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Q07010 (HPRT_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine-guanine phosphoribosyltransferase

Short name=HGPRT
Short name=HGPRTase
EC=2.4.2.8
Gene names
Name:HGPRT
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactor

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139596

Regions

Nucleotide binding113 – 1219GMP By similarity

Sites

Active site1171Proton acceptor By similarity
Metal binding1731Magnesium By similarity
Binding site541GMP By similarity
Binding site1451GMP By similarity
Binding site1731GMP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q07010 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 6134FA880F40EC3D

FASTA21023,360
        10         20         30         40         50         60 
MEPACKYDFA TSVLFTEAEL HTRMRGVAQR IADDYSNCNL KPLENPLVIV SVLKGSFVFT 

        70         80         90        100        110        120 
ADMVRILGDF GVPTRVEFLR ASSYGHDTKS CGRVDVKADG LCDIRGKHVL VLEDILDTAL 

       130        140        150        160        170        180 
TLREVVDSLK KSEPASIKTL VAIDKPGGRK IPFTAEYVVA DVPNVFVVGY GLDYDQSYRE 

       190        200        210 
VRDVVILKPS VYETWGKELE RRKAAGEAKR 

« Hide

References

[1]"Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma brucei."
Allen T.E., Ullman B.
Nucleic Acids Res. 21:5431-5438(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10721 Unassigned DNA. Translation: AAA16328.1.
PIRS41631.
RefSeqXP_822398.1. XM_817305.1.

3D structure databases

ProteinModelPortalQ07010.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ07010.
ChEMBLCHEMBL5069.

Proteomic databases

PRIDEQ07010.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3662982.
KEGGtbr:Tb10.70.6650.

Phylogenomic databases

KOK00760.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15863.
UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
InterProIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01203. HGPRTase. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_TRYBB
AccessionPrimary (citable) accession number: Q07010
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways