Reviewed, UniProtKB/Swiss-Prot Q07159 (ALF1_STACT)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase class 1
    EC=4.1.2.13
Alternative name(s):
    Fructose-biphosphate aldolase class I
      Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	Sca_2144

Organism

Staphylococcus carnosus (strain TM300) [Complete proteome] [HAMAP]

Taxonomic identifier

396513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 296	295	Fructose-bisphosphate aldolase class 1 HAMAP MF_00729		PRO_0000216909
Sites
	Active site	175	1	Proton acceptor By similarity
	Active site	212	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07159-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 13B5033BE67BF7E9	FASTA	296	32,851
	10 20 30 40 50 60 MNQEQFDKIK NGKGFIAALD QSGGSTPKAL KDYGVEENEY SNDEEMFNLV HDMRTRIITS 70 80 90 100 110 120 PAFNGEKILG AILFEQTMDR EVEGKYTGSY LADKGIVPFL KVDKGLAEEA DGVQLMKPIP 130 140 150 160 170 180 DLDKLLDRAN ERGIFGTKMR SNILENNKEA IEKVVKQQFE VAKEIIAAGL VPIIEPEVNI 190 200 210 220 230 240 NAKDKEAIEA NLAEAIKAEL DNLKKDQYVM LKLTIPTKVN AYSELIEHPQ VIRVVALSGG 250 260 270 280 290 YSRDEANKIL KQNDGLIASF SRALVSDLNA QQSDAEFNEK LQEAIDTIFD ASVNKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and characterization of the gene encoding the class I fructose-1,6-bisphosphate aldolase of Staphylococcus carnosus." Witke C., Goetz F. J. Bacteriol. 175:7495-7499(1993) [PubMed: 8226699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8.
[2]	"Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300." Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F. Appl. Environ. Microbiol. 75:811-822(2009) [PubMed: 19060169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71729 Genomic DNA. Translation: CAA50663.1. AM295250 Genomic DNA. Translation: CAL29049.1.
PIR	A49943.
RefSeq	YP_002635234.1.
3D structure databases
HSSP	HSSP built from PDB template 1FDJ based on UniProtKB P79226.
ModBase	Search...
Genome annotation databases
GeneID	7551079.
GenomeReviews	Gene locus Sca_2144 in contig AM295250_GR.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	4.1.2.13. 3303.
Family and domain databases
HAMAP	MF_00729. [Tree]
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_STACT

Accession

Primary (citable) accession number: Q07159
Secondary accession number(s): B9DJW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)