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Q07108 (CD69_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Early activation antigen CD69
Alternative name(s):
Activation inducer molecule
Short name=AIM
BL-AC/P26
C-type lectin domain family 2 member C
EA1
Early T-cell activation antigen p60
GP32/28
Leukocyte surface antigen Leu-23
MLR-3
CD_antigen=CD69
Gene names
Name:CD69
Synonyms:CLEC2C
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length199 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Subunit structure

Homodimer; disulfide-linked. Ref.6 Ref.7 Ref.8

Subcellular location

Membrane; Single-pass type II membrane protein.

Tissue specificity

Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Developmental stage

Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

Induction

By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.

Post-translational modification

Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Sequence similarities

Contains 1 C-type lectin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 199199Early activation antigen CD69
PRO_0000046583

Regions

Topological domain1 – 4040Cytoplasmic Potential
Transmembrane41 – 6121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain62 – 199138Extracellular Potential
Domain92 – 195104C-type lectin

Amino acid modifications

Glycosylation1661N-linked (GlcNAc...) Potential
Disulfide bond68Interchain Probable
Disulfide bond85 ↔ 96 Ref.6 Ref.7 Ref.8
Disulfide bond113 ↔ 194 Ref.6 Ref.7 Ref.8
Disulfide bond173 ↔ 186 Ref.6 Ref.7 Ref.8

Secondary structure

...................... 199
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q07108 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 172E2699D2FB8DFB

FASTA19922,559
        10         20         30         40         50         60 
MSSENCFVAE NSSLHPESGQ ENDATSPHFS TRHEGSFQVP VLCAVMNVVF ITILIIALIA 

        70         80         90        100        110        120 
LSVGQYNCPG QYTFSMPSDS HVSSCSEDWV GYQRKCYFIS TVKRSWTSAQ NACSEHGATL 

       130        140        150        160        170        180 
AVIDSEKDMN FLKRYAGREE HWVGLKKEPG HPWKWSNGKE FNNWFNVTGS DKCVFLKNTE 

       190 
VSSMECEKNL YWICNKPYK 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain."
Hamann J., Fiebig H., Strauss M.
J. Immunol. 150:4920-4927(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Blood.
[2]"Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors."
Lopez-Cabrera M., Santis A.G., Fernandez-Ruiz E., Blacher R., Esch F., Sanchez-Mateos P., Sanchez-Madrid F.
J. Exp. Med. 178:537-547(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 96-103; 128-146 AND 189-199.
Tissue: Blood.
[3]"Molecular characterization of the early activation antigen CD69: a type II membrane glycoprotein related to a family of natural killer cell activation antigens."
Ziegler S.F., Ramsdell F., Hjerrild K.A., Armitage R.J., Grabstein K.H., Hennen K.B., Farrah T., Fanslow W.C., Shevach E.M., Alderson M.R.
Eur. J. Immunol. 23:1643-1648(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Structure of the gene coding for the human early lymphocyte activation antigen CD69: a C-type lectin receptor evolutionarily related with the gene families of natural killer cell-specific receptors."
Santis A., Lopez-Cabrera M., Hamann J., Strauss M., Sanchez-Madrid F.
Eur. J. Immunol. 24:1692-1697(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[6]"Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells."
Natarajan K., Sawicki M.W., Margulies D.H., Mariuzza R.A.
Biochemistry 39:14779-14786(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS), SUBUNIT, DISULFIDE BONDS.
[7]"Crystal structure of the C-type lectin-like domain from the human hematopoietic cell receptor CD69."
Llera A.S., Viedma F., Sanchez-Madrid F., Tormo J.
J. Biol. Chem. 276:7312-7319(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 82-199, SUBUNIT, DISULFIDE BONDS.
[8]"Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice."
Vanek O., Nalezkova M., Kavan D., Borovickova I., Pompach P., Novak P., Kumar V., Vannucci L., Hudecek J., Hofbauerova K., Kopecky V. Jr., Brynda J., Kolenko P., Dohnalek J., Kaderavek P., Chmelik J., Gorcik L., Zidek L., Sklenar V., Bezouska K.
FEBS J. 275:5589-5606(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 82-199, SUBUNIT, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07555 mRNA. Translation: AAB46359.1.
Z22576 mRNA. Translation: CAA80298.1.
Z30426 expand/collapse EMBL AC list , Z30430, Z30427, Z30429, Z30428 Genomic DNA. Translation: CAA83017.1.
BC007037 mRNA. Translation: AAH07037.1.
CCDSCCDS8604.1.
PIRJH0822.
RefSeqNP_001772.1. NM_001781.2.
UniGeneHs.208854.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E87X-ray1.50A82-199[»]
1E8IX-ray1.95A/B82-199[»]
1FM5X-ray2.27A1-199[»]
3CCKX-ray1.80A/B82-199[»]
3HUPX-ray1.37A/B70-199[»]
DisProtDP00306.
ProteinModelPortalQ07108.
SMRQ07108. Positions 79-199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60426N.
IntActQ07108. 1 interaction.
MINTMINT-4656238.
STRING9606.ENSP00000228434.

PTM databases

PhosphoSiteQ07108.

Polymorphism databases

DMDM584906.

Proteomic databases

MaxQBQ07108.
PaxDbQ07108.
PRIDEQ07108.

Protocols and materials databases

DNASU969.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000228434; ENSP00000228434; ENSG00000110848.
GeneID969.
KEGGhsa:969.
UCSCuc001qwk.3. human.

Organism-specific databases

CTD969.
GeneCardsGC12M010256.
HGNCHGNC:1694. CD69.
HPACAB002503.
MIM107273. gene.
neXtProtNX_Q07108.
PharmGKBPA26233.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG276386.
HOGENOMHOG000111491.
HOVERGENHBG005288.
InParanoidQ07108.
KOK06502.
OMAVGQYNCP.
OrthoDBEOG793B96.
PhylomeDBQ07108.
TreeFamTF351467.

Gene expression databases

ArrayExpressQ07108.
BgeeQ07108.
CleanExHS_CD69.
GenevestigatorQ07108.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ07108.
GeneWikiCD69.
GenomeRNAi969.
NextBio4056.
PROQ07108.
SOURCESearch...

Entry information

Entry nameCD69_HUMAN
AccessionPrimary (citable) accession number: Q07108
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries