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Protein

Glutamyl endopeptidase 2

Gene

sprE

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially cleaves peptide bonds on the carboxyl-terminal side of glutamate.

Catalytic activityi

Preferential cleavage: -Glu-|-Xaa- >> -Asp-|-Xaa-. Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-|-Asp- and -Glu-|-Pro- bonds is slow.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei33Charge relay systemBy similarity1
Active sitei62Charge relay systemBy similarity1
Active sitei143Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.267.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl endopeptidase 2 (EC:3.4.21.82)
Alternative name(s):
GLUSGP
Glutamic acid-specific protease
Glutamyl endopeptidase II
SGPE
Serine protease E
Streptogrisin-E
Gene namesi
Name:sprE
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938561 – 188Glutamyl endopeptidase 2Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi14 ↔ 34
Disulfide bondi137 ↔ 163

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi455632.SGR_1986.

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Beta strandi12 – 15Combined sources4
Beta strandi18 – 22Combined sources5
Beta strandi25 – 30Combined sources6
Helixi32 – 35Combined sources4
Beta strandi39 – 44Combined sources6
Beta strandi49 – 57Combined sources9
Beta strandi59 – 61Combined sources3
Beta strandi63 – 68Combined sources6
Beta strandi76 – 79Combined sources4
Beta strandi81 – 83Combined sources3
Beta strandi85 – 87Combined sources3
Beta strandi99 – 104Combined sources6
Turni105 – 107Combined sources3
Beta strandi108 – 123Combined sources16
Beta strandi126 – 134Combined sources9
Beta strandi146 – 149Combined sources4
Beta strandi152 – 161Combined sources10
Beta strandi171 – 174Combined sources4
Helixi175 – 182Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HPGX-ray1.50A1-187[»]
ProteinModelPortaliQ07006.
SMRiQ07006.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07006.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Family and domain databases

InterProiIPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
VLGGGAIYGG GSRCSAAFNV TKGGARYFVT AGHCTNISAN WSASSGGSVV
60 70 80 90 100
GVREGTSFPT NDYGIVRYTD GSSPAGTVDL YNGSTQDISS AANAVVGQAI
110 120 130 140 150
KKSGSTTKVT SGTVTAVNVT VNYGDGPVYN MGRTTACSAG GDSGGAHFAG
160 170 180
SVALGIHSGS SGCSGTAGSA IHQPVTKALS AYGVTVYL
Length:188
Mass (Da):18,337
Last modified:October 1, 1994 - v1
Checksum:i2762E5FDB2FD368D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67853 Genomic DNA. Translation: AAB20424.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67853 Genomic DNA. Translation: AAB20424.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HPGX-ray1.50A1-187[»]
ProteinModelPortaliQ07006.
SMRiQ07006.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_1986.

Protein family/group databases

MEROPSiS01.267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ07006.

Family and domain databases

InterProiIPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLUP_STRGR
AccessioniPrimary (citable) accession number: Q07006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.