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Q07010

- HPRT_TRYBB

UniProt

Q07010 - HPRT_TRYBB

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HGPRT

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541GMPBy similarity
    Active sitei117 – 1171Proton acceptorBy similarity
    Binding sitei145 – 1451GMPBy similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Binding sitei173 – 1731GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi113 – 1219GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15863.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:HGPRT
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139596Add
    BLAST

    Proteomic databases

    PRIDEiQ07010.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ07010.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK00760.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07010-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPACKYDFA TSVLFTEAEL HTRMRGVAQR IADDYSNCNL KPLENPLVIV    50
    SVLKGSFVFT ADMVRILGDF GVPTRVEFLR ASSYGHDTKS CGRVDVKADG 100
    LCDIRGKHVL VLEDILDTAL TLREVVDSLK KSEPASIKTL VAIDKPGGRK 150
    IPFTAEYVVA DVPNVFVVGY GLDYDQSYRE VRDVVILKPS VYETWGKELE 200
    RRKAAGEAKR 210
    Length:210
    Mass (Da):23,360
    Last modified:June 1, 1994 - v1
    Checksum:i6134FA880F40EC3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10721 Unassigned DNA. Translation: AAA16328.1.
    PIRiS41631.

    Genome annotation databases

    KEGGitbr:Tb10.70.6650.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10721 Unassigned DNA. Translation: AAA16328.1 .
    PIRi S41631.

    3D structure databases

    ProteinModelPortali Q07010.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q07010.
    ChEMBLi CHEMBL5069.

    Proteomic databases

    PRIDEi Q07010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    KEGGi tbr:Tb10.70.6650.

    Phylogenomic databases

    KOi K00760.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci MetaCyc:MONOMER-15863.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma brucei."
      Allen T.E., Ullman B.
      Nucleic Acids Res. 21:5431-5438(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.

    Entry informationi

    Entry nameiHPRT_TRYBB
    AccessioniPrimary (citable) accession number: Q07010
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3