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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HGPRT

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54GMPBy similarity1
Active sitei117Proton acceptorBy similarity1
Binding sitei145GMPBy similarity1
Metal bindingi173MagnesiumBy similarity1
Binding sitei173GMP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 121GMPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15863.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HGPRT
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5069.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395961 – 210Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST210

Proteomic databases

PRIDEiQ07010.

Interactioni

Chemistry databases

BindingDBiQ07010.

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 15Combined sources6
Helixi17 – 34Combined sources18
Helixi36 – 38Combined sources3
Turni42 – 44Combined sources3
Beta strandi47 – 53Combined sources7
Helixi54 – 56Combined sources3
Helixi57 – 69Combined sources13
Beta strandi74 – 79Combined sources6
Beta strandi108 – 118Combined sources11
Helixi120 – 130Combined sources11
Beta strandi135 – 144Combined sources10
Helixi146 – 148Combined sources3
Beta strandi156 – 161Combined sources6
Beta strandi167 – 169Combined sources3
Beta strandi174 – 176Combined sources3
Beta strandi182 – 187Combined sources6
Helixi189 – 198Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JSQX-ray1.50A/B1-210[»]
5JV5X-ray2.73A/B1-210[»]
5K51X-ray2.96A/B/C/D1-210[»]
5KAMX-ray2.48A/C1-210[»]
5KAPX-ray2.95A/B1-210[»]
ProteinModelPortaliQ07010.
SMRiQ07010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
InterProiView protein in InterPro
IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPACKYDFA TSVLFTEAEL HTRMRGVAQR IADDYSNCNL KPLENPLVIV
60 70 80 90 100
SVLKGSFVFT ADMVRILGDF GVPTRVEFLR ASSYGHDTKS CGRVDVKADG
110 120 130 140 150
LCDIRGKHVL VLEDILDTAL TLREVVDSLK KSEPASIKTL VAIDKPGGRK
160 170 180 190 200
IPFTAEYVVA DVPNVFVVGY GLDYDQSYRE VRDVVILKPS VYETWGKELE
210
RRKAAGEAKR
Length:210
Mass (Da):23,360
Last modified:June 1, 1994 - v1
Checksum:i6134FA880F40EC3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10721 Unassigned DNA. Translation: AAA16328.1.
PIRiS41631.

Genome annotation databases

GeneDBiTb927.10.1400:pep.
Tb927.10.1470:pep.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHPRT_TRYBB
AccessioniPrimary (citable) accession number: Q07010
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 10, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families