Q07099 (PP2A1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2A isoform 1

Gene names

Name:	PP2A1
Synonyms:	PP2A2
Ordered Locus Names:	At1g59830
ORF Names:	F23H11.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 22404109. Source: TAIR nucleus Inferred from direct assay PubMed 22404109. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q07099-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q07099-2)

The sequence of this isoform differs from the canonical sequence as follows:
     250-262: EKNVVTVFSAPNY → VYNMCITIDWHMC
     263-306: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Serine/threonine-protein phosphatase PP2A-1 catalytic subunit		PRO_0000058853
Sites
	Active site	115	1	Proton donor By similarity
	Metal binding	54	1	Iron By similarity
	Metal binding	56	1	Iron By similarity
	Metal binding	82	1	Iron By similarity
	Metal binding	82	1	Manganese By similarity
	Metal binding	114	1	Manganese By similarity
	Metal binding	164	1	Manganese By similarity
	Metal binding	238	1	Manganese By similarity
Natural variations
	Alternative sequence	250 – 262	13	EKNVV…SAPNY → VYNMCITIDWHMC in isoform 2.		VSP_028727
	Alternative sequence	263 – 306	44	Missing in isoform 2.		VSP_028728

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 579427029D41A1C1	FASTA	306	34,961
	10 20 30 40 50 60 MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT VCGDIHGQFY 70 80 90 100 110 120 DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL KVRYRDRLTI LRGNHESRQI 130 140 150 160 170 180 TQVYGFYDEC LRKYGNANVW KYFTDLFDYL PLTALIESQV FCLHGGLSPS LDTLDNIRSL 190 200 210 220 230 240 DRIQEVPHEG PMCDLLWSDP DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL 250 260 270 280 290 300 VMEGYNWCQE KNVVTVFSAP NYCYRCGNMA AILEIGEKME QNFLQFDPAP RQVEPDTTRK TPDYFL
	Isoform 2 [UniParc]. Checksum: 5731D406F8E63A9B	FASTA	262	30,002
	10 20 30 40 50 60 MPLNGDLDRQ IEQLMECKPL GEADVKILCD QAKAILVEEY NVQPVKCPVT VCGDIHGQFY 70 80 90 100 110 120 DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL KVRYRDRLTI LRGNHESRQI 130 140 150 160 170 180 TQVYGFYDEC LRKYGNANVW KYFTDLFDYL PLTALIESQV FCLHGGLSPS LDTLDNIRSL 190 200 210 220 230 240 DRIQEVPHEG PMCDLLWSDP DDRCGWGISP RGAGYTFGQD IATQFNHNNG LSLISRAHQL 250 260 VMEGYNWCQV YNMCITIDWH MC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana." Arino J., Perez-Callejon E., Cunillera N., Camps M., Posas F., Ferrer A. Plant Mol. Biol. 21:475-485(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Columbia GL1.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96732 mRNA. Translation: AAA32847.1. AC007258 Genomic DNA. Translation: AAD39326.1. CP002684 Genomic DNA. Translation: AEE33622.1. CP002684 Genomic DNA. Translation: AEE33623.1. AY063942 mRNA. Translation: AAL36298.1. AY096543 mRNA. Translation: AAM20193.1.
IPI	IPI00533336. IPI00541192.
PIR	S31161.
RefSeq	NP_176192.1. NM_104676.4. NP_974050.1. NM_202321.2.
UniGene	At.315.
3D structure databases
ProteinModelPortal	Q07099.
SMR	Q07099. Positions 6-290.
ModBase	Search...
Proteomic databases
PaxDb	Q07099.
PRIDE	Q07099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G59830.1; AT1G59830.1; AT1G59830.
GeneID	842276.
KEGG	ath:AT1G59830.
Organism-specific databases
TAIR	At1g59830.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172696.
InParanoid	Q07099.
KO	K04382.
OMA	TFNHANR.
PhylomeDB	Q07099.
ProtClustDB	CLSN2679580.
Gene expression databases
ArrayExpress	Q07099.
Genevestigator	Q07099.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2A1_ARATH

Accession

Primary (citable) accession number: Q07099
Secondary accession number(s): Q3ECM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program