Reviewed, UniProtKB/Swiss-Prot Q07108 (CD69_HUMAN)

Names and origin

Protein names

Recommended name:
    Early activation antigen CD69
Alternative name(s):
    Early T-cell activation antigen p60
    GP32/28
    Leu-23
    MLR-3
    EA1
    BL-AC/P26
    Activation inducer molecule
      Short name=AIM
    CD_antigen=CD69

Gene names

Name:

CD69

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
Developmental stage	Earliest inducible cell surface glycoprotein acquired during lymphoid activation.
Induction	By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.
Post-translational modification	Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	Lectin
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	sugar binding Inferred from electronic annotation. Source: InterPro transmembrane receptor activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 199

199

Early activation antigen CD69

PRO_0000046583

Regions

Topological domain

1 – 40

40

Cytoplasmic Potential

Transmembrane

41 – 61

21

Signal-anchor for type II membrane protein Potential

Topological domain

62 – 199

138

Extracellular Potential

Domain

92 – 195

104

C-type lectin

Amino acid modifications

Glycosylation

166

1

N-linked (GlcNAc...) Potential

Disulfide bond

113 ↔ 194

By similarity

Disulfide bond

173 ↔ 186

By similarity

Secondary structure

1

.

.

.

.

.

.

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.

.

.

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.

.

.

.

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199

Helix Strand Turn

Details...

Beta strand

89 – 92

4

Beta strand

95 – 99

5

Helix

106 – 115

10

Helix

126 – 136

11

Beta strand

141 – 146

6

Beta strand

168 – 170

3

Beta strand

172 – 177

6

Beta strand

180 – 184

5

Beta strand

190 – 197

8

Sequences

	Sequence		Length	Mass (Da)
	Q07108-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 172E2699D2FB8DFB	FASTA	199	22,559
	10 20 30 40 50 60 MSSENCFVAE NSSLHPESGQ ENDATSPHFS TRHEGSFQVP VLCAVMNVVF ITILIIALIA 70 80 90 100 110 120 LSVGQYNCPG QYTFSMPSDS HVSSCSEDWV GYQRKCYFIS TVKRSWTSAQ NACSEHGATL 130 140 150 160 170 180 AVIDSEKDMN FLKRYAGREE HWVGLKKEPG HPWKWSNGKE FNNWFNVTGS DKCVFLKNTE 190 VSSMECEKNL YWICNKPYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain." Hamann J., Fiebig H., Strauss M. J. Immunol. 150:4920-4927(1993) [PubMed: 8496594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
[2]	"Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors." Lopez-Cabrera M., Santis A.G., Fernandez-Ruiz E., Blacher R., Esch F., Sanchez-Mateos P., Sanchez-Madrid F. J. Exp. Med. 178:537-547(1993) [PubMed: 8340758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 96-103; 128-146 AND 189-199. Tissue: Blood.
[3]	"Molecular characterization of the early activation antigen CD69: a type II membrane glycoprotein related to a family of natural killer cell activation antigens." Ziegler S.F., Ramsdell F., Hjerrild K.A., Armitage R.J., Grabstein K.H., Hennen K.B., Farrah T., Fanslow W.C., Shevach E.M., Alderson M.R. Eur. J. Immunol. 23:1643-1648(1993) [PubMed: 8100776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Structure of the gene coding for the human early lymphocyte activation antigen CD69: a C-type lectin receptor evolutionarily related with the gene families of natural killer cell-specific receptors." Santis A., Lopez-Cabrera M., Hamann J., Strauss M., Sanchez-Madrid F. Eur. J. Immunol. 24:1692-1697(1994) [PubMed: 8026529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

CD69

Cross-references

Sequence databases

EMBLGenBankDDBJ

L07555 mRNA. Translation: AAB46359.1.
Z22576 mRNA. Translation: CAA80298.1.
Z30426 , Z30430, Z30427, Z30429, Z30428 Genomic DNA. Translation: CAA83017.1.
BC007037 mRNA. Translation: AAH07037.1.

PIR

JH0822.

RefSeq

NP_001772.1.

UniGene

Hs.208854

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E87	X-ray	1.50	A	82-199	[»]
1E8I	X-ray	1.95	A/B	82-199	[»]
1FM5	X-ray	2.27	A	1-199	[»]

DisProt

DP00306.

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000110848. Homo sapiens. [Contig view]

GeneID

969.

KEGG

hsa:969.

Organism-specific databases

H-InvDB

HIX0010418.

HGNC

HGNC:1694. CD69.

HPA

CAB002503.

MIM

107273. gene.

PharmGKB

PA26233.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q07108.

HOVERGEN

Q07108.

Gene expression databases

ArrayExpress

Q07108.

CleanEx

HS_CD69.

GermOnline

ENSG00000110848. Homo sapiens.

Family and domain databases

InterPro

IPR002353. AntifreezeII.
IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
[Graphical view]

Gene3D

G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.

Pfam

PF00059. Lectin_C. 1 hit.
[Graphical view]

PRINTS

PR00356. ANTIFREEZEII.

SMART

SM00034. CLECT. 1 hit.
[Graphical view]

PROSITE

PS00615. C_TYPE_LECTIN_1. False negative.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

4056.

SOURCE

Search...

Entry information

Entry name

CD69_HUMAN

Accession

Primary (citable) accession number: Q07108

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)