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Q07010

- HPRT_TRYBB

UniProt

Q07010 - HPRT_TRYBB

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Protein
Hypoxanthine-guanine phosphoribosyltransferase
Gene
HGPRT
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541GMP By similarity
Active sitei117 – 1171Proton acceptor By similarity
Binding sitei145 – 1451GMP By similarity
Metal bindingi173 – 1731Magnesium By similarity
Binding sitei173 – 1731GMP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi113 – 1219GMP By similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15863.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HGPRT
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139596Add
BLAST

Proteomic databases

PRIDEiQ07010.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ07010.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00760.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07010-1 [UniParc]FASTAAdd to Basket

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MEPACKYDFA TSVLFTEAEL HTRMRGVAQR IADDYSNCNL KPLENPLVIV    50
SVLKGSFVFT ADMVRILGDF GVPTRVEFLR ASSYGHDTKS CGRVDVKADG 100
LCDIRGKHVL VLEDILDTAL TLREVVDSLK KSEPASIKTL VAIDKPGGRK 150
IPFTAEYVVA DVPNVFVVGY GLDYDQSYRE VRDVVILKPS VYETWGKELE 200
RRKAAGEAKR 210
Length:210
Mass (Da):23,360
Last modified:June 1, 1994 - v1
Checksum:i6134FA880F40EC3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10721 Unassigned DNA. Translation: AAA16328.1.
PIRiS41631.

Genome annotation databases

KEGGitbr:Tb10.70.6650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10721 Unassigned DNA. Translation: AAA16328.1 .
PIRi S41631.

3D structure databases

ProteinModelPortali Q07010.
ModBasei Search...

Chemistry

BindingDBi Q07010.
ChEMBLi CHEMBL5069.

Proteomic databases

PRIDEi Q07010.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

KEGGi tbr:Tb10.70.6650.

Phylogenomic databases

KOi K00760.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .
BioCyci MetaCyc:MONOMER-15863.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma brucei."
    Allen T.E., Ullman B.
    Nucleic Acids Res. 21:5431-5438(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiHPRT_TRYBB
AccessioniPrimary (citable) accession number: Q07010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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