Reviewed, UniProtKB/Swiss-Prot Q07116 (SUOX_RAT)

Names and origin

Protein names

Recommended name:
    Sulfite oxidase, mitochondrial
    EC=1.8.3.1

Gene names

Name:

Suox

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Sulfite + O(2) + H(2)O = sulfate + H(2)O(2).
Cofactor	Binds 1 protoheme group. Molybdenum (molybdopterin).
Pathway	Energy metabolism; sulfur metabolism.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion intermembrane space.
Sequence similarities	Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding Molybdenum
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: InterPro sulfite oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 80	80	Mitochondrion
	Chain	81 – 546	466	Sulfite oxidase, mitochondrial		PRO_0000006484
Regions
	Domain	83 – 162	80	Cytochrome b5 heme-binding
	Region	166 – 182	17	Hinge By similarity
	Region	183 – 546	364	Molybdenum-pterin domain By similarity
	Region	216 – 220	5	Molybdenum-pterin-binding By similarity
	Region	321 – 323	3	Molybdenum-pterin-binding By similarity
	Region	367 – 380	14	Molybdenum-pterin-binding By similarity
Sites
	Metal binding	119	1	Iron (heme axial ligand) By similarity
	Metal binding	144	1	Iron (heme axial ligand) By similarity
	Metal binding	265	1	Molybdenum-pterin By similarity
	Metal binding	318	1	Molybdenum-pterin By similarity
Amino acid modifications
	Modified residue	286	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	98	1	K → Q in AAA16618. Ref.2
	Sequence conflict	178	1	S → T AA sequence Ref.3
	Sequence conflict	294	1	W → R AA sequence Ref.3
	Sequence conflict	318	1	C → Q AA sequence Ref.3
	Sequence conflict	338	1	A → G AA sequence Ref.3
	Sequence conflict	362	1	H → S Ref.3 Ref.4
	Sequence conflict	394 – 396	3	SHW → HYL AA sequence Ref.3
	Sequence conflict	432	1	Q → V AA sequence Ref.3
	Sequence conflict	514	1	D → A AA sequence Ref.3
	Sequence conflict	527	1	W → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q07116-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: B4A4E46A8F15765D	FASTA	546	60,806
	10 20 30 40 50 60 MLPRLYRSVA VGLPRAIRAK STPLRLCIQA CSSSDSLKPQ HPSLTFSDDN SRTRGWKVMG 70 80 90 100 110 120 TLIGLGAVLA YHDHRCRASQ ESPRIYSKED VRSHNNLKTG VWVTLGSEVF DVTKFVDLHP 130 140 150 160 170 180 GGQSKLMLAA GGPLEPFWAL YAVHNQPHVR ELLAEYKIGE LNPEDRMSPP LEASDPYSND 190 200 210 220 230 240 PMRHPALRIN SQRPFNAEPP PELLTESYIT PNPIFFTRNH LPVPNLDPDT YRLHVVGAPG 250 260 270 280 290 300 GQSLSLSLDD LHKFPKHEVT VTLQCAGNRR SEMNKVKEVK GLEWRTGAIS TARWAGARLC 310 320 330 340 350 360 DVLAQAGHRL RETEAHVCFE GLDSDPTGTA YGASIPLARA MDPQAEVLLA YEMNGQPLPR 370 380 390 400 410 420 DHGFPVRVVV PGVVGARHVK WLGRVSVESE ESYSHWQRRD YKGFSPSVDW DTVDFDLAPS 430 440 450 460 470 480 IQELPIQSAI TQPQDGTTVE SGEVIIKGYA WSGGGRAVIR VDVSMDGGLT WQEAELEGEE 490 500 510 520 530 540 QHPRKAWAWR IWQLKAHVPA EQKELNIICK AVDDSYNVQP DTVAPIWNLR GVLSNAWHRV HVQVVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[2]	"Molecular cloning of rat liver sulfite oxidase. Expression of a eukaryotic Mo-pterin-containing enzyme in Escherichia coli." Garrett R.M., Rajagopalan K.V. J. Biol. Chem. 269:272-276(1994) [PubMed: 8276806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-546. Tissue: Liver.
[3]	"A conserved cysteine in molybdenum oxotransferases." Barber M.J., Neame P.J. J. Biol. Chem. 265:20912-20915(1990) [PubMed: 2249998] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[4]	"Sequence and nitrate regulation of the Arabidopsis thaliana mRNA encoding nitrate reductase, a metalloflavoprotein with three functional domains." Crawford N.M., Smith M., Bellissimo D.B., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 85:5006-5010(1988) [PubMed: 3393528] [Abstract] Cited for: PROTEIN SEQUENCE OF 187-201 AND 340-368. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC061991 mRNA. Translation: AAH61991.1. Different initiation. L05084 mRNA. Translation: AAA16618.1. Different initiation.
PIR	A53107.
RefSeq	NP_112389.3.
UniGene	Rn.25720
3D structure databases
HSSP	HSSP built from PDB template 1MJ4 based on UniProtKB P51687.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000005987. Rattus norvegicus. [Contig view]
GeneID	81805.
KEGG	rno:81805.
Organism-specific databases
RGD	619994. Suox.
Phylogenomic databases
HOVERGEN	Q07116.
Gene expression databases
ArrayExpress	Q07116.
GermOnline	ENSRNOG00000005987. Rattus norvegicus.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615667.

Entry information

Entry name

SUOX_RAT

Accession

Primary (citable) accession number: Q07116
Secondary accession number(s): Q6P6W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 10, 2007
Last modified:	November 25, 2008
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)