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Protein

Zinc finger protein Gfi-1

Gene

Gfi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 promoter region. Implicated in the maintenance of inner ear hair cells (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri256 – 27924C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri397 – 42024C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Gfi-1
Alternative name(s):
Growth factor independent protein 1
Gene namesi
Name:Gfi1
Synonyms:Gfi-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi2680. Gfi1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with PIAS3 and RUNX1T1 in nuclear dots.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Zinc finger protein Gfi-1PRO_0000047195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201PhosphoserineCombined sources
Modified residuei57 – 571PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07120.

Expressioni

Tissue specificityi

Restricted to lymphoid tissues and testes in adult animals.1 Publication

Developmental stagei

Expression enhanced late after interaction of IL-2 with its receptor, approximately when cells enter S phase.1 Publication

Gene expression databases

GenevisibleiQ07120. RN.

Interactioni

Subunit structurei

Interacts with U2AF1L4. Component of RCOR-GFI-KDM1A-HDAC complexes. Interacts directly with RCOR1, KDM1A and HDAC2. Also interacts with HDAC1. regions. Interacts (via the zinc-finger domain) with ARIH2; the interaction prevents GFI1 ubiquitination and proteasomal degradation. Interacts with PIAS3; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Forms a complex with EHMT2 and HDAC1 to promote 'Lys-9' dimethylation of H3 (H3K9Me2) and repress expression of target genes. Interacts directly with EHMT2. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly with AJUBA (via ITS LIM domains); the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes and, occurs independent of the SNAG domain. Interacts with SPI1; the interaction inhibits SPI1 transcriptional activity and represses SPI1-regulated macrophage-specific genes required for proper granulocyte development. Interacts with RUNX1T1; the interaction represses HDAC-mediated transcriptional activity. Interacts with RELA; the interaction occurs on liposaccharide (LPS) stimulation controls RELA DNA binding activity and regulates endotoxin-mediated TOLL-like receptor inflammatory response (By similarity). Interacts (via the C-terminal zinc fingers) with ZBTB17; the interaction results in the recruitment of GFI1 to the CDKN1A/p21 promoter and repression of CDKN1A/p21 transcription.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EHMT2Q96KQ73EBI-4289236,EBI-744366From a different organism.

Protein-protein interaction databases

DIPiDIP-48689N.
IntActiQ07120. 2 interactions.
STRINGi10116.ENSRNOP00000002807.

Structurei

Secondary structure

1
423
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi316 – 3183Combined sources
Beta strandi321 – 3244Combined sources
Helixi325 – 33612Combined sources
Beta strandi344 – 3463Combined sources
Beta strandi349 – 3524Combined sources
Helixi353 – 36412Combined sources
Turni372 – 3743Combined sources
Beta strandi377 – 3804Combined sources
Helixi381 – 39111Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KMKNMR-A312-393[»]
ProteinModelPortaliQ07120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07120.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2020SNAG domainBy similarityAdd
BLAST
Regioni141 – 258118Required for interaction with RELABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi159 – 21052Ala/Gly-richAdd
BLAST

Domaini

Zinc fingers 3,4 and 5 are required for DNA binding and for interaction with SPI1.
The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors.By similarity

Sequence similaritiesi

Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri256 – 27924C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri397 – 42024C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00740000115096.
HOGENOMiHOG000067832.
HOVERGENiHBG006967.
InParanoidiQ07120.
KOiK09223.
OMAiFCERAPE.
OrthoDBiEOG72RN05.
PhylomeDBiQ07120.
TreeFamiTF350784.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR029840. GFI1/1B.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR19303:SF239. PTHR19303:SF239. 1 hit.
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE TVPVPGRADG GAVSAGESKM
60 70 80 90 100
EPRERLSPES QLTEAPDRAS ASPNSCEGSV CDPSSEFEDY WRPPSPSVSP
110 120 130 140 150
ASEKSLCRSL DEAQPYTLPF KPYAWSGLAG SDLRHLVQSY RQCSALERSA
160 170 180 190 200
GLSLFCERGA ESGRPAARYG SEQAAGGAGA GQPGSCGAAS GATSAGGLGL
210 220 230 240 250
YGDFAPAAAG LFERPSTAAG RLYQDRGHEL HADKSVGVKV ESELLCTRLL
260 270 280 290 300
LGGGSYKCIK CSKVFSTPHG LEVHVRRSHS GTRPFACEMC GKTFGHAVSL
310 320 330 340 350
EQHKAVHSQE RSFDCKICGK SFKRSSTLST HLLIHSDTRP YPCQYCGKRF
360 370 380 390 400
HQKSDMKKHT FIHTGEKPHK CQVCGKAFSQ SSNLITHSRK HTGFKPFGCD
410 420
LCGKGFQRKV DLRRHRETQH GLK
Length:423
Mass (Da):45,858
Last modified:October 1, 1994 - v1
Checksum:iFC39B6100D684D14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06986 mRNA. Translation: AAA41212.1.
PIRiA48152.
RefSeqiNP_036698.1. NM_012566.1.
XP_008768139.1. XM_008769917.1.
XP_008768140.1. XM_008769918.1.
UniGeneiRn.10935.

Genome annotation databases

EnsembliENSRNOT00000002807; ENSRNOP00000002807; ENSRNOG00000002042.
GeneIDi24388.
KEGGirno:24388.
UCSCiRGD:2680. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06986 mRNA. Translation: AAA41212.1.
PIRiA48152.
RefSeqiNP_036698.1. NM_012566.1.
XP_008768139.1. XM_008769917.1.
XP_008768140.1. XM_008769918.1.
UniGeneiRn.10935.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KMKNMR-A312-393[»]
ProteinModelPortaliQ07120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48689N.
IntActiQ07120. 2 interactions.
STRINGi10116.ENSRNOP00000002807.

Proteomic databases

PaxDbiQ07120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002807; ENSRNOP00000002807; ENSRNOG00000002042.
GeneIDi24388.
KEGGirno:24388.
UCSCiRGD:2680. rat.

Organism-specific databases

CTDi2672.
RGDi2680. Gfi1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00740000115096.
HOGENOMiHOG000067832.
HOVERGENiHBG006967.
InParanoidiQ07120.
KOiK09223.
OMAiFCERAPE.
OrthoDBiEOG72RN05.
PhylomeDBiQ07120.
TreeFamiTF350784.

Miscellaneous databases

EvolutionaryTraceiQ07120.
NextBioi603167.
PROiQ07120.

Gene expression databases

GenevisibleiQ07120. RN.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR029840. GFI1/1B.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR19303:SF239. PTHR19303:SF239. 1 hit.
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi-1) encoding a novel zinc finger protein."
    Gilks C.B., Bear S.E., Grimes H.L., Tsichlis P.N.
    Mol. Cell. Biol. 13:1759-1768(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  2. "A role of Miz-1 in Gfi-1-mediated transcriptional repression of CDKN1A."
    Liu Q., Basu S., Qiu Y., Tang F., Dong F.
    Oncogene 29:2843-2852(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZBTB17, FUNCTION.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Solution structure of Gfi-1 zinc domain bound to consensus DNA."
    Lee S., Doddapaneni K., Hogue A., McGhee L., Meyers S., Wu Z.
    J. Mol. Biol. 397:1055-1066(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 312-393 IN COMPLEX WITH DNA.

Entry informationi

Entry nameiGFI1_RAT
AccessioniPrimary (citable) accession number: Q07120
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.