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Protein

5-hydroxyisourate hydrolase

Gene

urah

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).

Catalytic activityi

5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.1 Publication

Pathwayi: urate degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (uox)
  2. 5-hydroxyisourate hydrolase (urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311SubstrateBy similarity
Binding sitei71 – 711SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • purine nucleobase metabolic process Source: ZFIN
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BRENDAi3.5.2.17. 928.
UniPathwayiUPA00394; UER00651.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxyisourate hydrolase (EC:3.5.2.17)
Short name:
HIU hydrolase
Short name:
HIUHase
Alternative name(s):
Transthyretin-related protein
Gene namesi
Name:urah
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-060825-253. urah.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: ZFIN
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691D → A: Reduces activity by 50%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1381385-hydroxyisourate hydrolasePRO_0000315283Add
BLAST

Proteomic databases

PaxDbiQ06S87.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090143.

Structurei

Secondary structure

1
138
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 293Combined sources
Beta strandi32 – 343Combined sources
Turni35 – 384Combined sources
Beta strandi45 – 517Combined sources
Beta strandi53 – 553Combined sources
Beta strandi58 – 658Combined sources
Turni78 – 803Combined sources
Beta strandi83 – 908Combined sources
Helixi92 – 987Combined sources
Beta strandi108 – 1147Combined sources
Beta strandi125 – 1284Combined sources
Beta strandi131 – 1355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H1XX-ray1.98A/B/C/D22-138[»]
2H6UX-ray1.70A/B/C/D/E/F/G/H22-138[»]
3IWUX-ray2.30A/B/C/D/E/F/G/H1-138[»]
3IWVX-ray1.68A/B/C/D1-138[»]
3Q1EX-ray1.95A/B/C/D20-138[»]
ProteinModelPortaliQ06S87.
SMRiQ06S87. Positions 25-138.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06S87.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3006. Eukaryota.
COG2351. LUCA.
GeneTreeiENSGT00390000005321.
HOGENOMiHOG000251776.
InParanoidiQ06S87.
PhylomeDBiQ06S87.

Family and domain databases

Gene3Di2.60.40.180. 1 hit.
InterProiIPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
[Graphical view]
PfamiPF00576. Transthyretin. 1 hit.
[Graphical view]
PRINTSiPR00189. TRNSTHYRETIN.
SMARTiSM00095. TR_THY. 1 hit.
[Graphical view]
SUPFAMiSSF49472. SSF49472. 1 hit.
TIGRFAMsiTIGR02962. hdxy_isourate. 1 hit.
PROSITEiPS00768. TRANSTHYRETIN_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q06S87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRLQHIRGH IVSADKHINM SATLLSPLST HVLNIAQGVP GANMTIVLHR
60 70 80 90 100
LDPVSSAWNI LTTGITNDDG RCPGLITKEN FIAGVYKMRF ETGKYWDALG
110 120 130
ETCFYPYVEI VFTITNTSQH YHVPLLLSRF SYSTYRGS
Length:138
Mass (Da):15,486
Last modified:October 31, 2006 - v1
Checksum:iBC8EEBCFF5116862
GO
Isoform 2 (identifier: Q06S87-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:95
Mass (Da):10,890
Checksum:iD9E1BD36A374F1C1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4343Missing in isoform 2. 1 PublicationVSP_030524Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ640307 mRNA. Translation: ABG21128.1.
BC122322 mRNA. Translation: AAI22323.1.
RefSeqiXP_009291914.1. XM_009293639.1. [Q06S87-2]
UniGeneiDr.91711.

Genome annotation databases

EnsembliENSDART00000163471; ENSDARP00000138143; ENSDARG00000100719. [Q06S87-2]
GeneIDi751742.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ640307 mRNA. Translation: ABG21128.1.
BC122322 mRNA. Translation: AAI22323.1.
RefSeqiXP_009291914.1. XM_009293639.1. [Q06S87-2]
UniGeneiDr.91711.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H1XX-ray1.98A/B/C/D22-138[»]
2H6UX-ray1.70A/B/C/D/E/F/G/H22-138[»]
3IWUX-ray2.30A/B/C/D/E/F/G/H1-138[»]
3IWVX-ray1.68A/B/C/D1-138[»]
3Q1EX-ray1.95A/B/C/D20-138[»]
ProteinModelPortaliQ06S87.
SMRiQ06S87. Positions 25-138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090143.

Proteomic databases

PaxDbiQ06S87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000163471; ENSDARP00000138143; ENSDARG00000100719. [Q06S87-2]
GeneIDi751742.

Organism-specific databases

CTDi76974.
ZFINiZDB-GENE-060825-253. urah.

Phylogenomic databases

eggNOGiKOG3006. Eukaryota.
COG2351. LUCA.
GeneTreeiENSGT00390000005321.
HOGENOMiHOG000251776.
InParanoidiQ06S87.
PhylomeDBiQ06S87.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00651.
BRENDAi3.5.2.17. 928.

Miscellaneous databases

EvolutionaryTraceiQ06S87.
PROiQ06S87.

Family and domain databases

Gene3Di2.60.40.180. 1 hit.
InterProiIPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
[Graphical view]
PfamiPF00576. Transthyretin. 1 hit.
[Graphical view]
PRINTSiPR00189. TRNSTHYRETIN.
SMARTiSM00095. TR_THY. 1 hit.
[Graphical view]
SUPFAMiSSF49472. SSF49472. 1 hit.
TIGRFAMsiTIGR02962. hdxy_isourate. 1 hit.
PROSITEiPS00768. TRANSTHYRETIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIUH_DANRE
AccessioniPrimary (citable) accession number: Q06S87
Secondary accession number(s): Q0P422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 31, 2006
Last modified: June 8, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

HIU hydrolysis also occurs spontaneously, but more slowly.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.