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Protein

Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

Gene

PP2A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Manganese 1By similarity
Metal bindingi56 – 561Manganese 1By similarity
Metal bindingi82 – 821Manganese 1By similarity
Metal bindingi82 – 821Manganese 2By similarity
Metal bindingi114 – 1141Manganese 2By similarity
Active sitei115 – 1151Proton donorBy similarity
Metal bindingi164 – 1641Manganese 2By similarity
Metal bindingi238 – 2381Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G59830-MONOMER.
ARA:GQT-2736-MONOMER.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_286793. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_293115. Glycolysis.
REACT_310958. ERKs are inactivated.
REACT_318739. Integration of energy metabolism.
REACT_319752. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 1
Gene namesi
Name:PP2A1
Synonyms:PP2A2
Ordered Locus Names:At1g59830
ORF Names:F23H11.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G59830.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. nucleus Source: TAIR
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Serine/threonine-protein phosphatase PP2A-1 catalytic subunitPRO_0000058853Add
BLAST

Proteomic databases

PaxDbiQ07099.
PRIDEiQ07099.

Expressioni

Gene expression databases

ExpressionAtlasiQ07099. baseline and differential.
GenevestigatoriQ07099.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity). Interacts with TAF12B.By similarity1 Publication

Protein-protein interaction databases

BioGridi27501. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ07099.
SMRiQ07099. Positions 6-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ07099.
KOiK04382.
OMAiRALAYCL.
PhylomeDBiQ07099.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q07099-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT
60 70 80 90 100
VCGDIHGQFY DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL
110 120 130 140 150
KVRYRDRLTI LRGNHESRQI TQVYGFYDEC LRKYGNANVW KYFTDLFDYL
160 170 180 190 200
PLTALIESQV FCLHGGLSPS LDTLDNIRSL DRIQEVPHEG PMCDLLWSDP
210 220 230 240 250
DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL VMEGYNWCQE
260 270 280 290 300
KNVVTVFSAP NYCYRCGNMA AILEIGEKME QNFLQFDPAP RQVEPDTTRK

TPDYFL
Length:306
Mass (Da):34,961
Last modified:September 30, 1994 - v1
Checksum:i579427029D41A1C1
GO
Isoform 2 (identifier: Q07099-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-262: EKNVVTVFSAPNY → VYNMCITIDWHMC
     263-306: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:262
Mass (Da):30,002
Checksum:i5731D406F8E63A9B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei250 – 26213EKNVV…SAPNY → VYNMCITIDWHMC in isoform 2. CuratedVSP_028727Add
BLAST
Alternative sequencei263 – 30644Missing in isoform 2. CuratedVSP_028728Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96732 mRNA. Translation: AAA32847.1.
AC007258 Genomic DNA. Translation: AAD39326.1.
CP002684 Genomic DNA. Translation: AEE33622.1.
CP002684 Genomic DNA. Translation: AEE33623.1.
AY063942 mRNA. Translation: AAL36298.1.
AY096543 mRNA. Translation: AAM20193.1.
PIRiS31161.
RefSeqiNP_176192.1. NM_104676.4. [Q07099-1]
NP_974050.1. NM_202321.2. [Q07099-2]
UniGeneiAt.315.

Genome annotation databases

EnsemblPlantsiAT1G59830.1; AT1G59830.1; AT1G59830. [Q07099-1]
GeneIDi842276.
KEGGiath:AT1G59830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96732 mRNA. Translation: AAA32847.1.
AC007258 Genomic DNA. Translation: AAD39326.1.
CP002684 Genomic DNA. Translation: AEE33622.1.
CP002684 Genomic DNA. Translation: AEE33623.1.
AY063942 mRNA. Translation: AAL36298.1.
AY096543 mRNA. Translation: AAM20193.1.
PIRiS31161.
RefSeqiNP_176192.1. NM_104676.4. [Q07099-1]
NP_974050.1. NM_202321.2. [Q07099-2]
UniGeneiAt.315.

3D structure databases

ProteinModelPortaliQ07099.
SMRiQ07099. Positions 6-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27501. 2 interactions.

Proteomic databases

PaxDbiQ07099.
PRIDEiQ07099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G59830.1; AT1G59830.1; AT1G59830. [Q07099-1]
GeneIDi842276.
KEGGiath:AT1G59830.

Organism-specific databases

TAIRiAT1G59830.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ07099.
KOiK04382.
OMAiRALAYCL.
PhylomeDBiQ07099.

Enzyme and pathway databases

BioCyciARA:AT1G59830-MONOMER.
ARA:GQT-2736-MONOMER.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_286793. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_293115. Glycolysis.
REACT_310958. ERKs are inactivated.
REACT_318739. Integration of energy metabolism.
REACT_319752. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Gene expression databases

ExpressionAtlasiQ07099. baseline and differential.
GenevestigatoriQ07099.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana."
    Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A.
    Plant Mol. Biol. 21:475-485(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia GL1.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
    Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
    J. Exp. Bot. 58:2627-2639(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAF12B.
    Strain: cv. Wassilewskija.

Entry informationi

Entry nameiPP2A1_ARATH
AccessioniPrimary (citable) accession number: Q07099
Secondary accession number(s): Q3ECM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1994
Last sequence update: September 30, 1994
Last modified: March 31, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.