ID   ALF1_STACT              Reviewed;         296 AA.
AC   Q07159; B9DJW7;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Fructose-bisphosphate aldolase class 1;
DE            EC=4.1.2.13;
DE   AltName: Full=Fructose-biphosphate aldolase class I;
DE            Short=FBP aldolase;
GN   Name=fda; OrderedLocusNames=Sca_2144;
OS   Staphylococcus carnosus (strain TM300).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=396513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-8.
RX   MEDLINE=94042930; PubMed=8226699;
RA   Witke C., Goetz F.;
RT   "Cloning, sequencing, and characterization of the gene encoding the
RT   class I fructose-1,6-bisphosphate aldolase of Staphylococcus
RT   carnosus.";
RL   J. Bacteriol. 175:7495-7499(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=19060169; DOI=10.1128/AEM.01982-08;
RA   Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G.,
RA   Schuster S.C., Goetz F.;
RT   "Genome analysis of the meat starter culture bacterium Staphylococcus
RT   carnosus TM300.";
RL   Appl. Environ. Microbiol. 75:811-822(2009).
CC   -!- CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 4/4.
CC   -!- SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase
CC       family.
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DR   EMBL; X71729; CAA50663.1; -; Genomic_DNA.
DR   EMBL; AM295250; CAL29049.1; -; Genomic_DNA.
DR   PIR; A49943; A49943.
DR   RefSeq; YP_002635234.1; -.
DR   HSSP; P79226; 1FDJ.
DR   GeneID; 7551079; -.
DR   GenomeReviews; AM295250_GR; Sca_2144.
DR   BRENDA; 4.1.2.13; 3303.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   HAMAP; MF_00729; -; 1.
DR   InterPro; IPR000741; Aldolase_I.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF00274; Glycolytic; 1.
DR   ProDom; PD001128; Aldolase_I; 1.
DR   PROSITE; PS00158; ALDOLASE_CLASS_I; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; Glycolysis; Lyase;
KW   Schiff base.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    296       Fructose-bisphosphate aldolase class 1.
FT                                /FTId=PRO_0000216909.
FT   ACT_SITE    175    175       Proton acceptor (By similarity).
FT   ACT_SITE    212    212       Schiff-base intermediate with
FT                                dihydroxyacetone-P (By similarity).
SQ   SEQUENCE   296 AA;  32851 MW;  13B5033BE67BF7E9 CRC64;
     MNQEQFDKIK NGKGFIAALD QSGGSTPKAL KDYGVEENEY SNDEEMFNLV HDMRTRIITS
     PAFNGEKILG AILFEQTMDR EVEGKYTGSY LADKGIVPFL KVDKGLAEEA DGVQLMKPIP
     DLDKLLDRAN ERGIFGTKMR SNILENNKEA IEKVVKQQFE VAKEIIAAGL VPIIEPEVNI
     NAKDKEAIEA NLAEAIKAEL DNLKKDQYVM LKLTIPTKVN AYSELIEHPQ VIRVVALSGG
     YSRDEANKIL KQNDGLIASF SRALVSDLNA QQSDAEFNEK LQEAIDTIFD ASVNKA
//