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Q07100 (PP2A4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP2A-4 catalytic subunit

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2A isoform 4
Gene names
Name:PP2A4
Synonyms:PP2A3
Ordered Locus Names:At2g42500
ORF Names:MHK10.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Sequence caution

The sequence AAD10854.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q07100-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Serine/threonine-protein phosphatase PP2A-4 catalytic subunit
PRO_0000058854

Sites

Active site1221Proton donor By similarity
Metal binding611Iron By similarity
Metal binding631Iron By similarity
Metal binding891Iron By similarity
Metal binding891Manganese By similarity
Metal binding1211Manganese By similarity
Metal binding1711Manganese By similarity
Metal binding2451Manganese By similarity

Experimental info

Sequence conflict151D → N in AAD10854. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 2EF8542AD8A89C64

FASTA31335,833
        10         20         30         40         50         60 
MGANSIPTDA TIDLDEQISQ LMQCKPLSEQ QVRALCEKAK EILMDESNVQ PVKSPVTICG 

        70         80         90        100        110        120 
DIHGQFHDLA ELFRIGGMCP DTNYLFMGDY VDRGYYSVET VTLLVALKMR YPQRITILRG 

       130        140        150        160        170        180 
NHESRQITQV YGFYDECLRK YGNANVWKIF TDLFDYFPLT ALVESEIFCL HGGLSPSIET 

       190        200        210        220        230        240 
LDNIRNFDRV QEVPHEGPMC DLLWSDPDDR CGWGISPRGA GYTFGQDISE QFNHTNNLKL 

       250        260        270        280        290        300 
IARAHQLVMD GYNWAHEQKV VTIFSAPNYC YRCGNMASIL EVDDCRNHTF IQFEPAPRRG 

       310 
EPDVTRRTPD YFL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of a fourth isoform of the catalytic subunit of protein phosphatase 2A from Arabidopsis thaliana."
Casamayor A., Perez-Callejon E., Pujol G., Arino J., Ferrer A.
Plant Mol. Biol. 26:523-528(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia GL1.
Tissue: Leaf.
[2]"Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana."
Perez-Callejon E., Casamayor A., Pujol G., Camps M., Ferrer A., Arino J.
Gene 209:105-112(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana."
Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A.
Plant Mol. Biol. 21:475-485(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-313.
Strain: cv. Columbia GL1.
[7]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96841 mRNA. Translation: AAA64742.1.
U60135 Genomic DNA. Translation: AAD10854.1. Sequence problems.
AC005956 Genomic DNA. Translation: AAD23731.1.
AC007087 Genomic DNA. Translation: AAM15383.1.
CP002685 Genomic DNA. Translation: AEC10127.1.
BT010166 mRNA. Translation: AAQ22635.1.
PIRS31163.
S52659.
RefSeqNP_565974.1. NM_129811.3.
UniGeneAt.20031.

3D structure databases

ProteinModelPortalQ07100.
SMRQ07100. Positions 11-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4187. 3 interactions.

Proteomic databases

PaxDbQ07100.
PRIDEQ07100.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G42500.1; AT2G42500.1; AT2G42500. [Q07100-1]
GeneID818850.
KEGGath:AT2G42500.

Organism-specific databases

TAIRAT2G42500.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
InParanoidQ07100.
KOK04382.
OMAEVDEQHN.
PhylomeDBQ07100.
ProtClustDBCLSN2688889.

Enzyme and pathway databases

BioCycARA:AT2G42500-MONOMER.
ARA:GQT-402-MONOMER.

Gene expression databases

GenevestigatorQ07100.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2A4_ARATH
AccessionPrimary (citable) accession number: Q07100
Secondary accession number(s): Q7G9R3, Q9ZRF6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names