ID   RPAL2_PELHO             Reviewed;         322 AA.
AC   Q06FN4;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 14.
DE   RecName: Full=Putative DNA-directed RNA polymerase subunit alpha-like 2;
DE            Short=Putative PEP 2;
DE            EC=2.7.7.6;
DE   AltName: Full=Putative plastid-encoded RNA polymerase subunit alpha 2;
DE            Short=Putative RNA polymerase subunit alpha 2;
GN   Name=rpoAL2-A; Synonyms=ORF332;
GN   and
GN   Name=rpoAL2-B; Synonyms=ORF332;
OS   Pelargonium hortorum (Common geranium).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; Geraniales; Geraniaceae; Pelargonium.
OX   NCBI_TaxID=4031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Ringo White;
RX   PubMed=16916942; DOI=10.1093/molbev/msl089;
RA   Chumley T.W., Palmer J.D., Mower J.P., Fourcade H.M., Calie P.J.,
RA   Boore J.L., Jansen R.K.;
RT   "The complete chloroplast genome sequence of Pelargonium x hortorum:
RT   organization and evolution of the largest and most highly rearranged
RT   chloroplast genome of land plants.";
RL   Mol. Biol. Evol. 23:2175-2190(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core
CC       is composed of four subunits: alpha, beta, beta', and beta''. When
CC       a (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and
CC       basal transcription, whereas the C-terminal domain is involved in
CC       interaction with transcriptional regulators and with upstream
CC       promoter elements (By similarity).
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC   -!- CAUTION: Could be the product of a pseudogene. There are 3 rpoA-
CC       like genes in this organism (found in the inverted repeat). None
CC       of them are convincing as rpoA, and it may be that the functional
CC       gene is in the nucleus.
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DR   EMBL; DQ897681; ABI17301.1; -; Genomic_DNA.
DR   EMBL; DQ897681; ABI17339.1; -; Genomic_DNA.
DR   RefSeq; YP_784109.1; -.
DR   RefSeq; YP_784147.1; -.
DR   GeneID; 4362844; -.
DR   GeneID; 4362953; -.
DR   BRENDA; 2.7.7.6; 280179.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR   GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:HAMAP.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-dependent; IEA:HAMAP.
DR   HAMAP; MF_00059; atypical; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   Gene3D; G3DSA:2.170.120.12; RNAP_insert; 1.
PE   5: Uncertain;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Plastid; Transcription; Transferase.
FT   CHAIN         1    322       Putative DNA-directed RNA polymerase
FT                                subunit alpha-like 2.
FT                                /FTId=PRO_0000296902.
FT   REGION        1    232       Alpha N-terminal domain (alpha-NTD) (By
FT                                similarity).
FT   REGION      280    322       Alpha C-terminal domain (alpha-CTD) (By
FT                                similarity).
SQ   SEQUENCE   322 AA;  36122 MW;  C0DB7394BBA2A4D9 CRC64;
     MSNPNNGAEW QQVEADQLDS GLYYGRFALS PLTAKQASLL KKGLPEALLT EILCLRFTHA
     KIQNECVNLM NIVGIQESLD EILKNFGKII LTGKLEEFVG KGPFVAILDV RGPLNAMAVD
     IELPPGIKVE IETQHIATIT EPIPFVVELK IELVSSTSKG ETGITDEEGF SIDPNPPIQK
     VDTSIQCYDY QGEPFQTLFL DIWTDRTIHP HEALAQASRK IFGLLSLVFQ AEYFQYNELE
     NGLRYGKFCL YPMTKEQFQW IQTALNEALA LDMSGESKHE GPVTDEEGDS IDPTFTPVQK
     WDITMNSYQY SGETFQGLLS RF
//