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Protein

5-hydroxyisourate hydrolase

Gene

urah

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).

Catalytic activityi

5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.1 Publication

Pathwayi: urate degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (uox)
  2. 5-hydroxyisourate hydrolase (urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateBy similarity1
Binding sitei71SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • purine nucleobase metabolic process Source: ZFIN
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BRENDAi3.5.2.17. 928.
UniPathwayiUPA00394; UER00651.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxyisourate hydrolase (EC:3.5.2.17)
Short name:
HIU hydrolase
Short name:
HIUHase
Alternative name(s):
Transthyretin-related protein
Gene namesi
Name:urah
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-060825-253. urah.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: ZFIN
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69D → A: Reduces activity by 50%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003152831 – 1385-hydroxyisourate hydrolaseAdd BLAST138

Proteomic databases

PaxDbiQ06S87.

Expressioni

Gene expression databases

ExpressionAtlasiQ06S87. differential.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090143.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 29Combined sources3
Beta strandi32 – 34Combined sources3
Turni35 – 38Combined sources4
Beta strandi45 – 51Combined sources7
Beta strandi53 – 55Combined sources3
Beta strandi58 – 65Combined sources8
Turni78 – 80Combined sources3
Beta strandi83 – 90Combined sources8
Helixi92 – 98Combined sources7
Beta strandi108 – 114Combined sources7
Beta strandi125 – 128Combined sources4
Beta strandi131 – 135Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H1XX-ray1.98A/B/C/D22-138[»]
2H6UX-ray1.70A/B/C/D/E/F/G/H22-138[»]
3IWUX-ray2.30A/B/C/D/E/F/G/H1-138[»]
3IWVX-ray1.68A/B/C/D1-138[»]
3Q1EX-ray1.95A/B/C/D20-138[»]
ProteinModelPortaliQ06S87.
SMRiQ06S87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06S87.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3006. Eukaryota.
COG2351. LUCA.
GeneTreeiENSGT00390000005321.
HOGENOMiHOG000251776.
InParanoidiQ06S87.
PhylomeDBiQ06S87.

Family and domain databases

Gene3Di2.60.40.180. 1 hit.
InterProiIPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
[Graphical view]
PfamiPF00576. Transthyretin. 1 hit.
[Graphical view]
PRINTSiPR00189. TRNSTHYRETIN.
SMARTiSM00095. TR_THY. 1 hit.
[Graphical view]
SUPFAMiSSF49472. SSF49472. 1 hit.
TIGRFAMsiTIGR02962. hdxy_isourate. 1 hit.
PROSITEiPS00768. TRANSTHYRETIN_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q06S87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRLQHIRGH IVSADKHINM SATLLSPLST HVLNIAQGVP GANMTIVLHR
60 70 80 90 100
LDPVSSAWNI LTTGITNDDG RCPGLITKEN FIAGVYKMRF ETGKYWDALG
110 120 130
ETCFYPYVEI VFTITNTSQH YHVPLLLSRF SYSTYRGS
Length:138
Mass (Da):15,486
Last modified:October 31, 2006 - v1
Checksum:iBC8EEBCFF5116862
GO
Isoform 2 (identifier: Q06S87-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:95
Mass (Da):10,890
Checksum:iD9E1BD36A374F1C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0305241 – 43Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ640307 mRNA. Translation: ABG21128.1.
BC122322 mRNA. Translation: AAI22323.1.
RefSeqiXP_009291914.1. XM_009293639.2. [Q06S87-2]
UniGeneiDr.91711.

Genome annotation databases

EnsembliENSDART00000163471; ENSDARP00000138143; ENSDARG00000100719. [Q06S87-2]
GeneIDi751742.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ640307 mRNA. Translation: ABG21128.1.
BC122322 mRNA. Translation: AAI22323.1.
RefSeqiXP_009291914.1. XM_009293639.2. [Q06S87-2]
UniGeneiDr.91711.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H1XX-ray1.98A/B/C/D22-138[»]
2H6UX-ray1.70A/B/C/D/E/F/G/H22-138[»]
3IWUX-ray2.30A/B/C/D/E/F/G/H1-138[»]
3IWVX-ray1.68A/B/C/D1-138[»]
3Q1EX-ray1.95A/B/C/D20-138[»]
ProteinModelPortaliQ06S87.
SMRiQ06S87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090143.

Proteomic databases

PaxDbiQ06S87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000163471; ENSDARP00000138143; ENSDARG00000100719. [Q06S87-2]
GeneIDi751742.

Organism-specific databases

CTDi76974.
ZFINiZDB-GENE-060825-253. urah.

Phylogenomic databases

eggNOGiKOG3006. Eukaryota.
COG2351. LUCA.
GeneTreeiENSGT00390000005321.
HOGENOMiHOG000251776.
InParanoidiQ06S87.
PhylomeDBiQ06S87.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00651.
BRENDAi3.5.2.17. 928.

Miscellaneous databases

EvolutionaryTraceiQ06S87.
PROiQ06S87.

Gene expression databases

ExpressionAtlasiQ06S87. differential.

Family and domain databases

Gene3Di2.60.40.180. 1 hit.
InterProiIPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
[Graphical view]
PfamiPF00576. Transthyretin. 1 hit.
[Graphical view]
PRINTSiPR00189. TRNSTHYRETIN.
SMARTiSM00095. TR_THY. 1 hit.
[Graphical view]
SUPFAMiSSF49472. SSF49472. 1 hit.
TIGRFAMsiTIGR02962. hdxy_isourate. 1 hit.
PROSITEiPS00768. TRANSTHYRETIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIUH_DANRE
AccessioniPrimary (citable) accession number: Q06S87
Secondary accession number(s): Q0P422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

HIU hydrolysis also occurs spontaneously, but more slowly.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.