Q07158 (TPS1_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase

Gene names

Name:	TPS1
Synonyms:	GGS1
Ordered Locus Names:	KLLA0B08822g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Appears to play a role in controlling and restricting the influx of sugar into glycolysis, probably at the level of hexokinase. Necessary for trehalose-6-phosphate synthase and phosphatase activities.
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Subunit structure	Trehalose synthase/phosphatase complex contains three or four polypeptides of 56 kDa (TPS1), 102 kDa (TPS2), 115 kDa (TPS3) and 123 kDa (TSL1).
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit		PRO_0000122498

Sequences

	Sequence		Length	Mass (Da)
	Q07158 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D616654C166B3C17	FASTA	488	55,356
	10 20 30 40 50 60 MVNQDISKLS LNECPGSVIV ISNRLPVTIK KDEKTGEYEY SMSSGGLVTA LQGLKKSTTF 70 80 90 100 110 120 QWYGWPGLEV PDEDKAKVKR ELLEKFNAIP IFLSDEVADL HYNGFSNSIL WPLFHYHPGE 130 140 150 160 170 180 ITFDDTAWLA YNEANMAFAD EIEGNINDND VVWVHDYHLM LLPEMIRQRV IAKKLKNIKI 190 200 210 220 230 240 GWFLHTPFPS SEIYRILPVR QEILKGVLSC DLIGFHTYDY ARHFLSAVQR ILNVNTLPNG 250 260 270 280 290 300 VEFDGRFVNV GAFPIGIDVE TFTEGLKQDA VIKRIKELKE SFKGCKIIIG VDRLDYIKGV 310 320 330 340 350 360 PQKLHALEVF LGAHPEWIGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR INGQFGTAEF 370 380 390 400 410 420 VPIHFMHRSI PFQELISLYA VSDVCLVSST RDGMNLVSYE YISCQEEKKG TLILSEFTGA 430 440 450 460 470 480 AQSLNGALIV NPWNTDDLAE SINEALTVPE EKRAANWEKL YKYISKYTSA FWGENFVHEL YRLGSSNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake." Luyten K., de Koning W., Tesseur I., Ruiz M.C., Ramos J., Cobbaert P., Thevelein J.M., Hohmann S. Eur. J. Biochem. 217:701-713(1993) [PubMed: 8223613] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72499 Genomic DNA. Translation: CAA51164.1. CR382122 Genomic DNA. Translation: CAH02314.1.
PIR	S38987.
RefSeq	XP_451921.1. XM_451921.1.
3D structure databases
ProteinModelPortal	Q07158.
ModBase	Search...
Protein-protein interaction databases
STRING	Q07158.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2896906.
GenomeReviews	Gene locus KLLA0B08822g in contig CR382122_GR.
KEGG	kla:KLLA0B08822g.
Phylogenomic databases
eggNOG	fuNOG04896.
HOGENOM	HBG559076.
OMA	EYGRKEM.
OrthoDB	EOG4KPXK7.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. IPR012766. Trehalose_OtsA. [Graphical view]
KO	K00697.
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
TIGRFAMs	TIGR02400. Trehalose_OtsA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPS1_KLULA

Accession

Primary (citable) accession number: Q07158

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program