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Protein

Cytoskeleton-associated protein 4

Gene

CKAP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity epithelial cell surface receptor for APF.
Mediates the anchoring of the endoplasmic reticulum to microtubules.

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton-associated protein 4
Alternative name(s):
63-kDa cytoskeleton-linking membrane protein
Short name:
Climp-63
Short name:
p63
Gene namesi
Name:CKAP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:16991. CKAP4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 106106CytoplasmicSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Topological domaini128 – 602475ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • lamellar body Source: Reactome
  • lipid particle Source: UniProtKB
  • membrane Source: ProtInc
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26527.

Polymorphism and mutation databases

BioMutaiCKAP4.
DMDMi74735614.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Cytoskeleton-associated protein 4PRO_0000252417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphoserine1 Publication
Modified residuei17 – 171PhosphoserineCombined sources1 Publication
Modified residuei19 – 191Phosphoserine1 Publication
Modified residuei21 – 211N6-acetyllysineBy similarity
Lipidationi100 – 1001S-palmitoyl cysteine; by ZDHHC21 Publication
Modified residuei232 – 2321Phosphoserine; by FAM20C1 Publication
Modified residuei312 – 3121PhosphoserineCombined sources

Post-translational modificationi

Reversibly palmitoylated. Palmitoylation at Cys-100 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization.2 Publications
Increased phosphorylation during mitosis prevents binding to microtubules.1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ07065.
MaxQBiQ07065.
PaxDbiQ07065.
PRIDEiQ07065.

PTM databases

iPTMnetiQ07065.
PhosphoSiteiQ07065.
SwissPalmiQ07065.

Expressioni

Gene expression databases

BgeeiQ07065.
CleanExiHS_CKAP4.
ExpressionAtlasiQ07065. baseline and differential.
GenevisibleiQ07065. HS.

Organism-specific databases

HPAiHPA000278.
HPA000792.
HPA001225.

Interactioni

Protein-protein interaction databases

BioGridi116167. 94 interactions.
IntActiQ07065. 46 interactions.
MINTiMINT-1413290.
STRINGi9606.ENSP00000367265.

Structurei

3D structure databases

ProteinModelPortaliQ07065.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili130 – 21485Sequence analysisAdd
BLAST
Coiled coili256 – 460205Sequence analysisAdd
BLAST
Coiled coili533 – 60270Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi36 – 5924Pro-richAdd
BLAST
Compositional biasi71 – 777Poly-Gly
Compositional biasi85 – 9410Poly-Ala
Compositional biasi118 – 1214Poly-Ala

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIRS. Eukaryota.
ENOG4111Q54. LUCA.
GeneTreeiENSGT00390000015968.
HOGENOMiHOG000060146.
HOVERGENiHBG101940.
InParanoidiQ07065.
KOiK13999.
OMAiSEGYKQD.
OrthoDBiEOG7VX8VQ.
PhylomeDBiQ07065.
TreeFamiTF332395.

Family and domain databases

InterProiIPR028428. Ckap4.
[Graphical view]
PANTHERiPTHR18937:SF243. PTHR18937:SF243. 3 hits.

Sequencei

Sequence statusi: Complete.

Q07065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSAKQRGSK GGHGAASPSE KGAHPSGGAD DVAKKPPPAP QQPPPPPAPH
60 70 80 90 100
PQQHPQQHPQ NQAHGKGGHR GGGGGGGKSS SSSSASAAAA AAAASSSASC
110 120 130 140 150
SRRLGRALNF LFYLALVAAA AFSGWCVHHV LEEVQQVRRS HQDFSRQREE
160 170 180 190 200
LGQGLQGVEQ KVQSLQATFG TFESILRSSQ HKQDLTEKAV KQGESEVSRI
210 220 230 240 250
SEVLQKLQNE ILKDLSDGIH VVKDARERDF TSLENTVEER LTELTKSIND
260 270 280 290 300
NIAIFTEVQK RSQKEINDMK AKVASLEESE GNKQDLKALK EAVKEIQTSA
310 320 330 340 350
KSREWDMEAL RSTLQTMESD IYTEVRELVS LKQEQQAFKE AADTERLALQ
360 370 380 390 400
ALTEKLLRSE ESVSRLPEEI RRLEEELRQL KSDSHGPKED GGFRHSEAFE
410 420 430 440 450
ALQQKSQGLD SRLQHVEDGV LSMQVASARQ TESLESLLSK SQEHEQRLAA
460 470 480 490 500
LQGRLEGLGS SEADQDGLAS TVRSLGETQL VLYGDVEELK RSVGELPSTV
510 520 530 540 550
ESLQKVQEQV HTLLSQDQAQ AARLPPQDFL DRLSSLDNLK ASVSQVEADL
560 570 580 590 600
KMLRTAVDSL VAYSVKIETN ENNLESAKGL LDDLRNDLDR LFVKVEKIHE

KV
Length:602
Mass (Da):66,022
Last modified:June 1, 2002 - v2
Checksum:i216E6A92ED5E058A
GO

Sequence cautioni

The sequence AAH94824.1 differs from that shown.Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601Q → R in BAD97283 (Ref. 2) Curated
Sequence conflicti362 – 3621S → P in AAH94824 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti348 – 3481A → T.
Corresponds to variant rs3088113 [ dbSNP | Ensembl ].
VAR_027853

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69910 mRNA. Translation: CAA49535.2.
AK223563 mRNA. Translation: BAD97283.1.
AC079174 Genomic DNA. No translation available.
BC082972 mRNA. Translation: AAH82972.1.
BC094824 mRNA. Translation: AAH94824.1. Sequence problems.
CCDSiCCDS9103.1.
PIRiS33377.
RefSeqiNP_006816.2. NM_006825.3.
UniGeneiHs.74368.

