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Q07099

- PP2A1_ARATH

UniProt

Q07099 - PP2A1_ARATH

Protein

Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

Gene

PP2A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi56 – 561Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G59830-MONOMER.
    ARA:GQT-2736-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2A isoform 1
    Gene namesi
    Name:PP2A1
    Synonyms:PP2A2
    Ordered Locus Names:At1g59830
    ORF Names:F23H11.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G59830.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. nucleus Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306Serine/threonine-protein phosphatase PP2A-1 catalytic subunitPRO_0000058853Add
    BLAST

    Proteomic databases

    PaxDbiQ07099.
    PRIDEiQ07099.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07099.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) By similarity. Interacts with TAF12B.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi27501. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07099.
    SMRiQ07099. Positions 6-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    InParanoidiQ07099.
    KOiK04382.
    OMAiRPPDYFL.
    PhylomeDBiQ07099.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q07099-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT    50
    VCGDIHGQFY DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL 100
    KVRYRDRLTI LRGNHESRQI TQVYGFYDEC LRKYGNANVW KYFTDLFDYL 150
    PLTALIESQV FCLHGGLSPS LDTLDNIRSL DRIQEVPHEG PMCDLLWSDP 200
    DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL VMEGYNWCQE 250
    KNVVTVFSAP NYCYRCGNMA AILEIGEKME QNFLQFDPAP RQVEPDTTRK 300
    TPDYFL 306
    Length:306
    Mass (Da):34,961
    Last modified:October 1, 1994 - v1
    Checksum:i579427029D41A1C1
    GO
    Isoform 2 (identifier: Q07099-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         250-262: EKNVVTVFSAPNY → VYNMCITIDWHMC
         263-306: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:262
    Mass (Da):30,002
    Checksum:i5731D406F8E63A9B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei250 – 26213EKNVV…SAPNY → VYNMCITIDWHMC in isoform 2. CuratedVSP_028727Add
    BLAST
    Alternative sequencei263 – 30644Missing in isoform 2. CuratedVSP_028728Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96732 mRNA. Translation: AAA32847.1.
    AC007258 Genomic DNA. Translation: AAD39326.1.
    CP002684 Genomic DNA. Translation: AEE33622.1.
    CP002684 Genomic DNA. Translation: AEE33623.1.
    AY063942 mRNA. Translation: AAL36298.1.
    AY096543 mRNA. Translation: AAM20193.1.
    PIRiS31161.
    RefSeqiNP_176192.1. NM_104676.4. [Q07099-1]
    NP_974050.1. NM_202321.2. [Q07099-2]
    UniGeneiAt.315.

    Genome annotation databases

    EnsemblPlantsiAT1G59830.1; AT1G59830.1; AT1G59830. [Q07099-1]
    GeneIDi842276.
    KEGGiath:AT1G59830.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96732 mRNA. Translation: AAA32847.1 .
    AC007258 Genomic DNA. Translation: AAD39326.1 .
    CP002684 Genomic DNA. Translation: AEE33622.1 .
    CP002684 Genomic DNA. Translation: AEE33623.1 .
    AY063942 mRNA. Translation: AAL36298.1 .
    AY096543 mRNA. Translation: AAM20193.1 .
    PIRi S31161.
    RefSeqi NP_176192.1. NM_104676.4. [Q07099-1 ]
    NP_974050.1. NM_202321.2. [Q07099-2 ]
    UniGenei At.315.

    3D structure databases

    ProteinModelPortali Q07099.
    SMRi Q07099. Positions 6-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27501. 2 interactions.

    Proteomic databases

    PaxDbi Q07099.
    PRIDEi Q07099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G59830.1 ; AT1G59830.1 ; AT1G59830 . [Q07099-1 ]
    GeneIDi 842276.
    KEGGi ath:AT1G59830.

    Organism-specific databases

    TAIRi AT1G59830.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    InParanoidi Q07099.
    KOi K04382.
    OMAi RPPDYFL.
    PhylomeDBi Q07099.

    Enzyme and pathway databases

    BioCyci ARA:AT1G59830-MONOMER.
    ARA:GQT-2736-MONOMER.

    Gene expression databases

    Genevestigatori Q07099.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana."
      Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A.
      Plant Mol. Biol. 21:475-485(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Columbia GL1.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
      Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
      J. Exp. Bot. 58:2627-2639(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAF12B.
      Strain: cv. Wassilewskija.

    Entry informationi

    Entry nameiPP2A1_ARATH
    AccessioniPrimary (citable) accession number: Q07099
    Secondary accession number(s): Q3ECM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3