Reviewed, UniProtKB/Swiss-Prot Q07024 (RFBA_VIBCH)

Names and origin

Protein names

Recommended name:
    Putative mannose-1-phosphate guanylyltransferase
    EC=2.7.7.13
Alternative name(s):
    GDP-mannose pyrophosphorylase
      Short name=GMPP
      Short name=GMP

Gene names

Name:	rfbA
Ordered Locus Names:	VC_0241

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.
Sequence caution	The sequence CAA42134.1 differs from that shown. Reason: Frameshift at position 14.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Putative mannose-1-phosphate guanylyltransferase		PRO_0000194262

Sequences

	Sequence		Length	Mass (Da)
	Q07024-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7274A5A876F49268	FASTA	465	51,916
	10 20 30 40 50 60 MFIPVIMAGG SGSRLWPLSR SAFPKQFLSL DSSSQHTMLQ ATIERLQGLP IAEPIVISNE 70 80 90 100 110 120 DHRFIVAEQI RRYGKKSRII LEPAGRNTAP AIALAAFTAI EQEDDPVLLV LAADHFVKNK 130 140 150 160 170 180 SAFQAAISQA AQQAEAGKLA TFGIVPTTPE TGYGYIHRGE EVTQGTYEIN SFVEKPQLNI 190 200 210 220 230 240 AEQYLASGEY YWNSGCFMFK ASVFLNELKQ HSPEIYRQCE LAMQGLSHDY DFIRVGVEEF 250 260 270 280 290 300 LKCPDDSIDY AVMEHTKLGV VVSMDAGWSD VGSWSALWEV SDKDADGNVC QGDAILSGTS 310 320 330 340 350 360 NCYIYAPNKL VAAVGLKDIV VVETKDAVLV ADKNQVQEVK KIVEHLKAEN RAEYREHRER 370 380 390 400 410 420 YRPWGKSDAI DKGERYKVNR ITVEPGKKQS LQMHYHRAEH WVVVSGTAKV TCEGNVKVIT 430 440 450 460 ENQSLYIPIG TNHMIENPGK IPLELIEIQS GSYLNEDDVV RFEDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Serotype conversion in Vibrio cholerae O1." Stroeher U.H., Karageorgos L.E., Morona R., Manning P.A. Proc. Natl. Acad. Sci. U.S.A. 89:2566-2570(1992) [PubMed: 1372980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor O17 / Serotype O1.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59554 Genomic DNA. Translation: CAA42134.1. Frameshift. AE003852 Genomic DNA. Translation: AAF93417.1.
PIR	H82345. S28468.
RefSeq	NP_229898.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2614704.
GenomeReviews	Gene locus VC_0241 in contig AE003852_GR.
KEGG	vch:VC0241.
TIGR	VC_0241.
Phylogenomic databases
HOGENOM	Q07024.
OMA	ASESIDY.
Enzyme and pathway databases
BRENDA	2.7.7.22. 19019.
Family and domain databases
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
ProDom	PD002664. Man6P_isomerII. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFBA_VIBCH

Accession

Primary (citable) accession number: Q07024
Secondary accession number(s): Q9KVA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)