Genome annotation databases

EnsembliENST00000378026; ENSP00000367265; ENSG00000136026.
GeneIDi10970.
KEGGihsa:10970.
UCSCiuc001tlk.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69910 mRNA. Translation: CAA49535.2.
AK223563 mRNA. Translation: BAD97283.1.
AC079174 Genomic DNA. No translation available.
BC082972 mRNA. Translation: AAH82972.1.
BC094824 mRNA. Translation: AAH94824.1. Sequence problems.
CCDSiCCDS9103.1.
PIRiS33377.
RefSeqiNP_006816.2. NM_006825.3.
UniGeneiHs.74368.

3D structure databases

ProteinModelPortaliQ07065.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116167. 94 interactions.
IntActiQ07065. 46 interactions.
MINTiMINT-1413290.
STRINGi9606.ENSP00000367265.

PTM databases

iPTMnetiQ07065.
PhosphoSiteiQ07065.
SwissPalmiQ07065.

Polymorphism and mutation databases

BioMutaiCKAP4.
DMDMi74735614.

Proteomic databases

EPDiQ07065.
MaxQBiQ07065.
PaxDbiQ07065.
PRIDEiQ07065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378026; ENSP00000367265; ENSG00000136026.
GeneIDi10970.
KEGGihsa:10970.
UCSCiuc001tlk.4. human.

Organism-specific databases

CTDi10970.
GeneCardsiCKAP4.
HGNCiHGNC:16991. CKAP4.
HPAiHPA000278.
HPA000792.
HPA001225.
neXtProtiNX_Q07065.
PharmGKBiPA26527.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIRS. Eukaryota.
ENOG4111Q54. LUCA.
GeneTreeiENSGT00390000015968.
HOGENOMiHOG000060146.
HOVERGENiHBG101940.
InParanoidiQ07065.
KOiK13999.
OMAiSEGYKQD.
OrthoDBiEOG7VX8VQ.
PhylomeDBiQ07065.
TreeFamiTF332395.

Enzyme and pathway databases

ReactomeiR-HSA-5683826. Surfactant metabolism.

Miscellaneous databases

ChiTaRSiCKAP4. human.
GeneWikiiCKAP4.
GenomeRNAii10970.
NextBioi41682.
PROiQ07065.

Gene expression databases

BgeeiQ07065.
CleanExiHS_CKAP4.
ExpressionAtlasiQ07065. baseline and differential.
GenevisibleiQ07065. HS.

Family and domain databases

InterProiIPR028428. Ckap4.
[Graphical view]
PANTHERiPTHR18937:SF243. PTHR18937:SF243. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A reversibly palmitoylated resident protein(p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein."
    Schweizer A., Rohrer J., Jenoe P., De Maio A., Buchman T.G., Hauri H.-P.
    J. Cell Sci. 104:685-694(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 312-326; 413-429 AND 491-504, SUBCELLULAR LOCATION, PALMITOYLATION.
    Tissue: Placenta.
  2. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Pancreas.
  5. "Phosphorylation controls CLIMP-63-mediated anchoring of the endoplasmic reticulum to microtubules."
    Vedrenne C., Klopfenstein D.R., Hauri H.P.
    Mol. Biol. Cell 16:1928-1937(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CYTOSKELETON ANCHORING, PHOSPHORYLATION AT SER-3; SER-17 AND SER-19.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "CKAP4/p63 is a receptor for the frizzled-8 protein-related antiproliferative factor from interstitial cystitis patients."
    Conrads T.P., Tocci G.M., Hood B.L., Zhang C.O., Guo L., Koch K.R., Michejda C.J., Veenstra T.D., Keay S.K.
    J. Biol. Chem. 281:37836-37843(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS APF RECEPTOR, SUBCELLULAR LOCATION.
  8. "Climp-63-mediated binding of microtubules to the ER affects the lateral mobility of translocon complexes."
    Nikonov A.V., Hauri H.P., Lauring B., Kreibich G.
    J. Cell Sci. 120:2248-2258(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CYTOSKELETON ANCHORING, SUBCELLULAR LOCATION.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Palmitoylation of cytoskeleton associated protein 4 by DHHC2 regulates antiproliferative factor-mediated signaling."
    Planey S.L., Keay S.K., Zhang C.O., Zacharias D.A.
    Mol. Biol. Cell 20:1454-1463(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PALMITOYLATION AT CYS-100 BY ZDHHC2.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  14. Cited for: PHOSPHORYLATION AT SER-232.
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCKAP4_HUMAN
AccessioniPrimary (citable) accession number: Q07065
Secondary accession number(s): Q504S5, Q53ES6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2002
Last modified: April 13, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.