Q06FN6 (RPAL3_PELHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative DNA-directed RNA polymerase subunit alpha-like 3
Short name=Putative PEP 3
EC=2.7.7.6

Alternative name(s):
Putative plastid-encoded RNA polymerase subunit alpha 3
Short name=Putative RNA polymerase subunit alpha 3

Gene names

Name:	rpoAL3-A
Synonyms:	ORF365
AND
Name:	rpoAL3-B
Synonyms:	ORF365

Encoded on

Plastid; Chloroplast

Organism

Pelargonium hortorum (Common geranium)

Taxonomic identifier

4031 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Geraniales › Geraniaceae › Pelargonium

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00059
Subunit structure	In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Subcellular location	Plastid › chloroplast HAMAP MF_00059.
Sequence similarities	Belongs to the RNA polymerase alpha chain family.
Caution	Could be the product of a pseudogene. There are 3 rpoA-like genes in this organism (found in the inverted repeat). None of them are convincing as rpoA, and it may be that the functional gene is in the nucleus.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Chloroplast DNA-directed RNA polymerase Plastid
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Putative DNA-directed RNA polymerase subunit alpha-like 3 HAMAP MF_00059		PRO_0000296903

Sequences

	Sequence		Length	Mass (Da)
	Q06FN6 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 754375091C810A5F	FASTA	365	41,391
	10 20 30 40 50 60 MDFSEFSLEI TNNKNFADWD YVECKYFHKD FAYGRFALSP LTAKESRLLK KGLFEALLTE 70 80 90 100 110 120 ILCLRFTHAK IQNECVNLMN IVGIQESLDE ILKNFGKIIL TGKLEEFVGK GPFVAILDVR 130 140 150 160 170 180 GPLNAMAVDI ELPPGIKVEI ETQHIATITE PIPFVVELRI ELVSSTSKGE TGITDEEGFS 190 200 210 220 230 240 IDPNPPIRKV NSSIQGYEYG GQTFERLSIE MLTSLPIIPN EALLLGSMKI MNLFQIVLQA 250 260 270 280 290 300 KYLDYKELEK GIHVGVFCVS ALRAEQSKWI KTILEDALYM VGGRKHQGPL TDEEDDSIDS 310 320 330 340 350 360 NFTPVQNLDC RIESYEEEGQ TFQRLFLEIW TKSPTEPQEA LWEASAKILE LFSLFLQTSK ENEKD

References

[1]

"The complete chloroplast genome sequence of Pelargonium x hortorum: organization and evolution of the largest and most highly rearranged chloroplast genome of land plants." Chumley T.W., Palmer J.D., Mower J.P., Fourcade H.M., Calie P.J., Boore J.L., Jansen R.K. Mol. Biol. Evol. 23:2175-2190(2006) [PubMed: 16916942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Ringo White.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ897681 Genomic DNA. Translation: ABI17303.1. DQ897681 Genomic DNA. Translation: ABI17337.1.
RefSeq	YP_784111.1. NC_008454.1. YP_784145.1. NC_008454.1.
3D structure databases
ProteinModelPortal	Q06FN6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4362876. 4362970.
Phylogenomic databases
ProtClustDB	CHL00013.
Family and domain databases
HAMAP	MF_00059. RNApol_bact_RpoA. Divergent sequence. [Tree]
InterPro	IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
SUPFAM	SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

RPAL3_PELHO

Accession

Primary (citable) accession number: Q06FN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 31, 2006
Last modified:	May 31, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program