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Protein

Glucokinase regulatory protein

Gene

Gckr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei153Fructose 1-phosphateBy similarity1
Binding sitei348Fructose 1-phosphateBy similarity1
Binding sitei514Fructose 1-phosphate/Fructose 6-phosphate1

GO - Molecular functioni

  • carbohydrate binding Source: RGD
  • enzyme binding Source: RGD
  • enzyme inhibitor activity Source: RGD
  • fructose-6-phosphate binding Source: UniProtKB
  • protein domain specific binding Source: RGD

GO - Biological processi

  • cellular glucose homeostasis Source: Ensembl
  • detection of glucose Source: BHF-UCL
  • glucose metabolic process Source: RGD
  • negative regulation of catalytic activity Source: RGD
  • negative regulation of glucokinase activity Source: BHF-UCL
  • positive regulation of glucokinase activity Source: Ensembl
  • protein import into nucleus, translocation Source: BHF-UCL
  • response to fructose Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKQ07071.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase regulatory protein
Short name:
Glucokinase regulator
Gene namesi
Name:Gckr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2671. Gckr.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • mitochondrion Source: RGD
  • nucleoplasm Source: UniProtKB
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3124733.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002148271 – 627Glucokinase regulatory proteinAdd BLAST627

Proteomic databases

PaxDbiQ07071.
PRIDEiQ07071.

PTM databases

iPTMnetiQ07071.
PhosphoSitePlusiQ07071.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Detected in liver. Not detected in muscle, brain, heart, testis, intestine or spleen.2 Publications

Gene expression databases

BgeeiENSRNOG00000048874.
GenevisibleiQ07071. RN.

Interactioni

Subunit structurei

Interacts (fructose 6-phosphate bound form) with GCK.2 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ07071. 3 interactors.
MINTiMINT-1795091.
STRINGi10116.ENSRNOP00000064260.

Chemistry databases

BindingDBiQ07071.

Structurei

Secondary structure

1627
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni23 – 25Combined sources3
Turni30 – 32Combined sources3
Turni39 – 43Combined sources5
Helixi46 – 58Combined sources13
Helixi59 – 61Combined sources3
Beta strandi66 – 68Combined sources3
Helixi77 – 95Combined sources19
Beta strandi100 – 107Combined sources8
Helixi108 – 127Combined sources20
Beta strandi134 – 138Combined sources5
Helixi143 – 146Combined sources4
Helixi150 – 154Combined sources5
Helixi156 – 167Combined sources12
Beta strandi171 – 178Combined sources8
Helixi185 – 195Combined sources11
Turni198 – 200Combined sources3
Beta strandi201 – 206Combined sources6
Helixi211 – 213Combined sources3
Helixi226 – 237Combined sources12
Turni238 – 240Combined sources3
Beta strandi243 – 245Combined sources3
Turni258 – 260Combined sources3
Helixi261 – 280Combined sources20
Helixi289 – 307Combined sources19
Helixi310 – 325Combined sources16
Beta strandi330 – 335Combined sources6
Helixi337 – 352Combined sources16
Turni357 – 359Combined sources3
Beta strandi360 – 365Combined sources6
Helixi397 – 399Combined sources3
Beta strandi405 – 411Combined sources7
Helixi416 – 425Combined sources10
Beta strandi433 – 440Combined sources8
Helixi447 – 452Combined sources6
Beta strandi457 – 461Combined sources5
Turni467 – 469Combined sources3
Helixi470 – 495Combined sources26
Turni496 – 498Combined sources3
Helixi513 – 527Combined sources15
Helixi531 – 543Combined sources13
Helixi550 – 553Combined sources4
Helixi557 – 564Combined sources8
Helixi570 – 579Combined sources10
Helixi584 – 592Combined sources9
Helixi597 – 604Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LC9X-ray3.40A1-627[»]
ProteinModelPortaliQ07071.
SMRiQ07071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 286SIS 1PROSITE-ProRule annotationAdd BLAST197
Domaini320 – 499SIS 2PROSITE-ProRule annotationAdd BLAST180

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 110Fructose 1-phosphate/Fructose 6-phosphate binding2
Regioni179 – 181Fructose 1-phosphate/Fructose 6-phosphate binding3
Regioni199 – 200Important for interaction with GCK1 Publication2
Regioni463 – 465Essential for interaction with GCK1 Publication3

Domaini

Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.

