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Q06HQ7

- PLB1_MONDO

UniProt

Q06HQ7 - PLB1_MONDO

Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.By similarity

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by diisopropyl fluorophosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei403 – 4031NucleophileBy similarity
    Binding sitei477 – 4771Substrate; via amide nitrogenBy similarity
    Binding sitei516 – 5161SubstrateBy similarity
    Active sitei1361 – 13611By similarity
    Active sitei1364 – 13641By similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC
    2. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B1, membrane-associated
    Short name:
    Phospholipase B
    Alternative name(s):
    Phospholipase B/lipase
    Short name:
    PLB/LIP
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:PLB1
    Synonyms:PLB
    OrganismiMonodelphis domestica (Gray short-tailed opossum)
    Taxonomic identifieri13616 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
    ProteomesiUP000002280: Unplaced

    Subcellular locationi

    Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Present in the intestinal brush border membranes.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 14741452Phospholipase B1, membrane-associatedPRO_0000324384Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi711 – 7111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1070 – 10701N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes proteolytic cleavage in the ileum.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi13616.ENSMODP00000019434.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06HQ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 14321410ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1454 – 147421CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1433 – 145321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati36 – 3503151Add
    BLAST
    Repeati365 – 7103462Add
    BLAST
    Repeati711 – 10693593Add
    BLAST
    Repeati1079 – 14173394Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 141713824 X 308-326 AA approximate repeatsAdd
    BLAST
    Regioni1418 – 146144Necessary for membrane localizationBy similarityAdd
    BLAST

    Domaini

    Repeat 2 contains the catalytic domain.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311176.
    HOGENOMiHOG000115574.
    HOVERGENiHBG108263.
    InParanoidiQ06HQ7.
    KOiK14621.

    Family and domain databases

    Gene3Di3.40.50.1110. 6 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 3 hits.
    [Graphical view]
    PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06HQ7-1 [UniParc]FASTAAdd to Basket

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    MRLQPSLLLL ALLLLKVTTI QATLGNNSWE EQTWPEGLKN FPFPCRPEEI     50
    PFSPPSESVH SLRISDIRVV GAIGNLETTP DAKMVLKRQK WIERNQMQTC 100
    MQVMTVLSDF IKKFSPSVLP LLCPSREKVL PRMRNEDLGP QARELVQHMK 150
    KNQKLNFRND WKLINVFFNA QSQCHPCSSS QKEGNMTSSM QELIRLLDYL 200
    QKEVPKAFVN LVDISELGNS IQSNNQENGP RDINEVCECP QEPSRLTTAV 250
    QRWYYQNSWE KLLTSGRYDT KDTFTVIYQS FLHEMEPSVF PIKQTSGSSL 300
    KKLSTALAVS LWNGMIDPTG KKEEFFTTGQ RIPVKCPTQK HPYLFTYRNN 350
    NKLSSTPEIK RISETREGTE IRCPDMDPSN AVPTSVHHLR PADIKVIGAL 400
    GDSLTAGNGA GSTPGNILDV LTQYRGLSWS IGGNENISTV TTLANILREF 450
    NPSLIGFSTG TGNQNSAAAF LNQAVAGATS ETLPSQARRL VDLMKENKRI 500
    NFQDDWKIVT LFIGGNDLCD SCSDPPRYSS QNFTAHIGKA LDILHAEVPR 550
    VFVNLVTVLS IVSLRELYQE ESINCPRVIL RSLCPCVLKF KDNSTELTTL 600
    IELNKQYQEE THRLVDSGRY DTRDDFTVVL QPFLEQVPMP KTPEGLPDSS 650
    FFAPDCFHFH SKAHARVARS LWNNMLEPVG EKKKEENLEN IVDLICPSES 700
    QPYLRTYKNS NYTYPTATPT SSPKPDYGSQ LWCEDRAPSA SFPTSVHALK 750
    PADIQVVAAV GDSLTAGNGI GSKPDDLPDV ITQYRGLSYS AGGDASLESV 800
    TTLPNILREF NMNLTGFAVG VGSADDANAF LNQAVPGAKA EGLKKQVQTL 850
    VQKMKSDPRI NFQRDWKVIT LLIGTTDLCD YCTDSNLYSA TNFSAYLRDA 900
    LDFLHREVPR ALVNLVDFMN PIIFRQVFLG NQDKCPMTQA CSLCNCLLTV 950
    REDSPELARM KEVVKVYQTK IRELVESGRY DTREDFTIVL QPFLEEVKLA 1000
    FQDGIPDVSF FAPDCIHPNQ KFHSQLSRAL WNNMLQPLGK KMDFLDLTAD 1050
    TTLSCPSLEE PFLRTYRNSN YTYPANPAIE NWGSDFLCPE QGSSNSAPSS 1100
    VHQLRPADIK VVAALGDSLT TAVGAGAKNN SNWSTAWRGL SWSIGGDGTL 1150
    ETHTTLPNIL KKFNPHLHGF STGTQEEAAG LNVAVEGAIA QDMPSQARIL 1200
    VDRMKNSSEI NLDQDWKLIT IFVGSNDLCQ YCENPEAHSV KNYVQSLQQA 1250
    LDIFYKELPR AFINVVEIME LVGLRQIQGE KCAVTTQSIC PCFGRSLDNS 1300
    PELQEMKNIN RHFQSGSSLI TYHHQYMERE DFAVVVQPFF QSTVVPLDDK 1350
    GKPDLSFFSV DCFHFSERGH AEMAIALWNN MLEPVGQKQT YNNFTHSRTK 1400
    VKCPTSENPY FFTMRNSGLL RKEASHKTPS VPFWAVLVAA AASFIVGVVL 1450
    VMLWRTKKCS RREERPISLV ATVF 1474
    Length:1,474
    Mass (Da):165,157
    Last modified:October 31, 2006 - v1
    Checksum:iB22844CD69B35A60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ875604 mRNA. Translation: ABI74623.1.
    RefSeqiNP_001070653.1. NM_001077185.1.
    UniGeneiMdm.186.

    Genome annotation databases

    GeneIDi768082.
    KEGGimdo:768082.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ875604 mRNA. Translation: ABI74623.1 .
    RefSeqi NP_001070653.1. NM_001077185.1.
    UniGenei Mdm.186.

    3D structure databases

    ProteinModelPortali Q06HQ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 13616.ENSMODP00000019434.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 768082.
    KEGGi mdo:768082.

    Organism-specific databases

    CTDi 5350.

    Phylogenomic databases

    eggNOGi NOG311176.
    HOGENOMi HOG000115574.
    HOVERGENi HBG108263.
    InParanoidi Q06HQ7.
    KOi K14621.

    Family and domain databases

    Gene3Di 3.40.50.1110. 6 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 3 hits.
    [Graphical view ]
    PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dietary cholesterol increases intestinal phospholipase B gene expression in laboratory opossums."
      Chan J., Kushwaha R.S., Gluhak-Heinrich J., Donalson L.M., VandeBerg J.F., VandeBerg J.L.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPLB1_MONDO
    AccessioniPrimary (citable) accession number: Q06HQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3