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Protein

Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

Gene

PP2A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Manganese 1By similarity1
Metal bindingi56Manganese 1By similarity1
Metal bindingi82Manganese 1By similarity1
Metal bindingi82Manganese 2By similarity1
Metal bindingi114Manganese 2By similarity1
Active sitei115Proton donorBy similarity1
Metal bindingi164Manganese 2By similarity1
Metal bindingi238Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 1
Gene namesi
Name:PP2A11 Publication
Synonyms:PP2A2
Ordered Locus Names:At1g59830
ORF Names:F23H11.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G59830
TAIRilocus:2025976 AT1G59830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588531 – 306Serine/threonine-protein phosphatase PP2A-1 catalytic subunitAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphotyrosineBy similarity1
Modified residuei306Leucine methyl ester1 Publication1

Post-translational modificationi

Reversibly methyl esterified on Leu-306 by leucine carboxyl methyltransferase 1 (LCMT1) and pectin methylesterase 1 (PME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.1 Publication
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ07099
PRIDEiQ07099

Expressioni

Gene expression databases

ExpressionAtlasiQ07099 baseline and differential
GenevisibleiQ07099 AT

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity). Interacts with TAF12B (By similarity) (PubMed:17526916). Interacts with SRK2E/OST1 (PubMed:26175513). Interacts with TAP46.By similarity3 Publications

Protein-protein interaction databases

BioGridi27501, 3 interactors
STRINGi3702.AT1G59830.1

Structurei

3D structure databases

ProteinModelPortaliQ07099
SMRiQ07099
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0371 Eukaryota
COG0639 LUCA
HOGENOMiHOG000172696
InParanoidiQ07099
KOiK04382
OMAiMECKPLG
OrthoDBiEOG09360EXD
PhylomeDBiQ07099

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q07099-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT
60 70 80 90 100
VCGDIHGQFY DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL
110 120 130 140 150
KVRYRDRLTI LRGNHESRQI TQVYGFYDEC LRKYGNANVW KYFTDLFDYL
160 170 180 190 200
PLTALIESQV FCLHGGLSPS LDTLDNIRSL DRIQEVPHEG PMCDLLWSDP
210 220 230 240 250
DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL VMEGYNWCQE
260 270 280 290 300
KNVVTVFSAP NYCYRCGNMA AILEIGEKME QNFLQFDPAP RQVEPDTTRK

TPDYFL
Length:306
Mass (Da):34,961
Last modified:October 1, 1994 - v1
Checksum:i579427029D41A1C1
GO
Isoform 2 (identifier: Q07099-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-262: EKNVVTVFSAPNY → VYNMCITIDWHMC
     263-306: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:262
Mass (Da):30,002
Checksum:i5731D406F8E63A9B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028727250 – 262EKNVV…SAPNY → VYNMCITIDWHMC in isoform 2. CuratedAdd BLAST13
Alternative sequenceiVSP_028728263 – 306Missing in isoform 2. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96732 mRNA Translation: AAA32847.1
AC007258 Genomic DNA Translation: AAD39326.1
CP002684 Genomic DNA Translation: AEE33622.1
CP002684 Genomic DNA Translation: AEE33623.1
AY063942 mRNA Translation: AAL36298.1
AY096543 mRNA Translation: AAM20193.1
PIRiS31161
RefSeqiNP_176192.1, NM_104676.5 [Q07099-1]
NP_974050.1, NM_202321.2 [Q07099-2]
UniGeneiAt.315

Genome annotation databases

EnsemblPlantsiAT1G59830.1; AT1G59830.1; AT1G59830 [Q07099-1]
AT1G59830.2; AT1G59830.2; AT1G59830 [Q07099-2]
GeneIDi842276
GrameneiAT1G59830.1; AT1G59830.1; AT1G59830 [Q07099-1]
AT1G59830.2; AT1G59830.2; AT1G59830 [Q07099-2]
KEGGiath:AT1G59830

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiPP2A1_ARATH
AccessioniPrimary (citable) accession number: Q07099
Secondary accession number(s): Q3ECM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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