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Protein

5-hydroxyisourate hydrolase

Gene

urah

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).

Miscellaneous

HIU hydrolysis also occurs spontaneously, but more slowly.

Catalytic activityi

5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.1 Publication

Pathwayi: urate degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (uox)
  2. 5-hydroxyisourate hydrolase (urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateBy similarity1
Binding sitei71SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • purine nucleobase metabolic process Source: ZFIN
  • urate catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processPurine metabolism

Enzyme and pathway databases

BRENDAi3.5.2.17. 928.
UniPathwayiUPA00394; UER00651.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxyisourate hydrolase (EC:3.5.2.17)
Short name:
HIU hydrolase
Short name:
HIUHase
Alternative name(s):
Transthyretin-related protein
Gene namesi
Name:urah
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-060825-253. uraha.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69D → A: Reduces activity by 50%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003152831 – 1385-hydroxyisourate hydrolaseAdd BLAST138

Proteomic databases

PaxDbiQ06S87.

Expressioni

Gene expression databases

BgeeiENSDARG00000100719.
ExpressionAtlasiQ06S87. baseline.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090143.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 29Combined sources3
Beta strandi32 – 34Combined sources3
Turni35 – 38Combined sources4
Beta strandi45 – 51Combined sources7
Beta strandi53 – 55Combined sources3
Beta strandi58 – 65Combined sources8
Turni78 – 80Combined sources3
Beta strandi83 – 90Combined sources8
Helixi92 – 98Combined sources7
Beta strandi108 – 114Combined sources7
Beta strandi125 – 128Combined sources4
Beta strandi131 – 135Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H1XX-ray1.98A/B/C/D22-138[»]
2H6UX-ray1.70A/B/C/D/E/F/G/H22-138[»]
3IWUX-ray2.30A/B/C/D/E/F/G/H1-138[»]
3IWVX-ray1.68A/B/C/D1-138[»]
3Q1EX-ray1.95A/B/C/D20-138[»]
ProteinModelPortaliQ06S87.
SMRiQ06S87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06S87.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3006. Eukaryota.
COG2351. LUCA.
GeneTreeiENSGT00390000005321.
HOGENOMiHOG000251776.
InParanoidiQ06S87.
PhylomeDBiQ06S87.

Family and domain databases

Gene3Di2.60.40.180. 1 hit.
InterProiView protein in InterPro
IPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_d.
IPR036817. Transthyretin/HIU_hydrolase_sf.
PANTHERiPTHR10395. PTHR10395. 1 hit.
PfamiView protein in Pfam
PF00576. Transthyretin. 1 hit.
PRINTSiPR00189. TRNSTHYRETIN.
SMARTiView protein in SMART
SM00095. TR_THY. 1 hit.
SUPFAMiSSF49472. SSF49472. 1 hit.
TIGRFAMsiTIGR02962. hdxy_isourate. 1 hit.
PROSITEiView protein in PROSITE
PS00768. TRANSTHYRETIN_1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q06S87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRLQHIRGH IVSADKHINM SATLLSPLST HVLNIAQGVP GANMTIVLHR
60 70 80 90 100
LDPVSSAWNI LTTGITNDDG RCPGLITKEN FIAGVYKMRF ETGKYWDALG
110 120 130
ETCFYPYVEI VFTITNTSQH YHVPLLLSRF SYSTYRGS
Length:138
Mass (Da):15,486
Last modified:October 31, 2006 - v1
Checksum:iBC8EEBCFF5116862
GO
Isoform 2 (identifier: Q06S87-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:95
Mass (Da):10,890
Checksum:iD9E1BD36A374F1C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0305241 – 43Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ640307 mRNA. Translation: ABG21128.1.
BC122322 mRNA. Translation: AAI22323.1.
RefSeqiXP_009291914.1. XM_009293639.2.
UniGeneiDr.91711.

Genome annotation databases

EnsembliENSDART00000163471; ENSDARP00000138143; ENSDARG00000100719. [Q06S87-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHIUH_DANRE
AccessioniPrimary (citable) accession number: Q06S87
Secondary accession number(s): Q0P422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 31, 2006
Last modified: November 22, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families