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Q06HQ7

- PLB1_MONDO

UniProt

Q06HQ7 - PLB1_MONDO

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Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031NucleophileBy similarity
Binding sitei477 – 4771Substrate; via amide nitrogenBy similarity
Binding sitei516 – 5161SubstrateBy similarity
Active sitei1361 – 13611By similarity
Active sitei1364 – 13641By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
Synonyms:PLB
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
ProteomesiUP000002280: Unplaced

Subcellular locationi

Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes.By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 14741452Phospholipase B1, membrane-associatedPRO_0000324384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi711 – 7111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1070 – 10701N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi13616.ENSMODP00000019434.

Structurei

3D structure databases

ProteinModelPortaliQ06HQ7.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 14321410ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1454 – 147421CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1433 – 145321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati36 – 3503151Add
BLAST
Repeati365 – 7103462Add
BLAST
Repeati711 – 10693593Add
BLAST
Repeati1079 – 14173394Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 141713824 X 308-326 AA approximate repeatsAdd
BLAST
Regioni1418 – 146144Necessary for membrane localizationBy similarityAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ06HQ7.
KOiK14621.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06HQ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLQPSLLLL ALLLLKVTTI QATLGNNSWE EQTWPEGLKN FPFPCRPEEI
60 70 80 90 100
PFSPPSESVH SLRISDIRVV GAIGNLETTP DAKMVLKRQK WIERNQMQTC
110 120 130 140 150
MQVMTVLSDF IKKFSPSVLP LLCPSREKVL PRMRNEDLGP QARELVQHMK
160 170 180 190 200
KNQKLNFRND WKLINVFFNA QSQCHPCSSS QKEGNMTSSM QELIRLLDYL
210 220 230 240 250
QKEVPKAFVN LVDISELGNS IQSNNQENGP RDINEVCECP QEPSRLTTAV
260 270 280 290 300
QRWYYQNSWE KLLTSGRYDT KDTFTVIYQS FLHEMEPSVF PIKQTSGSSL
310 320 330 340 350
KKLSTALAVS LWNGMIDPTG KKEEFFTTGQ RIPVKCPTQK HPYLFTYRNN
360 370 380 390 400
NKLSSTPEIK RISETREGTE IRCPDMDPSN AVPTSVHHLR PADIKVIGAL
410 420 430 440 450
GDSLTAGNGA GSTPGNILDV LTQYRGLSWS IGGNENISTV TTLANILREF
460 470 480 490 500
NPSLIGFSTG TGNQNSAAAF LNQAVAGATS ETLPSQARRL VDLMKENKRI
510 520 530 540 550
NFQDDWKIVT LFIGGNDLCD SCSDPPRYSS QNFTAHIGKA LDILHAEVPR
560 570 580 590 600
VFVNLVTVLS IVSLRELYQE ESINCPRVIL RSLCPCVLKF KDNSTELTTL
610 620 630 640 650
IELNKQYQEE THRLVDSGRY DTRDDFTVVL QPFLEQVPMP KTPEGLPDSS
660 670 680 690 700
FFAPDCFHFH SKAHARVARS LWNNMLEPVG EKKKEENLEN IVDLICPSES
710 720 730 740 750
QPYLRTYKNS NYTYPTATPT SSPKPDYGSQ LWCEDRAPSA SFPTSVHALK
760 770 780 790 800
PADIQVVAAV GDSLTAGNGI GSKPDDLPDV ITQYRGLSYS AGGDASLESV
810 820 830 840 850
TTLPNILREF NMNLTGFAVG VGSADDANAF LNQAVPGAKA EGLKKQVQTL
860 870 880 890 900
VQKMKSDPRI NFQRDWKVIT LLIGTTDLCD YCTDSNLYSA TNFSAYLRDA
910 920 930 940 950
LDFLHREVPR ALVNLVDFMN PIIFRQVFLG NQDKCPMTQA CSLCNCLLTV
960 970 980 990 1000
REDSPELARM KEVVKVYQTK IRELVESGRY DTREDFTIVL QPFLEEVKLA
1010 1020 1030 1040 1050
FQDGIPDVSF FAPDCIHPNQ KFHSQLSRAL WNNMLQPLGK KMDFLDLTAD
1060 1070 1080 1090 1100
TTLSCPSLEE PFLRTYRNSN YTYPANPAIE NWGSDFLCPE QGSSNSAPSS
1110 1120 1130 1140 1150
VHQLRPADIK VVAALGDSLT TAVGAGAKNN SNWSTAWRGL SWSIGGDGTL
1160 1170 1180 1190 1200
ETHTTLPNIL KKFNPHLHGF STGTQEEAAG LNVAVEGAIA QDMPSQARIL
1210 1220 1230 1240 1250
VDRMKNSSEI NLDQDWKLIT IFVGSNDLCQ YCENPEAHSV KNYVQSLQQA
1260 1270 1280 1290 1300
LDIFYKELPR AFINVVEIME LVGLRQIQGE KCAVTTQSIC PCFGRSLDNS
1310 1320 1330 1340 1350
PELQEMKNIN RHFQSGSSLI TYHHQYMERE DFAVVVQPFF QSTVVPLDDK
1360 1370 1380 1390 1400
GKPDLSFFSV DCFHFSERGH AEMAIALWNN MLEPVGQKQT YNNFTHSRTK
1410 1420 1430 1440 1450
VKCPTSENPY FFTMRNSGLL RKEASHKTPS VPFWAVLVAA AASFIVGVVL
1460 1470
VMLWRTKKCS RREERPISLV ATVF
Length:1,474
Mass (Da):165,157
Last modified:October 31, 2006 - v1
Checksum:iB22844CD69B35A60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ875604 mRNA. Translation: ABI74623.1.
RefSeqiNP_001070653.1. NM_001077185.1.
UniGeneiMdm.186.

Genome annotation databases

GeneIDi768082.
KEGGimdo:768082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ875604 mRNA. Translation: ABI74623.1 .
RefSeqi NP_001070653.1. NM_001077185.1.
UniGenei Mdm.186.

3D structure databases

ProteinModelPortali Q06HQ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 13616.ENSMODP00000019434.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 768082.
KEGGi mdo:768082.

Organism-specific databases

CTDi 5350.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi Q06HQ7.
KOi K14621.

Family and domain databases

Gene3Di 3.40.50.1110. 6 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dietary cholesterol increases intestinal phospholipase B gene expression in laboratory opossums."
    Chan J., Kushwaha R.S., Gluhak-Heinrich J., Donalson L.M., VandeBerg J.F., VandeBerg J.L.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPLB1_MONDO
AccessioniPrimary (citable) accession number: Q06HQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3