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Protein

Growth/differentiation factor 9

Gene

Gdf9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for ovarian folliculogenesis.

GO - Molecular functioni

  1. cytokine activity Source: GO_Central
  2. transforming growth factor beta receptor binding Source: GO_Central

GO - Biological processi

  1. BMP signaling pathway Source: GO_Central
  2. negative regulation of cell growth Source: MGI
  3. oocyte growth Source: MGI
  4. positive regulation of cell proliferation Source: MGI
  5. positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
  6. regulation of apoptotic process Source: GO_Central
  7. regulation of MAPK cascade Source: GO_Central
  8. regulation of progesterone secretion Source: MGI
  9. SMAD protein signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 9
Short name:
GDF-9
Gene namesi
Name:Gdf9
Synonyms:Gdf-9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:95692. Gdf9.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Propeptidei30 – 306277Sequence AnalysisPRO_0000033982Add
BLAST
Chaini307 – 441135Growth/differentiation factor 9PRO_0000033983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi340 ↔ 406By similarity
Disulfide bondi369 ↔ 438By similarity
Disulfide bondi373 ↔ 440By similarity

Post-translational modificationi

Phosphorylated; phosphorylation is critical for GDF9 function.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ07105.

PTM databases

PhosphoSiteiQ07105.

Expressioni

Tissue specificityi

Ovary.

Gene expression databases

BgeeiQ07105.
CleanExiMM_GDF9.
ExpressionAtlasiQ07105. baseline.
GenevestigatoriQ07105.

Interactioni

Subunit structurei

Homodimer or heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked.Curated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018382.

Structurei

3D structure databases

ProteinModelPortaliQ07105.
SMRiQ07105. Positions 340-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG261730.
HOGENOMiHOG000073526.
HOVERGENiHBG004660.
InParanoidiQ07105.
KOiK05498.
OMAiDNWIVAP.
OrthoDBiEOG70W3DJ.
PhylomeDBiQ07105.
TreeFamiTF351788.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR015617. Growth_differentiation_fac-9.
IPR001839. TGF-b_C.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PTHR11848:SF19. PTHR11848:SF19. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPSNFLLG VCCFAWLCFL SSLSSQASTE ESQSGASENV ESEADPWSLL
60 70 80 90 100
LPVDGTDRSG LLPPLFKVLS DRRGETPKLQ PDSRALYYMK KLYKTYATKE
110 120 130 140 150
GVPKPSRSHL YNTVRLFSPC AQQEQAPSNQ VTGPLPMVDL LFNLDRVTAM
160 170 180 190 200
EHLLKSVLLY TLNNSASSSS TVTCMCDLVV KEAMSSGRAP PRAPYSFTLK
210 220 230 240 250
KHRWIEIDVT SLLQPLVTSS ERSIHLSVNF TCTKDQVPED GVFSMPLSVP
260 270 280 290 300
PSLILYLNDT STQAYHSWQS LQSTWRPLQH PGQAGVAARP VKEEAIEVER
310 320 330 340 350
SPRRRRGQKA IRSEAKGPLL TASFNLSEYF KQFLFPQNEC ELHDFRLSFS
360 370 380 390 400
QLKWDNWIVA PHRYNPRYCK GDCPRAVRHR YGSPVHTMVQ NIIYEKLDPS
410 420 430 440
VPRPSCVPGK YSPLSVLTIE PDGSIAYKEY EDMIATRCTC R
Length:441
Mass (Da):49,649
Last modified:November 23, 2004 - v2
Checksum:i43B47ADD41B99C29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti296 – 2961I → T in AAA53035 (PubMed:8429021).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06444 mRNA. Translation: AAA53035.1.
X77112 Genomic DNA. No translation available.
X77113 Genomic DNA. No translation available.
CCDSiCCDS24679.1.
PIRiS45284.
RefSeqiNP_032136.2. NM_008110.2.
UniGeneiMm.490461.
Mm.9714.

Genome annotation databases

EnsembliENSMUST00000018382; ENSMUSP00000018382; ENSMUSG00000018238.
GeneIDi14566.
KEGGimmu:14566.
UCSCiuc007iwa.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06444 mRNA. Translation: AAA53035.1.
X77112 Genomic DNA. No translation available.
X77113 Genomic DNA. No translation available.
CCDSiCCDS24679.1.
PIRiS45284.
RefSeqiNP_032136.2. NM_008110.2.
UniGeneiMm.490461.
Mm.9714.

3D structure databases

ProteinModelPortaliQ07105.
SMRiQ07105. Positions 340-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018382.

PTM databases

PhosphoSiteiQ07105.

Proteomic databases

PRIDEiQ07105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018382; ENSMUSP00000018382; ENSMUSG00000018238.
GeneIDi14566.
KEGGimmu:14566.
UCSCiuc007iwa.2. mouse.

Organism-specific databases

CTDi2661.
MGIiMGI:95692. Gdf9.

Phylogenomic databases

eggNOGiNOG261730.
HOGENOMiHOG000073526.
HOVERGENiHBG004660.
InParanoidiQ07105.
KOiK05498.
OMAiDNWIVAP.
OrthoDBiEOG70W3DJ.
PhylomeDBiQ07105.
TreeFamiTF351788.

Miscellaneous databases

ChiTaRSiGdf9. mouse.
NextBioi286274.
PROiQ07105.
SOURCEiSearch...

Gene expression databases

BgeeiQ07105.
CleanExiMM_GDF9.
ExpressionAtlasiQ07105. baseline.
GenevestigatoriQ07105.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR015617. Growth_differentiation_fac-9.
IPR001839. TGF-b_C.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PTHR11848:SF19. PTHR11848:SF19. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "GDF-3 and GDF-9: two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines."
    McPherron A.C., Lee S.-J.
    J. Biol. Chem. 268:3444-3449(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the mouse growth/differentiation factor 9 gene."
    Incerti B., Dong J., Borsani G., Matzuk M.M.
    Biochim. Biophys. Acta 1222:125-128(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvEv.
  3. "The ratio of growth differentiation factor 9: bone morphogenetic protein 15 mRNA expression is tightly co-regulated and differs between species over a wide range of ovulation rates."
    Crawford J.L., McNatty K.P.
    Mol. Cell. Endocrinol. 348:339-343(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SPECIES-SPECIFIC OVULATION RATE DETERMINATION.

Entry informationi

Entry nameiGDF9_MOUSE
AccessioniPrimary (citable) accession number: Q07105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Ovarian physiology and fertility are controlled by endocrine and paracrine signals. These act in a species-dependent manner and determine the ovulation quota in different mammalian species. While humans, and mammals such as the cow or red deer, normally ovulate only one egg per cycle, other mammals such as mouse and pig can ovulate in excess of ten per cycle. The mechanisms that regulate the species-specific differences in the number of follicles that go onto ovulate during each reproductive cycle are poorly understood. According to PubMed:21970812, mRNA expression levels of GDF9 and BMP15 are tightly coregulated within each species and influence species-specific ovulation-rates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.