ID   FTHS_CLOCY              Reviewed;         556 AA.
AC   Q07064;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   08-NOV-2023, entry version 86.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
OS   Clostridium cylindrosporum.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1495;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8443597; DOI=10.1002/pro.5560020208;
RA   Rankin C.A., Haslam G.C., Himes R.H.;
RT   "Sequence and expression of the gene for N10-formyltetrahydrofolate
RT   synthetase from Clostridium cylindrosporum.";
RL   Protein Sci. 2:197-205(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; L12465; AAA23239.1; -; Genomic_DNA.
DR   PIR; I40808; I40808.
DR   AlphaFoldDB; Q07064; -.
DR   SMR; Q07064; -.
DR   BioCyc; MetaCyc:MONOMER-13114; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..556
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199339"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   556 AA;  59096 MW;  44C16B0A957890FB CRC64;
     MKTDVQIAQE AQMKPITEVA NYLGIQDDEL ELYGKYKAKV SLDVLERQKD KEDAKLVLVT
     AINPTPAGEG KTTTNVGLSM GLNKIGKRTI TALREPSLGP CFGVKGGAAG GGYAQVVPMD
     DINLHFTGDF HAITSAHNLL AALLDNHLHQ GNALNINPKK IVWKRVIDMN DRSLRNVIIG
     LGGNGDGFVR QAQFDITVAS EIMAILCLAT SMSDLKERLS KMIVAYAKDG SAVTAGQLEA
     TGAMALLLKD AVKPNLVQTL ENTPAFIHGG PFANIAHGCN SVLATKVALK LADYVVTEGG
     FGADLGAEKF FDIKSRFAGL KPNCDVSVAT VRALKMNGGV PKTELAAENV EAVKKGVANL
     ERHIENVAKF GVPAVVAINK FPLDTEAELK AVEDACNAKG ADVVLSDVWA NGGEGGVEMA
     KKVVEICEKN EANFAPLYDV NLSIPEKIEK IATTIYRADG VDFTSDCKKQ IAELEKLGLD
     KMPICMAKTQ YSFSDDPTLL GAPTGFRITV REVRVSAGAG FIVALTGNMM TMPGLPKVPA
     ANGMDILESG EIIGLS
//