Q07002 (CDK18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 18
EC=2.7.11.22

Alternative name(s):
Cell division protein kinase 18
PCTAIRE-motif protein kinase 3
Serine/threonine-protein kinase PCTAIRE-3

Gene names

Name:	CDK18
Synonyms:	PCTAIRE3, PCTK3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in signal transduction cascades in terminally differentiated cells.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Isoform 3 expression is limited to several subcortical nuclei of the basal gangli and the spinal cord. Isoform 2 is widely expressed. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Non-traceable author statement. Source: UniProtKB cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Non-traceable author statement. Source: UniProtKB signal transducer activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q07002-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q07002-2)

Also known as: 3a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MIM

Isoform 3 (identifier: Q07002-3)

Also known as: 3b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MIM
     89-89: E → EVRASGALPRQVAGCTHKGVHRRAAALQPDF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Cyclin-dependent kinase 18		PRO_0000086490
Regions
	Domain	142 – 423	282	Protein kinase
	Nucleotide binding	148 – 156	9	ATP By similarity
Sites
	Active site	263	1	Proton acceptor By similarity
	Binding site	171	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.8
	Modified residue	72	1	Phosphoserine Ref.7 Ref.9
	Modified residue	87	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	96	1	Phosphoserine Ref.9
	Modified residue	115	1	Phosphoserine Ref.7 Ref.9
	Modified residue	130	1	Phosphoserine Ref.8 Ref.10
Natural variations
	Alternative sequence	1	1	M → MIM in isoform 2 and isoform 3.		VSP_035888
	Alternative sequence	89	1	E → EVRASGALPRQVAGCTHKGV HRRAAALQPDF in isoform 3.		VSP_035889
	Natural variant	46	1	G → S. Ref.11		VAR_047802
	Natural variant	65	1	G → R. Ref.11		VAR_047803
	Natural variant	164	1	T → M. Ref.2 Ref.5 Ref.11		VAR_047804
Experimental info
	Sequence conflict	256	1	H → T in CAA47005. Ref.6
	Sequence conflict	321	1	D → A in CAA47005. Ref.6
	Sequence conflict	405	1	L → V in CAA47005. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 5CD938D3565BD01A	FASTA	472	54,180
	10 20 30 40 50 60 MNKMKNFKRR FSLSVPRTET IEESLAEFTE QFNQLHNRRN ENLQLGPLGR DPPQECSTFS 70 80 90 100 110 120 PTDSGEEPGQ LSPGVQFQRR QNQRRFSMED VSKRLSLPMD IRLPQEFLQK LQMESPDLPK 130 140 150 160 170 180 PLSRMSRRAS LSDIGFGKLE TYVKLDKLGE GTYATVFKGR SKLTENLVAL KEIRLEHEEG 190 200 210 220 230 240 APCTAIREVS LLKNLKHANI VTLHDLIHTD RSLTLVFEYL DSDLKQYLDH CGNLMSMHNV 250 260 270 280 290 300 KIFMFQLLRG LAYCHHRKIL HRDLKPQNLL INERGELKLA DFGLARAKSV PTKTYSNEVV 310 320 330 340 350 360 TLWYRPPDVL LGSTEYSTPI DMWGVGCIHY EMATGRPLFP GSTVKEELHL IFRLLGTPTE 370 380 390 400 410 420 ETWPGVTAFS EFRTYSFPCY LPQPLINHAP RLDTDGIHLL SSLLLYESKS RMSAEAALSH 430 440 450 460 470 SYFRSLGERV HQLEDTASIF SLKEIQLQKD PGYRGLAFQQ PGRGKNRRQS IF
	Isoform 2 (3a) [UniParc]. Checksum: 43C83A573A595476	FASTA	474	54,424
	10 20 30 40 50 60 MIMNKMKNFK RRFSLSVPRT ETIEESLAEF TEQFNQLHNR RNENLQLGPL GRDPPQECST 70 80 90 100 110 120 FSPTDSGEEP GQLSPGVQFQ RRQNQRRFSM EDVSKRLSLP MDIRLPQEFL QKLQMESPDL 130 140 150 160 170 180 PKPLSRMSRR ASLSDIGFGK LETYVKLDKL GEGTYATVFK GRSKLTENLV ALKEIRLEHE 190 200 210 220 230 240 EGAPCTAIRE VSLLKNLKHA NIVTLHDLIH TDRSLTLVFE YLDSDLKQYL DHCGNLMSMH 250 260 270 280 290 300 NVKIFMFQLL RGLAYCHHRK ILHRDLKPQN LLINERGELK LADFGLARAK SVPTKTYSNE 310 320 330 340 350 360 VVTLWYRPPD VLLGSTEYST PIDMWGVGCI HYEMATGRPL FPGSTVKEEL HLIFRLLGTP 370 380 390 400 410 420 TEETWPGVTA FSEFRTYSFP CYLPQPLINH APRLDTDGIH LLSSLLLYES KSRMSAEAAL 430 440 450 460 470 SHSYFRSLGE RVHQLEDTAS IFSLKEIQLQ KDPGYRGLAF QQPGRGKNRR QSIF
