Reviewed, UniProtKB/Swiss-Prot Q06J43 (CYB6_BIGNA)

Names and origin

Protein names

Recommended name:
    Cytochrome b6

Gene names

Name:	petB-A
AND
Name:	petB-B

Encoded on

Plastid; Chloroplast

Organism

Bigelowiella natans (Pedinomonas minutissima) (Chlorarachnion sp. (strain CCMP621))

Taxonomic identifier

227086 [NCBI]

Taxonomic lineage

Eukaryota › Rhizaria › Cercozoa › Chlorarachniophyceae › Bigelowiella

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Cofactor	Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity.
Miscellaneous	Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm By similarity.
Sequence similarities	Belongs to the cytochrome b family. PetB subfamily.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	photosynthesis Inferred from electronic annotation. Source: HAMAP respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Cytochrome b6 HAMAP MF_00633		PRO_0000296339
Regions
	Transmembrane	32 – 52	21	Potential
	Transmembrane	90 – 110	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	186 – 206	21	Potential
Sites
	Metal binding	86	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	100	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	187	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	202	1	Iron (heme 1 axial ligand) By similarity
	Binding site	35	1	Heme 1 (covalent; via 1 link) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06J43-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 3326AD9D74DB2357	FASTA	215	23,925
	10 20 30 40 50 60 MAVIYNWFND RLEIQAVADD ITAKYVPPHV NLFYCLGGIT LTCFLIQVAT GFALTFYYRP 70 80 90 100 110 120 TVGEALSSVR SIMLDTNFGW LIRSVHRWCA SMMVLMMVLH VFRVYLTGGF KNPRESTWVT 130 140 150 160 170 180 GVIMASCTVS FGVTGYSLPW DQVGYWAVKI VTGVPEAIPV IGPAIVQLLR GNSSVGQATL 190 200 210 TRFYSLHTFV LPLLTAVFML GHFLMIRKQG ISGPL

References

[1]

"The complete chloroplast genome of the chlorarachniophyte Bigelowiella natans: evidence for independent origins of chlorarachniophyte and euglenid secondary endosymbionts." Rogers M.B., Gilson P.R., Su V., McFadden G.I., Keeling P.J. Mol. Biol. Evol. 24:54-62(2007) [PubMed: 16990439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ851108 Genomic DNA. Translation: ABG91409.1. DQ851108 Genomic DNA. Translation: ABG91416.1.
RefSeq	YP_778577.1. YP_778584.1.
3D structure databases
SMR	Q06J43. Positions 4-215.
ModBase	Search...
Genome annotation databases
GeneID	4352994. 4353001.
Family and domain databases
HAMAP	MF_00633. [Tree]
InterPro	IPR016175. Cyt_b/b6. IPR005797. Cyt_b/b6_N. [Graphical view]
Gene3D	G3DSA:1.20.810.10. Cytochrome_b/b6. 1 hit.
PANTHER	PTHR19271. Cytochrome_b/b6. 1 hit.
Pfam	PF00033. Cytochrom_B_N. 1 hit. [Graphical view]
PROSITE	PS51002. CYTB_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB6_BIGNA

Accession

Primary (citable) accession number: Q06J43

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)