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Q06HQ7

- PLB1_MONDO

UniProt

Q06HQ7 - PLB1_MONDO

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Protein

Phospholipase B1, membrane-associated

Gene
PLB1, PLB
Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031Nucleophile By similarity
Binding sitei477 – 4771Substrate; via amide nitrogen By similarity
Binding sitei516 – 5161Substrate By similarity
Active sitei1361 – 13611 By similarity
Active sitei1364 – 13641 By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
Synonyms:PLB
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
ProteomesiUP000002280: Unplaced

Subcellular locationi

Apical cell membrane; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 14321410Extracellular Reviewed predictionAdd
BLAST
Transmembranei1433 – 145321Helical; Reviewed predictionAdd
BLAST
Topological domaini1454 – 147421Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Chaini23 – 14741452Phospholipase B1, membrane-associatedPRO_0000324384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi185 – 1851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi711 – 7111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi813 – 8131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1070 – 10701N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi13616.ENSMODP00000019434.

Structurei

3D structure databases

ProteinModelPortaliQ06HQ7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati36 – 3503151Add
BLAST
Repeati365 – 7103462Add
BLAST
Repeati711 – 10693593Add
BLAST
Repeati1079 – 14173394Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 141713824 X 308-326 AA approximate repeatsAdd
BLAST
Regioni1418 – 146144Necessary for membrane localization By similarityAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ06HQ7.
KOiK14621.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06HQ7-1 [UniParc]FASTAAdd to Basket

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MRLQPSLLLL ALLLLKVTTI QATLGNNSWE EQTWPEGLKN FPFPCRPEEI     50
PFSPPSESVH SLRISDIRVV GAIGNLETTP DAKMVLKRQK WIERNQMQTC 100
MQVMTVLSDF IKKFSPSVLP LLCPSREKVL PRMRNEDLGP QARELVQHMK 150
KNQKLNFRND WKLINVFFNA QSQCHPCSSS QKEGNMTSSM QELIRLLDYL 200
QKEVPKAFVN LVDISELGNS IQSNNQENGP RDINEVCECP QEPSRLTTAV 250
QRWYYQNSWE KLLTSGRYDT KDTFTVIYQS FLHEMEPSVF PIKQTSGSSL 300
KKLSTALAVS LWNGMIDPTG KKEEFFTTGQ RIPVKCPTQK HPYLFTYRNN 350
NKLSSTPEIK RISETREGTE IRCPDMDPSN AVPTSVHHLR PADIKVIGAL 400
GDSLTAGNGA GSTPGNILDV LTQYRGLSWS IGGNENISTV TTLANILREF 450
NPSLIGFSTG TGNQNSAAAF LNQAVAGATS ETLPSQARRL VDLMKENKRI 500
NFQDDWKIVT LFIGGNDLCD SCSDPPRYSS QNFTAHIGKA LDILHAEVPR 550
VFVNLVTVLS IVSLRELYQE ESINCPRVIL RSLCPCVLKF KDNSTELTTL 600
IELNKQYQEE THRLVDSGRY DTRDDFTVVL QPFLEQVPMP KTPEGLPDSS 650
FFAPDCFHFH SKAHARVARS LWNNMLEPVG EKKKEENLEN IVDLICPSES 700
QPYLRTYKNS NYTYPTATPT SSPKPDYGSQ LWCEDRAPSA SFPTSVHALK 750
PADIQVVAAV GDSLTAGNGI GSKPDDLPDV ITQYRGLSYS AGGDASLESV 800
TTLPNILREF NMNLTGFAVG VGSADDANAF LNQAVPGAKA EGLKKQVQTL 850
VQKMKSDPRI NFQRDWKVIT LLIGTTDLCD YCTDSNLYSA TNFSAYLRDA 900
LDFLHREVPR ALVNLVDFMN PIIFRQVFLG NQDKCPMTQA CSLCNCLLTV 950
REDSPELARM KEVVKVYQTK IRELVESGRY DTREDFTIVL QPFLEEVKLA 1000
FQDGIPDVSF FAPDCIHPNQ KFHSQLSRAL WNNMLQPLGK KMDFLDLTAD 1050
TTLSCPSLEE PFLRTYRNSN YTYPANPAIE NWGSDFLCPE QGSSNSAPSS 1100
VHQLRPADIK VVAALGDSLT TAVGAGAKNN SNWSTAWRGL SWSIGGDGTL 1150
ETHTTLPNIL KKFNPHLHGF STGTQEEAAG LNVAVEGAIA QDMPSQARIL 1200
VDRMKNSSEI NLDQDWKLIT IFVGSNDLCQ YCENPEAHSV KNYVQSLQQA 1250
LDIFYKELPR AFINVVEIME LVGLRQIQGE KCAVTTQSIC PCFGRSLDNS 1300
PELQEMKNIN RHFQSGSSLI TYHHQYMERE DFAVVVQPFF QSTVVPLDDK 1350
GKPDLSFFSV DCFHFSERGH AEMAIALWNN MLEPVGQKQT YNNFTHSRTK 1400
VKCPTSENPY FFTMRNSGLL RKEASHKTPS VPFWAVLVAA AASFIVGVVL 1450
VMLWRTKKCS RREERPISLV ATVF 1474
Length:1,474
Mass (Da):165,157
Last modified:October 31, 2006 - v1
Checksum:iB22844CD69B35A60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ875604 mRNA. Translation: ABI74623.1.
RefSeqiNP_001070653.1. NM_001077185.1.
UniGeneiMdm.186.

Genome annotation databases

GeneIDi768082.
KEGGimdo:768082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ875604 mRNA. Translation: ABI74623.1 .
RefSeqi NP_001070653.1. NM_001077185.1.
UniGenei Mdm.186.

3D structure databases

ProteinModelPortali Q06HQ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 13616.ENSMODP00000019434.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 768082.
KEGGi mdo:768082.

Organism-specific databases

CTDi 5350.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi Q06HQ7.
KOi K14621.

Family and domain databases

Gene3Di 3.40.50.1110. 6 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dietary cholesterol increases intestinal phospholipase B gene expression in laboratory opossums."
    Chan J., Kushwaha R.S., Gluhak-Heinrich J., Donalson L.M., VandeBerg J.F., VandeBerg J.L.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPLB1_MONDO
AccessioniPrimary (citable) accession number: Q06HQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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