	Isoform 3 (3b) [UniParc]. Checksum: 174DA12243EDE359	FASTA	504	57,577
	10 20 30 40 50 60 MIMNKMKNFK RRFSLSVPRT ETIEESLAEF TEQFNQLHNR RNENLQLGPL GRDPPQECST 70 80 90 100 110 120 FSPTDSGEEP GQLSPGVQFQ RRQNQRRFSM EVRASGALPR QVAGCTHKGV HRRAAALQPD 130 140 150 160 170 180 FDVSKRLSLP MDIRLPQEFL QKLQMESPDL PKPLSRMSRR ASLSDIGFGK LETYVKLDKL 190 200 210 220 230 240 GEGTYATVFK GRSKLTENLV ALKEIRLEHE EGAPCTAIRE VSLLKNLKHA NIVTLHDLIH 250 260 270 280 290 300 TDRSLTLVFE YLDSDLKQYL DHCGNLMSMH NVKIFMFQLL RGLAYCHHRK ILHRDLKPQN 310 320 330 340 350 360 LLINERGELK LADFGLARAK SVPTKTYSNE VVTLWYRPPD VLLGSTEYST PIDMWGVGCI 370 380 390 400 410 420 HYEMATGRPL FPGSTVKEEL HLIFRLLGTP TEETWPGVTA FSEFRTYSFP CYLPQPLINH 430 440 450 460 470 480 APRLDTDGIH LLSSLLLYES KSRMSAEAAL SHSYFRSLGE RVHQLEDTAS IFSLKEIQLQ 490 500 KDPGYRGLAF QQPGRGKNRR QSIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression analysis of two novel PCTAIRE 3 transcripts from human brain." Herskovits A.Z., Davies P. Gene 328:59-67(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY. Tissue: Hippocampus.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-164.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-164. Tissue: Colon.
[6]	"A family of human cdc2-related protein kinases." Meyerson M., Enders G.H., Wu C.-L., Su L.-K., Gorka C., Nelson C., Harlow E., Tsai L.-H. EMBO J. 11:2909-2917(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 93-472.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-87 AND SER-115, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-87 AND SER-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-87; SER-96 AND SER-115, MASS SPECTROMETRY.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, MASS SPECTROMETRY.
[11]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-46; ARG-65 AND MET-164.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY353237 mRNA. Translation: AAR13065.1. AY353238 mRNA. Translation: AAR13066.1. BT007299 mRNA. Translation: AAP35963.1. AL357131 Genomic DNA. Translation: CAH72012.1. CH471067 Genomic DNA. Translation: EAW91559.1. CH471067 Genomic DNA. Translation: EAW91560.1. BC011526 mRNA. Translation: AAH11526.1. X66362 mRNA. Translation: CAA47005.1.
IPI	IPI00394661. IPI00414292. IPI00914963.
PIR	S32831.
RefSeq	NP_002587.2. NM_002596.3. NP_997667.1. NM_212502.2. NP_997668.1. NM_212503.2.
UniGene	Hs.445402.
3D structure databases
ProteinModelPortal	Q07002.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07002. 7 interactions.
MINT	MINT-3294654.
STRING	9606.ENSP00000342589.
PTM databases
PhosphoSite	Q07002.
Polymorphism databases
DMDM	116242704.
Proteomic databases
PaxDb	Q07002.
PRIDE	Q07002.
Protocols and materials databases
DNASU	5129.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360066; ENSP00000353176; ENSG00000117266. ENST00000429964; ENSP00000399082; ENSG00000117266. ENST00000506784; ENSP00000423665; ENSG00000117266.
GeneID	5129.
KEGG	hsa:5129.
UCSC	uc001hcp.3. human. uc001hcr.3. human. uc001hcs.3. human.
Organism-specific databases
CTD	5129.
GeneCards	GC01P205474.
HGNC	HGNC:8751. CDK18.
HPA	HPA045429.
MIM	169190. gene.
neXtProt	NX_Q07002.
PharmGKB	PA33097.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
KO	K15596.
OMA	HTDRSLT.
Gene expression databases
ArrayExpress	Q07002.
Bgee	Q07002.
CleanEx	HS_PCTK3.
Genevestigator	Q07002.
GermOnline	ENSG00000117266. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q07002.
ChEMBL	CHEMBL5316.
ChiTaRS	CDK18. human.
GenomeRNAi	5129.
NextBio	19772.
SOURCE	Search...

Entry information

Entry name

CDK18_HUMAN

Accession

Primary (citable) accession number: Q07002
Secondary accession number(s): Q5VXQ2 , Q6V3A2, Q6V3A3, Q96F90

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 131 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.