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Q07099 (PP2A1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2A isoform 1
Gene names
Name:PP2A1
Synonyms:PP2A2
Ordered Locus Names:At1g59830
ORF Names:F23H11.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) By similarity. Interacts with TAF12B. Ref.6

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q07099-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q07099-2)

The sequence of this isoform differs from the canonical sequence as follows:
     250-262: EKNVVTVFSAPNY → VYNMCITIDWHMC
     263-306: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 306306Serine/threonine-protein phosphatase PP2A-1 catalytic subunit
PRO_0000058853

Sites

Active site1151Proton donor By similarity
Metal binding541Manganese 1 By similarity
Metal binding561Manganese 1 By similarity
Metal binding821Manganese 1 By similarity
Metal binding821Manganese 2 By similarity
Metal binding1141Manganese 2 By similarity
Metal binding1641Manganese 2 By similarity
Metal binding2381Manganese 2 By similarity

Natural variations

Alternative sequence250 – 26213EKNVV…SAPNY → VYNMCITIDWHMC in isoform 2.
VSP_028727
Alternative sequence263 – 30644Missing in isoform 2.
VSP_028728

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 579427029D41A1C1

FASTA30634,961
        10         20         30         40         50         60 
MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT VCGDIHGQFY 

        70         80         90        100        110        120 
DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL KVRYRDRLTI LRGNHESRQI 

       130        140        150        160        170        180 
TQVYGFYDEC LRKYGNANVW KYFTDLFDYL PLTALIESQV FCLHGGLSPS LDTLDNIRSL 

       190        200        210        220        230        240 
DRIQEVPHEG PMCDLLWSDP DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL 

       250        260        270        280        290        300 
VMEGYNWCQE KNVVTVFSAP NYCYRCGNMA AILEIGEKME QNFLQFDPAP RQVEPDTTRK 


TPDYFL 

« Hide

Isoform 2 [UniParc].

Checksum: 5731D406F8E63A9B
Show »

FASTA26230,002

References

« Hide 'large scale' references
[1]"Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana."
Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A.
Plant Mol. Biol. 21:475-485(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Columbia GL1.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
J. Exp. Bot. 58:2627-2639(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAF12B.
Strain: cv. Wassilewskija.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96732 mRNA. Translation: AAA32847.1.
AC007258 Genomic DNA. Translation: AAD39326.1.
CP002684 Genomic DNA. Translation: AEE33622.1.
CP002684 Genomic DNA. Translation: AEE33623.1.
AY063942 mRNA. Translation: AAL36298.1.
AY096543 mRNA. Translation: AAM20193.1.
PIRS31161.
RefSeqNP_176192.1. NM_104676.4. [Q07099-1]
NP_974050.1. NM_202321.2. [Q07099-2]
UniGeneAt.315.

3D structure databases

ProteinModelPortalQ07099.
SMRQ07099. Positions 6-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27501. 2 interactions.

Proteomic databases

PaxDbQ07099.
PRIDEQ07099.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G59830.1; AT1G59830.1; AT1G59830. [Q07099-1]
GeneID842276.
KEGGath:AT1G59830.

Organism-specific databases

TAIRAT1G59830.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
InParanoidQ07099.
KOK04382.
OMARPPDYFL.
PhylomeDBQ07099.

Enzyme and pathway databases

BioCycARA:AT1G59830-MONOMER.
ARA:GQT-2736-MONOMER.

Gene expression databases

GenevestigatorQ07099.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2A1_ARATH
AccessionPrimary (citable) accession number: Q07099
Secondary accession number(s): Q3ECM1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names