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Protein

Serine/threonine-protein phosphatase PP2A-2 catalytic subunit

Gene

PP2A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dephosphorylates and activates the actin-depolymerizing factor ADF1, which, in turn, regulates actin cytoskeleton remodeling and is involved in the blue light photoreceptor PHOT2-mediated chloroplast avoidance movements (PubMed:22642987). Associates with the serine/threonine-protein phosphatase PP2A regulatory subunits A and B' to positively regulates beta-oxidation of fatty acids and protoauxins in peroxisomes by dephosphorylating peroxisomal beta-oxidation-related proteins (PubMed:25489022). Acts as negative regulator of abscisic acid (ABA) signaling. May regulate ABA-dependent gene expression (PubMed:17617176). Involved in the light-dependent activation of nitrate reductase (PubMed:28656346).4 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Manganese 1By similarity1
Metal bindingi56Manganese 1By similarity1
Metal bindingi82Manganese 1By similarity1
Metal bindingi82Manganese 2By similarity1
Metal bindingi114Manganese 2By similarity1
Active sitei115Proton donorBy similarity1
Metal bindingi164Manganese 2By similarity1
Metal bindingi238Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • cellular protein localization Source: TAIR
  • chloroplast avoidance movement Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • positive regulation of fatty acid beta-oxidation Source: UniProtKB
  • protein dephosphorylation Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processAbscisic acid signaling pathway
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-2 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 2
Gene namesi
Name:PP2A21 Publication
Synonyms:PP2A1
Ordered Locus Names:At1g10430
ORF Names:T10O24.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G10430
TAIRilocus:2194626 AT1G10430

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588521 – 306Serine/threonine-protein phosphatase PP2A-2 catalytic subunitAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphotyrosineBy similarity1
Modified residuei306Leucine methyl ester1 Publication1

Post-translational modificationi

Reversibly methyl esterified on Leu-306 by leucine carboxyl methyltransferase 1 (LCMT1) and pectin methylesterase 1 (PME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.1 Publication
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ07098
PRIDEiQ07098

Expressioni

Tissue specificityi

Expressed in root meristem, emerging lateral roots, leaf vasculature, stipules, guard cells, anthers and pollen grains.1 Publication

Inductioni

Induced by abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ07098 baseline and differential
GenevisibleiQ07098 AT

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (Probable). Interacts with B'THETA (PubMed:25489022). Interacts with HDA14 (PubMed:22404109). Interacts with SRK2E/OST1 (PubMed:26175513). Interacts with TAP46.1 Publication4 Publications

Protein-protein interaction databases

BioGridi22823, 4 interactors
IntActiQ07098, 1 interactor
STRINGi3702.AT1G10430.1

Structurei

3D structure databases

ProteinModelPortaliQ07098
SMRiQ07098
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0371 Eukaryota
COG0639 LUCA
HOGENOMiHOG000172696
InParanoidiQ07098
KOiK04382
OMAiHESKQIT
OrthoDBiEOG09360EXD
PhylomeDBiQ07098

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q07098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSNGDLDRQ IEQLMECKPL SEADVRTLCD QARAILVEEY NVQPVKCPVT
60 70 80 90 100
VCGDIHGQFY DLIELFRIGG NAPDTNYLFM GDYVDRGYYS VETVSLLVAL
110 120 130 140 150
KVRYRDRLTI LRGNHESRQI TQVYGFYDEC LRKYGNANVW KYFTDLFDYL
160 170 180 190 200
PLTALIESQV FCLHGGLSPS LDTLDNIRSL DRIQEVPHEG PMCDLLWSDP
210 220 230 240 250
DDRCGWGISP RGAGYTFGQD IAAQFNHNNG LSLISRAHQL VMEGFNWCQD
260 270 280 290 300
KNVVTVFSAP NYCYRCGNMA AILEIGENME QNFLQFDPAP RQVEPDTTRK

TPDYFL
Length:306
Mass (Da):34,934
Last modified:October 1, 1994 - v1
Checksum:iB2DCB1B3C2CDD54F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96733 mRNA Translation: AAA32848.1
AC007067 Genomic DNA Translation: AAD39564.1
CP002684 Genomic DNA Translation: AEE28579.1
CP002684 Genomic DNA Translation: ANM59883.1
AY059847 mRNA Translation: AAL24329.1
AY093267 mRNA Translation: AAM13266.1
PIRiS31162
RefSeqiNP_001322206.1, NM_001331905.1
NP_172514.1, NM_100918.3
UniGeneiAt.21842

Genome annotation databases

EnsemblPlantsiAT1G10430.1; AT1G10430.1; AT1G10430
AT1G10430.2; AT1G10430.2; AT1G10430
GeneIDi837583
GrameneiAT1G10430.1; AT1G10430.1; AT1G10430
AT1G10430.2; AT1G10430.2; AT1G10430
KEGGiath:AT1G10430

Similar proteinsi

Entry informationi

Entry nameiPP2A2_ARATH
AccessioniPrimary (citable) accession number: Q07098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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