | Q07174. |
| InParanoid | Q07174. |
| OMA | DCSPGMR. |
| OrthoDB | EOG95B41S. |
| PhylomeDB | Q07174. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.25. 244. |
Gene expression databases | |
| ArrayExpress | Q07174. |
| Bgee | Q07174. |
| CleanEx | MM_MAP3K8. |
| Genevestigator | Q07174. |
| GermOnline | ENSMUSG00000024235. Mus musculus. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR017424. MAPKKK8. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PIRSF | PIRSF038171. MAPKKK8. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 304397. |
| SOURCE | Search... |
Entry information
| Entry name | M3K8_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q07174 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


