Reviewed, UniProtKB/Swiss-Prot Q06PW6 (NRFA_PASHA)

Names and origin

Protein names

Recommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase

Gene names

Name:

nrfA

Organism

Pasteurella haemolytica (Mannheimia haemolytica)

Taxonomic identifier

75985 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in nitrite reduction By similarity.
Catalytic activity	NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182
Cofactor	Binds 1 calcium ion per monomer By similarity. Binds 5 heme groups covalently per monomer By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the cytochrome c-552 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen compound metabolic process Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro nitrite reductase (cytochrome, ammonia-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 500	476	Cytochrome c-552 HAMAP MF_01182		PRO_0000268969
Sites
	Metal binding	102	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	134	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	172	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	221	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	223	1	Calcium By similarity
	Metal binding	224	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	268	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	270	1	Calcium By similarity
	Metal binding	282	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	293	1	Iron (heme 4 axial ligand) By similarity
	Metal binding	308	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	325	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	400	1	Iron (heme 4 axial ligand) By similarity
	Binding site	130	1	Heme 1 (covalent) By similarity
	Binding site	133	1	Heme 1 (covalent) By similarity
	Binding site	168	1	Heme 2 (covalent) By similarity
	Binding site	171	1	Heme 2 (covalent) By similarity
	Binding site	217	1	Heme 3 (covalent) By similarity
	Binding site	220	1	Heme 3 (covalent) By similarity
	Binding site	224	1	Substrate By similarity
	Binding site	271	1	Substrate By similarity
	Binding site	289	1	Heme 4 (covalent) By similarity
	Binding site	292	1	Heme 4 (covalent) By similarity
	Binding site	321	1	Heme 5 (covalent) By similarity
	Binding site	324	1	Heme 5 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06PW6-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 83F4D8702BC3D7DD	FASTA	500	56,129
	10 20 30 40 50 60 MKFLIKSLAV ATISILGCLQ TALAEEAKTE SLTDKAVKHE KLGVKIESAN HLFAEKYPLQ 70 80 90 100 110 120 YDSWKSTAKS TDRGSALEAD PRYVILWAGY AFAKDYNKPR GHFYAVTDVR DILRTGAPKD 130 140 150 160 170 180 ENDGPQPMAC WTCKGPDVPR LIEEKGERGY FDPKWAKYGA EIVNSIGCAD CHDTTSEEFK 190 200 210 220 230 240 QGKPALRVAR PHVLRALNTV GWKFEDLDKH GKRPAVCANC HVEYYFKNKT DVTFPWDKGV 250 260 270 280 290 300 DVDSIEKYYD EINFTDWTHA LSKAPMLKTQ HPDFEVWSQG THGKNGVTCI DCHMPKVKDK 310 320 330 340 350 360 DGKVYTDHKI GNPFDQFEAT CKTCHEQSKE TLEKRVKEYK HEVKEAMIRL EDQLVKAHFE 370 380 390 400 410 420 AKAAWEAGAT QEEMKEILMA IRHAQWRWDY SAAGHGNHFH APDVMLRTIA TGIDRAADAR 430 440 450 460 470 480 AKLGVVLAKH GVTTPVAIPD ISTKEKAQLA IGLDIPKEQA AKDEFLKTVV PQWEKEARAK 490 500 GLLPAEEADK PVAAPKAEAK

References

[1]	Roehrig S.C., Tran H.Q., Spehr V., Gunkel N., Selzer P.M., Ullrich H.J. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Serotype A1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ680244 Genomic DNA. Translation: ABG89201.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.2.2. 267636.
Family and domain databases
HAMAP	MF_01182. [Tree]
InterPro	IPR003321. Cyt_c552. IPR017570. Cyt_c_NO2Rdtase_formate-dep. IPR011031. Multihaem_cyt. [Graphical view]
Pfam	PF02335. Cytochrom_C552. 1 hit. [Graphical view]
PIRSF	PIRSF000243. Cyt_c552. 1 hit.
TIGRFAMs	TIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFA_PASHA

Accession

Primary (citable) accession number: Q06PW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)