Sequence similaritiesi

Belongs to the GCKR family.Curated
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOVERGENiHBG005818.
InParanoidiQ07071.
OMAiAGQMDYC.
OrthoDBiEOG091G03ET.
PhylomeDBiQ07071.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTKRYQHV IETPEPGEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV
60 70 80 90 100
KLLGQCDAEI FQEEGQIVPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG
110 120 130 140 150
LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE
160 170 180 190 200
QTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV
210 220 230 240 250
FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG
260 270 280 290 300
PEGLSGSSRM KGGGATKILL ETLLLAAHKT VDQGVVSSQR CLLEILRTFE
310 320 330 340 350
RAHQVTYSQS SKIATLMKQV GISLEKKGRV HLVGWQTLGI IAIMDGVECI
360 370 380 390 400
HTFGADFQDI RGFLIGDHSD MFNQKDELTN QGPQFTFSQD DFLTSILPSL
410 420 430 440 450
TETDTVVFIF TLDDNLTEVQ ALAERVREKC QNIQALVHST VGQSLPAPLK
460 470 480 490 500
KLFPSLISIT WPLLFFDYEG TYVQKFQREL STKWVLNTVS TGAHVLLGKI
510 520 530 540 550
LQNHMLDLRI ANSKLFWRAL AMLQRFSGQS KARCIESLLQ AIHFPQPLSD
560 570 580 590 600
DVRAAPISCH VQVAHEKEKV IPTALLSLLL RCSISEAKAR LSAASSVCEV
610 620
VRSALSGPGQ KRSTQALEDP PACGTLN
Length:627
Mass (Da):68,917
Last modified:January 23, 2007 - v3
Checksum:i2C330146A0289928
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68497 mRNA. Translation: CAA48511.1.
BC081843 mRNA. Translation: AAH81843.1.
PIRiS41745.
RefSeqiNP_037252.1. NM_013120.2.
UniGeneiRn.7863.

Genome annotation databases

EnsembliENSRNOT00000073228; ENSRNOP00000064260; ENSRNOG00000048874.
GeneIDi25658.
KEGGirno:25658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68497 mRNA. Translation: CAA48511.1.
BC081843 mRNA. Translation: AAH81843.1.
PIRiS41745.
RefSeqiNP_037252.1. NM_013120.2.
UniGeneiRn.7863.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LC9X-ray3.40A1-627[»]
ProteinModelPortaliQ07071.
SMRiQ07071.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ07071. 3 interactors.
MINTiMINT-1795091.
STRINGi10116.ENSRNOP00000064260.

Chemistry databases

BindingDBiQ07071.
ChEMBLiCHEMBL3124733.

PTM databases

iPTMnetiQ07071.
PhosphoSitePlusiQ07071.

Proteomic databases

PaxDbiQ07071.
PRIDEiQ07071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073228; ENSRNOP00000064260; ENSRNOG00000048874.
GeneIDi25658.
KEGGirno:25658.

Organism-specific databases

CTDi2646.
RGDi2671. Gckr.

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOVERGENiHBG005818.
InParanoidiQ07071.
OMAiAGQMDYC.
OrthoDBiEOG091G03ET.
PhylomeDBiQ07071.

Enzyme and pathway databases

ReactomeiR-RNO-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKQ07071.

Miscellaneous databases

PROiQ07071.

Gene expression databases

BgeeiENSRNOG00000048874.
GenevisibleiQ07071. RN.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCKR_RAT
AccessioniPrimary (citable) accession number: Q07071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.