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Protein

Phenylethanolamine N-methyltransferase

Gene

PNMT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts noradrenaline to adrenaline.By similarity

Catalytic activityi

S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine.

Pathwayi: (R)-adrenaline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline.
Proteins known to be involved in this subpathway in this organism are:
  1. Phenylethanolamine N-methyltransferase (PNMT)
This subpathway is part of the pathway (R)-adrenaline biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline, the pathway (R)-adrenaline biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351S-adenosyl-L-methionineBy similarity
Binding sitei40 – 401S-adenosyl-L-methionineBy similarity
Binding sitei85 – 851S-adenosyl-L-methionineBy similarity
Binding sitei101 – 1011S-adenosyl-L-methionineBy similarity
Binding sitei106 – 1061S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei219 – 2191SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SSC-209905. Catecholamine biosynthesis.
UniPathwayiUPA00749; UER00736.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylethanolamine N-methyltransferase (EC:2.1.1.28)
Short name:
PNMTase
Alternative name(s):
Noradrenaline N-methyltransferase
Gene namesi
Name:PNMT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 12

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Phenylethanolamine N-methyltransferasePRO_0000289662Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ06AU9.

Expressioni

Gene expression databases

BgeeiENSSSCG00000017501.
GenevisibleiQ06AU9. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000018549.

Structurei

3D structure databases

ProteinModelPortaliQ06AU9.
SMRiQ06AU9. Positions 13-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 802S-adenosyl-L-methionine bindingBy similarity
Regioni158 – 1592S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IJGY. Eukaryota.
ENOG4111F9R. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiQ06AU9.
KOiK00553.
OMAiFAWAQKK.
OrthoDBiEOG091G0EF7.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06AU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTGQSHAA DAAPDSDPGQ AAVALAYQHF EPRAYLRNNY APPRGDLSSP
60 70 80 90 100
DGVGPWKLRC LAQTFATGEV SGRALIDIGS GPTIYQLLSA CAHFEDITMT
110 120 130 140 150
DFLEVNRQEL GLWLREEPGA FDWSVYSQHV CLIEGKGESC QEKERQLRAR
160 170 180 190 200
VKRILPIDVH QPQPLGTGSL APLPADALVS AFCLEAVSPD LASFQRALDH
210 220 230 240 250
ITTLLRSGGH LLLIGALEES WYLAGEARLA VVPVCEEEVR EALARSGYEV
260 270 280
RDLRTYVMPA HLRTGVDDVK GIFFAWAQKK VGV
Length:283
Mass (Da):30,879
Last modified:October 31, 2006 - v1
Checksum:i15ADE065A8F4B8C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ917626 mRNA. Translation: ABI97171.1.
RefSeqiNP_001116636.1. NM_001123164.1.
UniGeneiSsc.13910.

Genome annotation databases

EnsembliENSSSCT00000019054; ENSSSCP00000018549; ENSSSCG00000017501.
GeneIDi100144479.
KEGGissc:100144479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ917626 mRNA. Translation: ABI97171.1.
RefSeqiNP_001116636.1. NM_001123164.1.
UniGeneiSsc.13910.

3D structure databases

ProteinModelPortaliQ06AU9.
SMRiQ06AU9. Positions 13-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000018549.

Proteomic databases

PaxDbiQ06AU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000019054; ENSSSCP00000018549; ENSSSCG00000017501.
GeneIDi100144479.
KEGGissc:100144479.

Organism-specific databases

CTDi5409.

Phylogenomic databases

eggNOGiENOG410IJGY. Eukaryota.
ENOG4111F9R. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiQ06AU9.
KOiK00553.
OMAiFAWAQKK.
OrthoDBiEOG091G0EF7.
TreeFamiTF313114.

Enzyme and pathway databases

UniPathwayiUPA00749; UER00736.
ReactomeiR-SSC-209905. Catecholamine biosynthesis.

Gene expression databases

BgeeiENSSSCG00000017501.
GenevisibleiQ06AU9. SS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNMT_PIG
AccessioniPrimary (citable) accession number: Q06AU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 31, 2006
Last modified: September 7, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.