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Protein

Glucokinase regulatory protein

Gene

Gckr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531Fructose 1-phosphateBy similarity
Binding sitei348 – 3481Fructose 1-phosphateBy similarity
Binding sitei514 – 5141Fructose 1-phosphate/Fructose 6-phosphate

GO - Molecular functioni

  • carbohydrate binding Source: RGD
  • enzyme binding Source: RGD
  • enzyme inhibitor activity Source: RGD
  • fructose-6-phosphate binding Source: UniProtKB
  • protein domain specific binding Source: RGD

GO - Biological processi

  • cellular glucose homeostasis Source: Ensembl
  • detection of glucose Source: BHF-UCL
  • glucose metabolic process Source: RGD
  • negative regulation of catalytic activity Source: RGD
  • negative regulation of glucokinase activity Source: BHF-UCL
  • positive regulation of glucokinase activity Source: Ensembl
  • protein import into nucleus, translocation Source: BHF-UCL
  • response to fructose Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKQ07071.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase regulatory protein
Short name:
Glucokinase regulator
Gene namesi
Name:Gckr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2671. Gckr.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • mitochondrion Source: RGD
  • nucleoplasm Source: UniProtKB
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3124733.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Glucokinase regulatory proteinPRO_0000214827Add
BLAST

Proteomic databases

PaxDbiQ07071.
PRIDEiQ07071.

PTM databases

iPTMnetiQ07071.
PhosphoSiteiQ07071.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Detected in liver. Not detected in muscle, brain, heart, testis, intestine or spleen.2 Publications

Gene expression databases

BgeeiENSRNOG00000048874.
GenevisibleiQ07071. RN.

Interactioni

Subunit structurei

Interacts (fructose 6-phosphate bound form) with GCK.2 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ07071. 3 interactions.
MINTiMINT-1795091.
STRINGi10116.ENSRNOP00000064260.

Chemistry

BindingDBiQ07071.

Structurei

Secondary structure

1
627
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni23 – 253Combined sources
Turni30 – 323Combined sources
Turni39 – 435Combined sources
Helixi46 – 5813Combined sources
Helixi59 – 613Combined sources
Beta strandi66 – 683Combined sources
Helixi77 – 9519Combined sources
Beta strandi100 – 1078Combined sources
Helixi108 – 12720Combined sources
Beta strandi134 – 1385Combined sources
Helixi143 – 1464Combined sources
Helixi150 – 1545Combined sources
Helixi156 – 16712Combined sources
Beta strandi171 – 1788Combined sources
Helixi185 – 19511Combined sources
Turni198 – 2003Combined sources
Beta strandi201 – 2066Combined sources
Helixi211 – 2133Combined sources
Helixi226 – 23712Combined sources
Turni238 – 2403Combined sources
Beta strandi243 – 2453Combined sources
Turni258 – 2603Combined sources
Helixi261 – 28020Combined sources
Helixi289 – 30719Combined sources
Helixi310 – 32516Combined sources
Beta strandi330 – 3356Combined sources
Helixi337 – 35216Combined sources
Turni357 – 3593Combined sources
Beta strandi360 – 3656Combined sources
Helixi397 – 3993Combined sources
Beta strandi405 – 4117Combined sources
Helixi416 – 42510Combined sources
Beta strandi433 – 4408Combined sources
Helixi447 – 4526Combined sources
Beta strandi457 – 4615Combined sources
Turni467 – 4693Combined sources
Helixi470 – 49526Combined sources
Turni496 – 4983Combined sources
Helixi513 – 52715Combined sources
Helixi531 – 54313Combined sources
Helixi550 – 5534Combined sources
Helixi557 – 5648Combined sources
Helixi570 – 57910Combined sources
Helixi584 – 5929Combined sources
Helixi597 – 6048Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LC9X-ray3.40A1-627[»]
ProteinModelPortaliQ07071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 286197SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini320 – 499180SIS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 1102Fructose 1-phosphate/Fructose 6-phosphate binding
Regioni179 – 1813Fructose 1-phosphate/Fructose 6-phosphate binding
Regioni199 – 2002Important for interaction with GCK
Regioni463 – 4653Essential for interaction with GCK

Domaini

Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.

Sequence similaritiesi

Belongs to the GCKR family.Curated
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOVERGENiHBG005818.
InParanoidiQ07071.
OMAiAGQMDYC.
OrthoDBiEOG091G03ET.
PhylomeDBiQ07071.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTKRYQHV IETPEPGEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV
60 70 80 90 100
KLLGQCDAEI FQEEGQIVPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG
110 120 130 140 150
LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE
160 170 180 190 200
QTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV
210 220 230 240 250
FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG
260 270 280 290 300
PEGLSGSSRM KGGGATKILL ETLLLAAHKT VDQGVVSSQR CLLEILRTFE
310 320 330 340 350
RAHQVTYSQS SKIATLMKQV GISLEKKGRV HLVGWQTLGI IAIMDGVECI
360 370 380 390 400
HTFGADFQDI RGFLIGDHSD MFNQKDELTN QGPQFTFSQD DFLTSILPSL
410 420 430 440 450
TETDTVVFIF TLDDNLTEVQ ALAERVREKC QNIQALVHST VGQSLPAPLK
460 470 480 490 500
KLFPSLISIT WPLLFFDYEG TYVQKFQREL STKWVLNTVS TGAHVLLGKI
510 520 530 540 550
LQNHMLDLRI ANSKLFWRAL AMLQRFSGQS KARCIESLLQ AIHFPQPLSD
560 570 580 590 600
DVRAAPISCH VQVAHEKEKV IPTALLSLLL RCSISEAKAR LSAASSVCEV
610 620
VRSALSGPGQ KRSTQALEDP PACGTLN
Length:627
Mass (Da):68,917
Last modified:January 23, 2007 - v3
Checksum:i2C330146A0289928
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68497 mRNA. Translation: CAA48511.1.
BC081843 mRNA. Translation: AAH81843.1.
PIRiS41745.
RefSeqiNP_037252.1. NM_013120.2.
UniGeneiRn.7863.

Genome annotation databases

EnsembliENSRNOT00000073228; ENSRNOP00000064260; ENSRNOG00000048874.
GeneIDi25658.
KEGGirno:25658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68497 mRNA. Translation: CAA48511.1.
BC081843 mRNA. Translation: AAH81843.1.
PIRiS41745.
RefSeqiNP_037252.1. NM_013120.2.
UniGeneiRn.7863.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LC9X-ray3.40A1-627[»]
ProteinModelPortaliQ07071.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ07071. 3 interactions.
MINTiMINT-1795091.
STRINGi10116.ENSRNOP00000064260.

Chemistry

BindingDBiQ07071.
ChEMBLiCHEMBL3124733.

PTM databases

iPTMnetiQ07071.
PhosphoSiteiQ07071.

Proteomic databases

PaxDbiQ07071.
PRIDEiQ07071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073228; ENSRNOP00000064260; ENSRNOG00000048874.
GeneIDi25658.
KEGGirno:25658.

Organism-specific databases

CTDi2646.
RGDi2671. Gckr.

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOVERGENiHBG005818.
InParanoidiQ07071.
OMAiAGQMDYC.
OrthoDBiEOG091G03ET.
PhylomeDBiQ07071.

Enzyme and pathway databases

ReactomeiR-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKQ07071.

Miscellaneous databases

PROiQ07071.

Gene expression databases

BgeeiENSRNOG00000048874.
GenevisibleiQ07071. RN.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCKR_RAT
AccessioniPrimary (citable) accession number: Q07071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.