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Protein

Serine/threonine-protein phosphatase PP2A-4 catalytic subunit

Gene

PP2A4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Manganese 1By similarity
Metal bindingi63 – 631Manganese 1By similarity
Metal bindingi89 – 891Manganese 1By similarity
Metal bindingi89 – 891Manganese 2By similarity
Metal bindingi121 – 1211Manganese 2By similarity
Active sitei122 – 1221Proton donorBy similarity
Metal bindingi171 – 1711Manganese 2By similarity
Metal bindingi245 – 2451Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G42500-MONOMER.
ARA:GQT-402-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-4 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 4
Gene namesi
Name:PP2A4
Synonyms:PP2A3
Ordered Locus Names:At2g42500
ORF Names:MHK10.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G42500.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Serine/threonine-protein phosphatase PP2A-4 catalytic subunitPRO_0000058854Add
BLAST

Proteomic databases

PaxDbiQ07100.
PRIDEiQ07100.

Expressioni

Gene expression databases

ExpressionAtlasiQ07100. baseline.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families).By similarity

Protein-protein interaction databases

BioGridi4187. 3 interactions.
STRINGi3702.AT2G42500.1.

Structurei

3D structure databases

ProteinModelPortaliQ07100.
SMRiQ07100. Positions 11-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ07100.
OMAiETHRINP.
PhylomeDBiQ07100.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q07100-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGANSIPTDA TIDLDEQISQ LMQCKPLSEQ QVRALCEKAK EILMDESNVQ
60 70 80 90 100
PVKSPVTICG DIHGQFHDLA ELFRIGGMCP DTNYLFMGDY VDRGYYSVET
110 120 130 140 150
VTLLVALKMR YPQRITILRG NHESRQITQV YGFYDECLRK YGNANVWKIF
160 170 180 190 200
TDLFDYFPLT ALVESEIFCL HGGLSPSIET LDNIRNFDRV QEVPHEGPMC
210 220 230 240 250
DLLWSDPDDR CGWGISPRGA GYTFGQDISE QFNHTNNLKL IARAHQLVMD
260 270 280 290 300
GYNWAHEQKV VTIFSAPNYC YRCGNMASIL EVDDCRNHTF IQFEPAPRRG
310
EPDVTRRTPD YFL
Length:313
Mass (Da):35,833
Last modified:February 1, 1996 - v2
Checksum:i2EF8542AD8A89C64
GO

Sequence cautioni

The sequence AAD10854.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151D → N in AAD10854 (PubMed:9524239).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96841 mRNA. Translation: AAA64742.1.
U60135 Genomic DNA. Translation: AAD10854.1. Sequence problems.
AC005956 Genomic DNA. Translation: AAD23731.1.
AC007087 Genomic DNA. Translation: AAM15383.1.
CP002685 Genomic DNA. Translation: AEC10127.1.
BT010166 mRNA. Translation: AAQ22635.1.
PIRiS31163.
S52659.
RefSeqiNP_565974.1. NM_129811.3. [Q07100-1]
UniGeneiAt.20031.

Genome annotation databases

EnsemblPlantsiAT2G42500.1; AT2G42500.1; AT2G42500. [Q07100-1]
GeneIDi818850.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96841 mRNA. Translation: AAA64742.1.
U60135 Genomic DNA. Translation: AAD10854.1. Sequence problems.
AC005956 Genomic DNA. Translation: AAD23731.1.
AC007087 Genomic DNA. Translation: AAM15383.1.
CP002685 Genomic DNA. Translation: AEC10127.1.
BT010166 mRNA. Translation: AAQ22635.1.
PIRiS31163.
S52659.
RefSeqiNP_565974.1. NM_129811.3. [Q07100-1]
UniGeneiAt.20031.

3D structure databases

ProteinModelPortaliQ07100.
SMRiQ07100. Positions 11-313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4187. 3 interactions.
STRINGi3702.AT2G42500.1.

Proteomic databases

PaxDbiQ07100.
PRIDEiQ07100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42500.1; AT2G42500.1; AT2G42500. [Q07100-1]
GeneIDi818850.

Organism-specific databases

TAIRiAT2G42500.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ07100.
OMAiETHRINP.
PhylomeDBiQ07100.

Enzyme and pathway databases

BioCyciARA:AT2G42500-MONOMER.
ARA:GQT-402-MONOMER.

Miscellaneous databases

PROiQ07100.

Gene expression databases

ExpressionAtlasiQ07100. baseline.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a fourth isoform of the catalytic subunit of protein phosphatase 2A from Arabidopsis thaliana."
    Casamayor A., Perez-Callejon E., Pujol G., Arino J., Ferrer A.
    Plant Mol. Biol. 26:523-528(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia GL1.
    Tissue: Leaf.
  2. "Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana."
    Perez-Callejon E., Casamayor A., Pujol G., Camps M., Ferrer A., Arino J.
    Gene 209:105-112(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana."
    Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A.
    Plant Mol. Biol. 21:475-485(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-313.
    Strain: cv. Columbia GL1.
  7. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPP2A4_ARATH
AccessioniPrimary (citable) accession number: Q07100
Secondary accession number(s): Q7G9R3, Q9ZRF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.